Abstract
The Surface Plasmon Resonance Imaging (SPRI) sensor for papain determination based on the interaction between the immobilized cystatin and aqueous papain solution has been developed. The development of the sensor is a stage in the development of the sensor for the determination of cystein proteinases of the papain group. Cystatin was immobilized onto a gold chip by means of a thiol underlayer (cysteamine) and EDS/NHS reaction. Conditions of cystatin- papain interaction were optimized (pH, time of interaction and cystatin concentration). The developed sensor works within the range of 1 – 10 ng ml-1. However, the precision of measurements (RSD equal to 45%) should be improved. The response of the sensor to papain is specific. This was checked using BSA as a reference.
Keywords: surface plasmon resonance imaging, papain, cystatin
Protein & Peptide Letters
Title: The Surface Plasmon Resonance Imaging Sensor for Papain Based on Immobilized Cystatin
Volume: 14 Issue: 5
Author(s): E. Gorodkiewicz
Affiliation:
Keywords: surface plasmon resonance imaging, papain, cystatin
Abstract: The Surface Plasmon Resonance Imaging (SPRI) sensor for papain determination based on the interaction between the immobilized cystatin and aqueous papain solution has been developed. The development of the sensor is a stage in the development of the sensor for the determination of cystein proteinases of the papain group. Cystatin was immobilized onto a gold chip by means of a thiol underlayer (cysteamine) and EDS/NHS reaction. Conditions of cystatin- papain interaction were optimized (pH, time of interaction and cystatin concentration). The developed sensor works within the range of 1 – 10 ng ml-1. However, the precision of measurements (RSD equal to 45%) should be improved. The response of the sensor to papain is specific. This was checked using BSA as a reference.
Export Options
About this article
Cite this article as:
Gorodkiewicz E., The Surface Plasmon Resonance Imaging Sensor for Papain Based on Immobilized Cystatin, Protein & Peptide Letters 2007; 14 (5) . https://dx.doi.org/10.2174/092986607780782803
DOI https://dx.doi.org/10.2174/092986607780782803 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Prevalence of Frailty in Patients Admitted to Hospital with Vertebral Fragility Fractures
Current Rheumatology Reviews Anti-inflammatory Effects of Rosuvastatin in Healthy Subjects: A Prospective Longitudinal Study
Current Pharmaceutical Design Monoclonal Antibody-based Genetic Immunotherapy
Current Gene Therapy Gene Expression Profiles in Human Autoimmune Disease
Current Pharmaceutical Design Use of Recombinant Human Erythropoietin as an Antianemic and Performance Enhancing Drug
Current Pharmaceutical Biotechnology IL-17 Axis Driven Inflammation in Non-Alcoholic Fatty Liver Disease Progression
Current Drug Targets Chymase Inhibitors
Current Pharmaceutical Design Herbals and Natural Dietary Supplements in Psychiatric Practice
Recent Patents on CNS Drug Discovery (Discontinued) Enzymatic Processing of <i>Omega</i> 3 Long Chain Polyunsaturated Fatty Acid Oils
Current Nutrition & Food Science Glycomics: Relevance for Personalized Medicine
Current Pharmacogenomics and Personalized Medicine Peripheral Benzodiazepine Receptor (PBR) New Insight in Cell Proliferation and Cell Differentiation Review
Current Clinical Pharmacology Multi-target Activities of Selected Alkaloids and Terpenoids
Mini-Reviews in Organic Chemistry Mediterranean Diet and Longevity
Current Nutrition & Food Science Benzophenone Esters and Sulfonates: Synthesis and their Potential as Antiinflammatory Agents
Medicinal Chemistry How to Manage the Infectious Risk under Anti-TNF in Inflammatory Bowel Disease
Current Drug Targets Evaluation of Dendrimer Safety and Efficacy through Cell Line Studies
Current Drug Targets CARD Proteins as Therapeutic Targets in Cancer
Current Drug Targets Giant Cell Arteritis - Beyond the Headache
Current Rheumatology Reviews Clinical Pharmacokinetics of Ibuprofen Arginine
Current Clinical Pharmacology Fruit as Potent Natural Antioxidants and Their Biological Effects
Current Pharmaceutical Biotechnology