Chemical Synthesis of Proteins and Circular Peptides Using Nα-(1-Phenyl- 2-Mercaptoethyl) Auxiliaries

Title: Chemical Synthesis of Proteins and Circular Peptides Using Nα-(1-Phenyl- 2-Mercaptoethyl) Auxiliaries

Volume: 12 Issue: 8

Author(s): Paolo Botti and Sylvie Tchertchian

Affiliation:

Keywords: native chemical ligation, peptide cyclization, auxiliary group, protecting group

Abstract: An overview of the applications of Nα-(1-phenyl-2-mercaptoethyl) auxiliary is presented. We describe the on resin preparation (C α-carboxy and thioester) of Nα-auxiliary derivatives of glycine and the synthesis and incorporation of preformed Nα-auxiliary derivatives of glycine and alanine with the protection schemes, including the thiazolidine strategy for SPPS. Such approaches allowed the synthesis of the protein cytochrome b562 as well as native circular peptides after successful removal of the auxiliary.

Cite this article as:

Botti Paolo and Tchertchian Sylvie, Chemical Synthesis of Proteins and Circular Peptides Using Nα-(1-Phenyl- 2-Mercaptoethyl) Auxiliaries, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864300