Abstract
There is rapid development in the field of protein microarray technology with the promise of important advancements in the near future. Protein microarrays have been reportedly successful in serum tumor marker profiling as well as in drug discovery and medicinal chemistry when the effect of small molecules in protein-protein interaction is studied. Some of the bottlenecks of the technology are protein instability, problems with immobilization and stabilization of proteins to the corresponding surface, as well as aspecific and /or not preferred interactions and the lack of protein amplification techniques to generate sufficient amounts of low abundance proteins. For the time being, the number of genes in RNA expression chips is significantly greater than the number of proteins available for microchip based analysis of gene expression at the protein level. The automation and standardization routinely used with nucleic acid microarrays is not yet available in their protein chip counterparts. One of the emerging applications of protein microchips is biomarker discovery via chromatographic surface-based protein array techniques, which is applicable to minute amounts of samples with excellent detection limits using mass spectrometry based interrogation. In this paper the advantages, technical limitations and main biomedical application of protein microarrays are reviewed.
Current Medicinal Chemistry
Title: Biomedical Applications of Protein Microarrays
Volume: 16 Issue: 22
Author(s): Sandor Spisak and Andras Guttman
Affiliation:
Abstract: There is rapid development in the field of protein microarray technology with the promise of important advancements in the near future. Protein microarrays have been reportedly successful in serum tumor marker profiling as well as in drug discovery and medicinal chemistry when the effect of small molecules in protein-protein interaction is studied. Some of the bottlenecks of the technology are protein instability, problems with immobilization and stabilization of proteins to the corresponding surface, as well as aspecific and /or not preferred interactions and the lack of protein amplification techniques to generate sufficient amounts of low abundance proteins. For the time being, the number of genes in RNA expression chips is significantly greater than the number of proteins available for microchip based analysis of gene expression at the protein level. The automation and standardization routinely used with nucleic acid microarrays is not yet available in their protein chip counterparts. One of the emerging applications of protein microchips is biomarker discovery via chromatographic surface-based protein array techniques, which is applicable to minute amounts of samples with excellent detection limits using mass spectrometry based interrogation. In this paper the advantages, technical limitations and main biomedical application of protein microarrays are reviewed.
Export Options
About this article
Cite this article as:
Spisak Sandor and Guttman Andras, Biomedical Applications of Protein Microarrays, Current Medicinal Chemistry 2009; 16 (22) . https://dx.doi.org/10.2174/092986709788803141
DOI https://dx.doi.org/10.2174/092986709788803141 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
Call for Papers in Thematic Issues
Advances in Medicinal Chemistry: From Cancer to Chronic Diseases.
The broad spectrum of the issue will provide a comprehensive overview of emerging trends, novel therapeutic interventions, and translational insights that impact modern medicine. The primary focus will be diseases of global concern, including cancer, chronic pain, metabolic disorders, and autoimmune conditions, providing a broad overview of the advancements in ...read more
Approaches to the treatment of chronic inflammation
Chronic inflammation is a hallmark of numerous diseases, significantly impacting global health. Although chronic inflammation is a hot topic, not much has been written about approaches to its treatment. This thematic issue aims to showcase the latest advancements in chronic inflammation treatment and foster discussion on future directions in this ...read more
Cellular and Molecular Mechanisms of Non-Infectious Inflammatory Diseases: Focus on Clinical Implications
The Special Issue covers the results of the studies on cellular and molecular mechanisms of non-infectious inflammatory diseases, in particular, autoimmune rheumatic diseases, atherosclerotic cardiovascular disease and other age-related disorders such as type II diabetes, cancer, neurodegenerative disorders, etc. Review and research articles as well as methodology papers that summarize ...read more
Chalcogen-modified nucleic acid analogues
Chalcogen-modified nucleosides, nucleotides and oligonucleotides have been of great interest to scientific research for many years. The replacement of oxygen in the nucleobase, sugar or phosphate backbone by chalcogen atoms (sulfur, selenium, tellurium) gives these biomolecules unique properties resulting from their altered physical and chemical properties. The continuing interest in ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Determining the Relative Gene Expression Level of Hypoxia Related Genes in Different Cancer Cell Lines
Current Molecular Pharmacology Sexual Dimorphism in Autoimmune Disease
Current Molecular Medicine Reproductive Toxicology of Environmental Toxicants: Emerging Issues and Concerns
Current Pharmaceutical Design Metalloprotein Inhibitors for the Treatment of Human Diseases
Current Topics in Medicinal Chemistry Natural Sourced Inhibitors of EGFR, PDGFR, FGFR and VEGFRMediated Signaling Pathways as Potential Anticancer Agents
Current Medicinal Chemistry Mechanisms of Hormonal Regulation of Sertoli Cell Development and Proliferation: A Key Process for Spermatogenesis
Current Molecular Pharmacology NFAT Gene Family in Inflammation and Cancer
Current Molecular Medicine Progress in Topical siRNA Delivery Approaches for Skin Disorders
Current Pharmaceutical Design Monofunctional Platinum (PtII) Compounds – Shifting the Paradigm in Designing New Pt-based Anticancer Agents
Current Medicinal Chemistry Protein Tyrosine Signaling and its Potential Therapeutic Implications in Carcinogenesis
Current Pharmaceutical Design Editorial (Hot Topic Potential Value and Limitation of Dual Inhibitors of PI3K and mTOR in the Treatment of Cancer)
Current Cancer Drug Targets Targeting the Tumor Stroma with Peroxisome Proliferator Activated Receptor (PPAR) Agonists
Anti-Cancer Agents in Medicinal Chemistry Role of NUCB2/nesfatin-1 as a Possible Biomarker
Current Pharmaceutical Design Enzymatic regulation and functional relevance of NOX5
Current Pharmaceutical Design Connection between JAK/STAT and PPARγ Signaling During the Progression of Multiple Sclerosis: Insights into the Modulation of T-Cells and Immune Responses in the Brain
Current Molecular Pharmacology Targeted Cancer Therapy; Nanotechnology Approaches for Overcoming Drug Resistance
Current Medicinal Chemistry Targeting CD4 to Disrupt Signaling Through Membrane Rafts: Towards a Raft-Based Therapeutics
Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Discontinued) IGF Signaling Pathway as a Selective Target of Familial Breast Cancer Therapy
Current Molecular Medicine Oncogenomics
Current Drug Metabolism Metabolomics: A Revolution for Novel Cancer Marker Identification
Combinatorial Chemistry & High Throughput Screening