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Mini-Reviews in Medicinal Chemistry


ISSN (Print): 1389-5575
ISSN (Online): 1875-5607

Receptors and Enzymes for Medical Sensing of L-Glutamate

Author(s): Ayumi Hirano and Masao Sugawara

Volume 6, Issue 10, 2006

Page: [1091 - 1100] Pages: 10

DOI: 10.2174/138955706778560102

Price: $65


Medical sensing systems using isolated or intact glutamate receptor (GluR) ion channels and glutamate oxidase (GluOx) are discussed for L-glutamate, one of the principal neurotransmitter in the central nervous systems of mammalian brain, and related agonists. The GluR-based sensing system used for the evaluation of signal transduction ability of GluR channels demonstrate that the agonist selectivity based on the signal transduction ability is not parallel to that of the binding assay. On the other hand, the appropriate design of the enzyme system, namely glutamate oxidase (GluOx), in combination with horseradish peroxidase (HRP), enables to real-time monitoring of L-glutamate in vivo and in vitro and also to visualize its release in submerged, acute mouse hippocampal slices.

Keywords: Glutamate receptor ion channels, Glutamate oxidase, acute brain slice, gonist selectivity, patch sensor, visualization of glutamate release, micro sensor, agonist selectivity

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