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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Isolation and Characterization of a new Dgalactose- Binding Lectin from Sambucus Racemosa L.

Author(s): M. Angeles Rojo, Lucia Citores, Pilar Jimenez, J. Miguel Ferreras, F. Javier Arias, Enrique Mendez and Tomas Girbes

Volume 10, Issue 3, 2003

Page: [287 - 293] Pages: 7

DOI: 10.2174/0929866033478979

Price: $65

Abstract

A new acidic lectin from red elder (Sambucus racemosa L.) bark has been isolated by affinity chromatography and gel filtration. Noteworthy, and in contrast to other Sambucus species, red elder bark lacks acidic non-toxic type 2 ribosome-inactivating proteins but has basic ribosome-inactivating protein activities. The new lectin (SRLbm) shows specificity for N-Ac-Galactosamine / D-Galactose and has an apparent Mr of 30,000. The N-terminal amino acid sequence displays a close homology with other lectins and B chains of non-toxic type 2 ribosome-inactivating proteins nigrins and ebulins present in other Sambucus species. SRLbm triggers red blood cell agglutination in the range 4-12 μg/ml.

Keywords: lectin, d-galactose, sambucus racemosa, ribosome-inactivating protein, bark


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