Polycaprolactone Fibres as a Potential Delivery System for Collagen to Support Bone Regeneration

Sarah      E. McNeil; Helen      R. Griffiths; Yvonne      Perrie

Abstract

Poly(-caprolactone) (PCL) is biocompatible, non-immunogenic non-toxic and slowly degrades, allowing sufficient time for tissue regeneration. PCL has the potential for application in bone and cartilage repair as it may provide the essential structure required for bone regeneration, however, an ideal scaffold system is still undeveloped. PCL fibres were prepared using the gravity spinning technique, in which collagen was either incorporated into or coated onto the ‘as-spun’ fibres, in order to develop novel biodegradable polymer fibres which will effectively deliver collagen and support the attachment and proliferation of human osteoblast (HOB) cells for bone regeneration. The physical and mechanical characteristics and cell fibre interactions were analysed. The PCL fibres were found to be highly flexible and inclusion of collagen did not alter the mechanical properties of PCL fibres. Overall, HOB cells were shown to effectively adhere and proliferate on all fibre platforms tested, although proliferation rates were enhanced by surface coating PCL fibres with collagen compared to PCL fibres incorporating collagen and PCL only fibres. These findings highlight the potential of using gravity spun PCL fibres as a delivery platform for extracellular matrix proteins, such as collagen, in order to enhance cell adherence and proliferation for tissue repair.

Keywords: Drug delivery platforms, tissue engineering, polycaprolactone, collagen, human oesteoblast cells, bone repair, Bone Regeneration, congenital defects, biocompatible, Chemical cross-link