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Recent Advances in Drug Delivery and Formulation

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ISSN (Print): 2667-3878
ISSN (Online): 2667-3886

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Research Article

Doxorubicin Loaded Gold Nanoparticles Mitigate Liver Fibrosis and Inflammatory Cytokines Gene Expression in Rat

Author(s): Yasmeen Ishaq, Hamna Naeem, Sana Khurshid*, Rabia Tabbasam and Zeeshan Haider

Volume 16, Issue 4, 2022

Published on: 28 October, 2022

Page: [309 - 316] Pages: 8

DOI: 10.2174/2667387817666221020090050

Price: $65

Abstract

Background: Gold nanoparticles have the potential to be used as a carrier in drug delivery systems due to their small size, large surface area and short circulation time in blood.

Objective: This study demonstrates that doxorubicin conjugation with gold nanoparticles (AuNPs) may reduce its toxicity as well as improve therapeutic efficacy.

Methods: Five groups of Albino rats were used; 1: healthy control, 2: injured, 3: injured and treated with Dox, 4: injured and treated with AuNPs, 5: injured and treated with AuNPs: Dox. At the end of the experiment, blood and liver tissues were processed for biochemical and histopathological analysis. The expression of collagen, HO-1, IL-6 and TNF-α genes involved in liver fibrosis was observed through real-time PCR.

Results: At the end of the experiment, it was observed that the body weights of DOX-treated rats decreased by 0.72%, however, AuNPs and Au: DOX-treated rats were 15.3% and 29.13% respectively. The percentage of liver protection determined through alanine aminotransferase and aspartate aminotransferase levels in DOX, AuNPs and AuNPs: DOX treated groups were 39.21%, 79.26%, 98.17% and 47.77%, 84.17%, 97.92%, respectively, representing better recovering liver in Dox-AuNPs treated rats compared to others. Histopathological and gene expression studies further support the findings. The mRNA expression levels of inflammatory and oxidative stress-related genes HO-1, IL-6 and TNF- α were upregulated in the injured group but downregulated in the treated group.

Conclusion: As depicted through biochemical, histopathological and gene expression studies, Au: DOX conjugate group seems to be protective against liver fibrosis.

Keywords: Heam oxygenase, collagen, drug delivery, liver fibrosis, gene expression, toxicity.

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