Abstract
Intein-mediated protein splicing is facilitated by four separate but coordinated nucleophilic displacement reactions that result in the excision of the intein and the ligation of the flanking polypeptides, called the exteins. These reactions are catalyzed by the intein plus the first downstream extein amino acid without the assistance of cofactors or auxiliary enzymes. Non-canonical inteins missing conserved nucleophilic residues at the N- or C-terminus likely splice using variations of the standard mechanism.
Keywords: intein, protein splicing, expressed protein ligation, protein purification, thioester, succinimide
Protein & Peptide Letters
Title: The Mechanism of Intein-Mediated Protein Splicing: Variations on a Theme
Volume: 12 Issue: 8
Author(s): Kenneth V. Mills and Francine B. Perler
Affiliation:
Keywords: intein, protein splicing, expressed protein ligation, protein purification, thioester, succinimide
Abstract: Intein-mediated protein splicing is facilitated by four separate but coordinated nucleophilic displacement reactions that result in the excision of the intein and the ligation of the flanking polypeptides, called the exteins. These reactions are catalyzed by the intein plus the first downstream extein amino acid without the assistance of cofactors or auxiliary enzymes. Non-canonical inteins missing conserved nucleophilic residues at the N- or C-terminus likely splice using variations of the standard mechanism.
Export Options
About this article
Cite this article as:
Mills V. Kenneth and Perler B. Francine, The Mechanism of Intein-Mediated Protein Splicing: Variations on a Theme, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864337
DOI https://dx.doi.org/10.2174/0929866054864337 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers