Abstract
Establishment of a saliva protein/peptide signature will provide important information for clinical diagnostics and prognosis of human disease. We digested human whole saliva with trypsin to create a tryptic digest salivary peptidome. Proteins/peptides were subsequently identified by high throughput tandem mass spectrometry in conjunction with database searching. Sixty-three saliva peptides corresponding to twenty-two saliva proteins were identified. Thirty of sixty-three saliva peptides with non-specific tryptic cleavage sites were derived from proline-rich proteins, mucin 7, statherin and collagen. Several peptides derived from proline-rich proteins exhibit proline (Pro) - glutamine (Gln) C-termini (- PQ C-termini). Seven peptides with -PQ C-termini were identified in undigested whole saliva, suggesting that peptides with -PQ C-termini indigenously exist in human saliva. Peptides with -PQ C-termini are known to bind oral bacteria and exhibit properties characteristic of innate-immunity peptides. Thus, a saliva peptidome containing peptides with -PQ Ctermini, as presented here, may reinforce the development of innate-immunity-related disease monitoring using noninvasive saliva samples and mass spectrometry-based techniques.
Keywords: Mass spectrometry, peptidome, human whole saliva, tryptic digest
Combinatorial Chemistry & High Throughput Screening
Title: Profiling Human Saliva Endogenous Peptidome via a High Throughput MALDI-TOF-TOF Mass Spectrometry
Volume: 12 Issue: 5
Author(s): Chun-Ming Huang and Wenhong Zhu
Affiliation:
Keywords: Mass spectrometry, peptidome, human whole saliva, tryptic digest
Abstract: Establishment of a saliva protein/peptide signature will provide important information for clinical diagnostics and prognosis of human disease. We digested human whole saliva with trypsin to create a tryptic digest salivary peptidome. Proteins/peptides were subsequently identified by high throughput tandem mass spectrometry in conjunction with database searching. Sixty-three saliva peptides corresponding to twenty-two saliva proteins were identified. Thirty of sixty-three saliva peptides with non-specific tryptic cleavage sites were derived from proline-rich proteins, mucin 7, statherin and collagen. Several peptides derived from proline-rich proteins exhibit proline (Pro) - glutamine (Gln) C-termini (- PQ C-termini). Seven peptides with -PQ C-termini were identified in undigested whole saliva, suggesting that peptides with -PQ C-termini indigenously exist in human saliva. Peptides with -PQ C-termini are known to bind oral bacteria and exhibit properties characteristic of innate-immunity peptides. Thus, a saliva peptidome containing peptides with -PQ Ctermini, as presented here, may reinforce the development of innate-immunity-related disease monitoring using noninvasive saliva samples and mass spectrometry-based techniques.
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Cite this article as:
Huang Chun-Ming and Zhu Wenhong, Profiling Human Saliva Endogenous Peptidome via a High Throughput MALDI-TOF-TOF Mass Spectrometry, Combinatorial Chemistry & High Throughput Screening 2009; 12 (5) . https://dx.doi.org/10.2174/138620709788489019
DOI https://dx.doi.org/10.2174/138620709788489019 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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