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Current Nanoscience

Editor-in-Chief

ISSN (Print): 1573-4137
ISSN (Online): 1875-6786

Nano-Mechanics and Membrane Receptor Mapping of CD8 + T Cells (Supplementry Material)

Author(s): Yangzhe Wu, Yi Hu, Hongsong Lu, Jiye Cai, Xianhui He and Hongxia Zhao

Volume 5, Issue 4, 2009

Page: [465 - 469] Pages: 5

DOI: 10.2174/157341309789378096

Price: $65

Abstract

The investigation of nano-mechanical properties and nano-architectures of CD8 + T cells activated in vitro will help further interpret the immune response/recognition of these cells at nanoscale resolution. In this work, the local biophysical properties of human peripheral blood CD8 + T cells were obtained, revealing the adhesion force of membrane was increased after CD8 + T cells were stimulated with phorbol dibutyrate plus ionomycin, though the measured local stiffness for resting and activated cells was similar. The 3-D distribution pattern and grayscale map together revealed that membrane receptors (CD8 and activation marker CD69) distributed non-uniformly on the membrane and were shown as micro-, nano-scale clusters or domains. This work describes a relatively simple approach for exploiting the distribution pattern of receptor molecules on a single T cell surface.

Keywords: Force spectroscopy, molecule recognition, receptor mapping, nano-mechanics, CD8 + T cell


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