Eph/Ephrin Membrane Proteins: A Mammalian Expression Vector pTIg- BOS-Fc Allowing Rapid Protein Purification

Title: Eph/Ephrin Membrane Proteins: A Mammalian Expression Vector pTIg- BOS-Fc Allowing Rapid Protein Purification

Volume: 13 Issue: 2

Author(s): Bryan W. Day, Fiona M. Smith, Ke Chen, Jennifer K. McCarron, Nirmitha I. Herath, Martin Lackmann and Andrew W. Boyd

Affiliation:

Keywords: Crystallography, protein expression, purification, pTIg-BOS-Fc

Abstract: There is an urgent need for high purity, single chain, fully functional Eph/ephrin membrane proteins. This report outlines the pTIg-BOS-Fc vector and purification approach resulting in rapid increased production of fully functional single chain extracellular proteins that were isolated with high purity and used in structure-function analysis and pre-clinical studies.

Cite this article as:

Day W. Bryan, Smith M. Fiona, Chen Ke, McCarron K. Jennifer, Herath I. Nirmitha, Lackmann Martin and Boyd W. Andrew, Eph/Ephrin Membrane Proteins: A Mammalian Expression Vector pTIg- BOS-Fc Allowing Rapid Protein Purification, Protein & Peptide Letters 2006; 13(2) . https://dx.doi.org/10.2174/092986606775101625