Abstract
The interactions between an oligomeric heat-shock protein, α-crystallin, and its individual subunits with unfolded proteins were monitored by surface plasmon resonance. Immobilization at the sensor chip allowed us for the first time to study isolated α-crystallin subunits under physiological conditions. We observe that these subunits, in contrast to α-crystallin oligomers, do not bind unfolded protein. Our data indicate that quaternary structure of α-crystallin is necessary for its chaperone-like activity.
Keywords: crystallin, small heat shock protein, chaperone-like activity, surface plasmon resonance
Protein & Peptide Letters
Title: Quaternary Structure Of α-Crystallin Is Necessary For The Binding Of Unfolded Proteins: A Surface Plasmon Resonance Study
Volume: 11 Issue: 1
Author(s): Sergiy V. Avilov, Nataliya A. Aleksandrova and Alexander P. Demchenko
Affiliation:
Keywords: crystallin, small heat shock protein, chaperone-like activity, surface plasmon resonance
Abstract: The interactions between an oligomeric heat-shock protein, α-crystallin, and its individual subunits with unfolded proteins were monitored by surface plasmon resonance. Immobilization at the sensor chip allowed us for the first time to study isolated α-crystallin subunits under physiological conditions. We observe that these subunits, in contrast to α-crystallin oligomers, do not bind unfolded protein. Our data indicate that quaternary structure of α-crystallin is necessary for its chaperone-like activity.
Export Options
About this article
Cite this article as:
Avilov V. Sergiy, Aleksandrova A. Nataliya and Demchenko P. Alexander, Quaternary Structure Of α-Crystallin Is Necessary For The Binding Of Unfolded Proteins: A Surface Plasmon Resonance Study, Protein & Peptide Letters 2004; 11 (1) . https://dx.doi.org/10.2174/0929866043478437
DOI https://dx.doi.org/10.2174/0929866043478437 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Medical Complications of Anorexia Nervosa and Their Treatments
Current Women`s Health Reviews Fatty Acid-Mediated Inhibition of Metal Binding to the Multi-Metal Site on Serum Albumin: Implications for Cardiovascular Disease
Current Topics in Medicinal Chemistry Osteoporosis Entwined with Cardiovascular Disease: The Implication of Osteoprotegerin and the Example of Statins
Current Medicinal Chemistry Advanced Trans-Epithelial Drug Delivery Devices
Current Pharmaceutical Biotechnology Functions of Ghrelin in Brain, Gut and Liver
CNS & Neurological Disorders - Drug Targets Curcumin Nanomedicine: A Road to Cancer Therapeutics
Current Pharmaceutical Design Chemokines as Potential Therapeutic Targets in Atherosclerosis
Current Drug Targets The Targets of Curcumin
Current Drug Targets Regulators of Stem Cells Proliferation in Tissue Regeneration
Current Stem Cell Research & Therapy Retrospective, Observation Study: Quantitative and Qualitative Effect of Ezetimibe and HMG-CoA Reductase Inhibitors on LDL-Cholesterol: Are There Disappearance Thresholds for Small, Dense LDL and IDL?
Recent Patents on Cardiovascular Drug Discovery Tumor Necrosis Factor Alpha in Ulcerative Colitis and Diverticular Disease Associated Colitis
Endocrine, Metabolic & Immune Disorders - Drug Targets Disorder in Milk Proteins: α -Lactalbumin. Part A. Structural Properties and Conformational Behavior
Current Protein & Peptide Science Mitochondrial Proteases as Emerging Pharmacological Targets
Current Pharmaceutical Design GSK3 Inhibitors in the Therapeutic Development of Diabetes, Cancer and Neurodegeneration: Past, Present and Future
Current Pharmaceutical Design <i>Astragalus hamosus</i> Acts as an Insulin Sensitizer in the Treatment of Polycystic Ovary Syndrome Rat Models by Affecting <i>IRS1</i> Expression
Endocrine, Metabolic & Immune Disorders - Drug Targets Gender Differences in Autonomic Control of the Cardiovascular System
Current Pharmaceutical Design Differential Effects of Efavirenz and Lopinavir/Ritonavir on Human Adipocyte Differentiation, Gene Expression and Release of Adipokines and Pro-Inflammatory Cytokines.
Current HIV Research Protective Effect of Alpha-Tocopherol in Deltamethrin Induced Immunotoxicity
Endocrine, Metabolic & Immune Disorders - Drug Targets How to Assess Sympathetic Nervous System Activity in Clinical Practice
Current Clinical Pharmacology Effectiveness and Efficiency of Platelet Rich Plasma in the Treatment of Diabetic Ulcers
Current Pharmaceutical Biotechnology