Login Register Cart 0
Generic placeholder image

Current HIV Research

Editor-in-Chief

ISSN (Print): 1570-162X
ISSN (Online): 1873-4251

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Case Report

Identification of a New HIV-1 Circulating Recombinant Form CRF112_01B Strain in Baoding City, Hebei Province, China

Author(s): Hao Wang, Xuanhe Zhao, Miaomiao Su, Juan Meng, Weiguang Fan and Penghui Shi*

Volume 20, Issue 6, 2022

Published on: 15 November, 2022

Page: [485 - 491] Pages: 7

DOI: 10.2174/1570162X21666221027122528

Price: $65

Abstract

Background: A large number of HIV-1 recombinants that originated from CRF01_AE and B strains are constantly emerging in men who have sex with men populations in China and deserve more attention and further monitoring.

Objective: To analyze the near-full-length genome structure and recombination characteristics of a new HIV-1 strain (BD226AJ) detected in Baoding City and determine its subtype.

Case Representation: Viral RNA was extracted from a blood sample collected from an infected individual and reverse transcribed to cDNA. Two overlapping segments of the HIV-1 genome were amplified using a near-endpoint dilution method and sequenced. Recombinant breakpoints were determined using RIP, jpHMM, and SimPlot 3.5.1 software. MEGA v6.0 was used to construct a neighbor-joining phylogenetic tree to determine the homology relationships of this strain.

Results and Discussion: We obtained 8830 nucleotides (nt) of the HIV-1 genome sequence by amplification and sequencing, and four recombinant fragments were identified by recombination analysis, namely CRF01_AE (HXB2, 823–4224 nt), subtype B (HXB2, 4225–5991 nt), CRF01_AE (HXB2, 5992–9295 nt), and subtype B (HXB2, 9296–9406 nt). The BLAST results showed that 96% of the sequence was similar to CRF112_01B. The jpHMM results confirmed that BD226AJ was the CRF112_01B strain.

Conclusion: Our results confirm the first epidemic of CRF112_01B in Hebei Province. This finding suggests that HIV-1 CRF112_01B may have been introduced into Hebei by men who have sex with men and indicates that the epidemic trend of this strain should be closely monitored.

Keywords: HIV, recombination, near-full-length genome, CRF112_01B, nucleotides, gene polymorphisms.

« Previous Next »
Graphical Abstract

[1]
Yebra G, Frampton D, Gallo CT, et al. A high HIV-1 strain variability in London, UK, revealed by full-genome analysis: Results from the ICONIC project. PLoS One 2018; 13(2): e0192081.
[http://dx.doi.org/10.1371/journal.pone.0192081] [PMID: 29389981]
[2]
Ndung’u T, Weiss RA. On HIV diversity. AIDS 2012; 26(10): 1255-60.
[http://dx.doi.org/10.1097/QAD.0b013e32835461b5] [PMID: 22706010]
[3]
Anastassopoulou C, Kostrikis L. Global genetic variation of HIV-1 infection. Curr HIV Res 2006; 4(3): 365-73.
[http://dx.doi.org/10.2174/157016206777709456] [PMID: 16842087]
[4]
Hemelaar J. Implications of HIV diversity for the HIV-1 pandemic. J Infect 2013; 66(5): 391-400.
[http://dx.doi.org/10.1016/j.jinf.2012.10.026] [PMID: 23103289]
[5]
Li Y, Miao J, Miao Z, et al. Identification of a novel HIV type 1 Circulating Recombinant Form (CRF86_BC) among heterosexuals in Yunnan, China. AIDS Res Hum Retroviruses 2017; 33(3): 279-83.
[http://dx.doi.org/10.1089/aid.2016.0188] [PMID: 27593341]
[6]
Zhang Y, Pei Z, Li H, et al. Characterization of a Novel HIV-1 Circulating Recombinant Form (CRF80_0107) among men who have sex with men in China. AIDS Res Hum Retroviruses 2019; 35(4): 419-23.
[http://dx.doi.org/10.1089/aid.2018.0226] [PMID: 30259751]
[7]
Zhang C, Feng Y, Gao L, et al. Genetic characterization and recombinant history of a novel HIV-1 Circulating Recombinant Form (CRF101_01B) identified in Yunnan, China. Infect Genet Evol 2019; 73: 109-12.
[http://dx.doi.org/10.1016/j.meegid.2019.04.024] [PMID: 31028881]
[8]
Song Y, Feng Y, Miao Z, et al. Near-full-length genome sequences of a novel HIV-1 Circulating Recombinant Form, CRF01_AE/B′/C (CRF78_cpx), in Yunnan, China. AIDS Res Hum Retroviruses 2016; 32(6): 601-6.
[http://dx.doi.org/10.1089/aid.2015.0351] [PMID: 26885715]
[9]
Zhang W, Han X, An M, et al. Identification and characterization of a novel HIV-1 Circulating Recombinant Form (CRF59_01B) identified among men-who-have-sex-with-men in China. PLoS One 2014; 9(6): e99693.
[http://dx.doi.org/10.1371/journal.pone.0099693] [PMID: 24978029]
[10]
Li X, Ning C, He X, et al. Genome sequences of a novel HIV-1 Circulating Recombinant Form (CRF61_BC) identified among heterosexuals in China. Genome Announc 2013; 1(3): e00326-13.
[http://dx.doi.org/10.1128/genomeA.00326-13] [PMID: 23814029]
[11]
Zhou J, Lu X, Feng Y, et al. Genome sequence of a novel HIV-1 Circulating Recombinant Form (CRF103_01B) identified from Hebei Province, China. AIDS Res Hum Retroviruses 2020; 36(3): 234-41.
[http://dx.doi.org/10.1089/aid.2019.0196] [PMID: 31482718]
[12]
Han X, An M, Zhang W, et al. Genome sequences of a Novel HIV-1 Circulating Recombinant Form (CRF59_01B) identified among men who have sex with men in Northeastern China. Genome Announc 2013; 1(3): e00315-13.
[http://dx.doi.org/10.1128/genomeA.00315-13] [PMID: 23792741]
[13]
Wei H, His J, Feng Y, et al. Identification of a novel HIV-1 Circulating Recombinant Form (CRF62_BC) in western Yunnan of China. AIDS Res Hum Retroviruses 2014; 30(4): 380-3.
[http://dx.doi.org/10.1089/aid.2013.0235] [PMID: 24164474]
[14]
Li Y, Zhang Y, Wang H, et al. The first third-generation HIV-1 Circulating Recombinant Form (CRF114_0155) identified in central China. Arch Virol 2021; 166(12): 3409-16.
[http://dx.doi.org/10.1007/s00705-021-05213-9] [PMID: 34608524]
[15]
Feng Y, Gao Q, Gao L, et al. Identification of a newly emerging HIV-1 CRF111_01C comprising CRF01_AE and C in Yunnan, China: Genetic characterization and recombinant history. Infect Genet Evol 2021; 95: 105053.
[http://dx.doi.org/10.1016/j.meegid.2021.105053] [PMID: 34455089]
[16]
Song X, Zhou J, Li M, Feng Y, Xing H, Wei S. Near full-length genomic characterization of a novel HIV-1 Circulating Recombinant Form (CRF107_01B) identified among men who have sex with men in heilongjiang province of China. AIDS Res Hum Retroviruses 2021; 37(2): 151-6.
[http://dx.doi.org/10.1089/aid.2020.0159] [PMID: 32820943]
[17]
Miao J, Ran J, Song Y, et al. Characterization of a novel HIV-1 circulating recombinant form, CRF01_AE/B′/C (CRF96_cpx), in Yunnan, China. AIDS Res Hum Retroviruses 2018; 34(4): 393-7.
[http://dx.doi.org/10.1089/aid.2017.0288] [PMID: 29258320]
[18]
Zhou J, Li M, Min C, Ma Y, Shao Y, Xing H. Near full-length genomic characterization of a Novel HIV-1 Circulating Recombinant Form (CRF106_cpx) identified among heterosexuals in China. AIDS Res Hum Retroviruses 2020; 36(10): 875-80.
[http://dx.doi.org/10.1089/aid.2020.0101] [PMID: 32640836]
[19]
Wang X, Zhang Y, Liu Y, et al. Phylogenetic analysis of sequences in the HIV database revealed multiple potential circulating recombinant forms in China. AIDS Res Hum Retroviruses 2021; 37(9): 694-705.
[http://dx.doi.org/10.1089/aid.2020.0190] [PMID: 33390081]
[20]
Piyasirisilp S, McCutchan FE, Carr JK, et al. A recent outbreak of human immunodeficiency virus type 1 infection in southern China was initiated by two highly homogeneous, geographically separated strains, circulating recombinant form AE and a novel BC recombinant. J Virol 2000; 74(23): 11286-95.
[http://dx.doi.org/10.1128/JVI.74.23.11286-11295.2000] [PMID: 11070028]
[21]
Zhang L, Chen Z, Cao Y, et al. Molecular characterization of human immunodeficiency virus type 1 and hepatitis C virus in paid blood donors and injection drug users in china. J Virol 2004; 78(24): 13591-9.
[http://dx.doi.org/10.1128/JVI.78.24.13591-13599.2004] [PMID: 15564470]
[22]
He X, Xing H, Ruan Y, et al. A comprehensive mapping of HIV-1 genotypes in various risk groups and regions across China based on a nationwide molecular epidemiologic survey. PLoS One 2012; 7(10): e47289.
[http://dx.doi.org/10.1371/journal.pone.0047289] [PMID: 23056619]
[23]
Li Z, He X, Wang Z, et al. Tracing the origin and history of HIV-1 subtype B′ epidemic by near full-length genome analyses. AIDS 2012; 26(7): 877-84.
[http://dx.doi.org/10.1097/QAD.0b013e328351430d] [PMID: 22269972]
[24]
Li K, Liu M, Li J, et al. Genomic characterization of a novel HIV-1 second-Generation Recombinant Form (CRF01_AE/B) from men who have sex with men in guangxi zhuang autonomous Region, China. AIDS Res Hum Retroviruses 2019; 35(10): 972-7.
[http://dx.doi.org/10.1089/aid.2019.0149] [PMID: 31187643]
[25]
Ge Y, Liu Y, Fu G, et al. The molecular epidemiological and immunological characteristics of HIV-1 CRF01_AE/B recombinants in Nanjing, China. Front Microbiol 2022; 13: 936502.
[http://dx.doi.org/10.3389/fmicb.2022.936502] [PMID: 35910646]
[26]
Ou W, Li K, Feng Y, et al. Characterization of a new HIV-1 CRF01_AE/B recombinant virus form among men who have sex with men in Shanghai, China. AIDS Res Hum Retroviruses 2019; 35(4): 414-8.
[http://dx.doi.org/10.1089/aid.2018.0197] [PMID: 30229664]
[27]
Huang Q, Ou W, Feng Y, et al. Near full-length genomic characterization of HIV-1 CRF01_AE/B recombinant strains identified in Hebei, China. AIDS Res Hum Retroviruses 2019; 35(2): 196-204.
[http://dx.doi.org/10.1089/aid.2018.0130] [PMID: 30117324]
[28]
Ge Z, Getaneh Y, Liang Y, et al. Identification of a Novel HIV-1 Second-Generation (CRF01_AE/B) among men who have sex with men in Tianjin, China. AIDS Res Hum Retroviruses 2020; 36(2): 138-42.
[http://dx.doi.org/10.1089/aid.2019.0187] [PMID: 31482723]
[29]
Lu X, Zhang J, Wang Y, et al. Large transmission clusters of HIV-1 main genotypes among HIV-1 individuals before antiretroviral therapy in the Hebei Province, China. AIDS Res Hum Retroviruses 2020; 36(5): 427-33.
[http://dx.doi.org/10.1089/aid.2019.0199] [PMID: 31595767]
[30]
Shi P, Wang X, Fan W, et al. Pre-treatment drug resistance analysis of HIV-1 infected patients in Baoding City, 2019-2020. Chinese J Dermatovenereol 2021; 35(9): 1012-1016+1020..
[http://dx.doi.org/10.13735/j.cjdv.1001-7089.202103052]
[31]
Shi P, Chen Z, Meng J, et al. Molecular transmission networks and pre-treatment drug resistance among individuals with acute HIV-1 infection in Baoding, China. PLoS One 2021; 16(12): e0260670.
[http://dx.doi.org/10.1371/journal.pone.0260670] [PMID: 34855860]
[32]
Fan W, Su M, Meng J, et al. Characterization of a new HIV-1 CRF01_AE/B recombinant virus form among men who have sex with men in Baoding, Hebei, China. AIDS Res Hum Retroviruses 2022; 38(3): 237-41.
[http://dx.doi.org/10.1089/aid.2021.0209] [PMID: 35109712]
[33]
Li L, Chen L, Yang S, et al. Near full-length genomic characterization of a novel HIV type 1 subtype B/C recombinant strain from Yunnan, China. AIDS Res Hum Retroviruses 2010; 26(6): 711-6.
[http://dx.doi.org/10.1089/aid.2010.0001] [PMID: 20528150]
[34]
Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Mol Biol Evol 2013; 30(4): 772-80.
[http://dx.doi.org/10.1093/molbev/mst010] [PMID: 23329690]
[35]
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30(12): 2725-9.
[http://dx.doi.org/10.1093/molbev/mst197] [PMID: 24132122]
[36]
Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4(4): 406-25.
[37]
Zhou J, Xing H. Research progress on the prevalence of HIV recombinant strains in my country. Int J Virol 2019; 26(1): 66-8.
[38]
An M, Han X, Zhao B, et al. Cross-continental dispersal of major HIV-1 CRF01_AE Clusters in China. Front Microbiol 2020; 11: 61.
[http://dx.doi.org/10.3389/fmicb.2020.00061] [PMID: 32082287]
[39]
Wang X, He X, Zhong P, et al. Phylodynamics of major CRF01_AE epidemic clusters circulating in mainland of China. Sci Rep 2017; 7(1): 6330.
[http://dx.doi.org/10.1038/s41598-017-06573-6] [PMID: 28740095]
[40]
Lu X, Liu Y, Zhao H, et al. Recombinant patterns of nine novel HIV-1 recombinant strains identified in Hebei Province, China. AIDS Res Hum Retroviruses 2016; 32: 475-9.
[41]
Jia L, Li L, Gui T, et al. Analysis of HIV-1 intersubtype recombination breakpoints suggests region with high pairing probability may be a more fundamental factor than sequence similarity affecting HIV-1 recombination. Virol J 2016; 13(1): 156.
[http://dx.doi.org/10.1186/s12985-016-0616-1] [PMID: 27655081]
[42]
Obasa AE, Engelbrecht S, Jacobs GB. Near full-length HIV-1 subtype B sequences from the early South African epidemic, detecting a BD Unique Recombinant Form (URF) from a sample in 1985. Sci Rep 2019; 9(1): 6227.
[http://dx.doi.org/10.1038/s41598-019-42417-1] [PMID: 30996293]
[43]
Pessôa R, Loureiro P, Esther LM, et al. Ultra-deep sequencing of HIV-1 near full-length and partial proviral genomes reveals high genetic diversity among Brazilian blood donors. PLoS One 2016; 11(3): e0152499.
[http://dx.doi.org/10.1371/journal.pone.0152499] [PMID: 27031505]
[44]
Gan M, Zheng S, Hao J, et al. The prevalence of CRF55_01B among HIV-1 strain and its connection with traffic development in China. Emerg Microbes Infect 2021; 10(1): 256-65.
[http://dx.doi.org/10.1080/22221751.2021.1884004] [PMID: 33512306]
[45]
Zai J, Liu H, Lu Z, et al. Tracing the transmission dynamics of HIV-1 CRF55_01B. Sci Rep 2020; 10(1): 5098.
[http://dx.doi.org/10.1038/s41598-020-61870-x] [PMID: 32198405]
[46]
Wei L, Li H, Lv X, et al. Impact of HIV-1 CRF55_01B infection on the evolution of CD4 count and plasma HIV RNA load in men who have sex with men prior to antiretroviral therapy. Retrovirology 2021; 18(1): 22.
[http://dx.doi.org/10.1186/s12977-021-00567-z] [PMID: 34399785]
[47]
Liang B, Wei Q, Yang Y, et al. Identification of a novel HIV-1 CRF55_01B/B recombinant isolate in Guangxi, China. AIDS Res Hum Retroviruses 2020; 36(5): 434-9.
[http://dx.doi.org/10.1089/aid.2019.0222] [PMID: 31775514]
[48]
Han X, Takebe Y, Zhang W, et al. A Large-scale survey of CRF55_01B from men-who-have-sex-with-men in China: Implying the evolutionary history and public health impact. Sci Rep 2015; 5(1): 18147.
[http://dx.doi.org/10.1038/srep18147] [PMID: 26667846]
[49]
Zhao J, Cai W, Zheng C, et al. Origin and outbreak of HIV-1 CRF55_01B among MSM in Shenzhen, China. J Acquir Immune Defic Syndr 2014; 66(3): e65-7.
[http://dx.doi.org/10.1097/QAI.0000000000000144] [PMID: 24662297]
[50]
Han X, An M, Zhang W, et al. Genome sequences of a novel HIV-1 circulating recombinant form, CRF55_01B, identified in China. Genome Announc 2013; 1(1): e00050-12.
[http://dx.doi.org/10.1128/genomeA.00050-12] [PMID: 23405298]

Rights & Permissions Print Cite
Article Metrics
23
3
World Immunization Week
© 2024 Bentham Science Publishers | Privacy Policy