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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Research Article

MicroRNA-137 Inhibits Esophageal Squamous Cell Carcinoma by Downregulating DAAM1

Author(s): Weina Li, Xiangdong Bai, Ruyuan Guo, Xiaolong Xing, Huanhu Zhang* and Xuezhen Gao*

Volume 29, Issue 10, 2022

Published on: 06 October, 2022

Page: [882 - 890] Pages: 9

DOI: 10.2174/0929866529666220819123149

Price: $65


Background: A growing body of evidence demonstrates that miR-137 acts against cancers; however, the biological function of miR-137 in esophageal squamous cell carcinoma (ESCC) remains to be fully understood.

Objective: The aim of this study is to explore the role of miR-137 in ESCC.

Methods: miR-137 expression was detected by reverse-transcription quantitative polymerase chain reaction (RT-qPCR), and target protein expression was detected by western blot. Cell counting, colony formation and flow cytometry were employed to determine the effects of miR-137 on the growth of ESCC cells. Dual-luciferase reporter assay was performed to validate the binding of miR- 137 with a dishevelled-associated activator of morphogenesis 1 (DAAM1) 3’-UTR.

Results: miR-137 was shown to be down-regulated in ESCC. miR-137 expression was inversely correlated with the 5-year survival rate of ESCC patients. Up-regulated miR-137 attenuated ESCC proliferation and promoted ESCC cell apoptosis. Meanwhile, to further reveal how miR-137 regulated the malignant behaviors of ESCC, the downstream mRNA binding targets of miR-137 were explored. miR-137 was demonstrated to bind DAAM1 3’-UTR and repressed the expression of DAAM1. The expression of DAAM1 and miR-137 in ESCC was inversely correlated. Additionally, the reintroduction of DAAM1 had the capacity to reverse the negative role of miR- 137 in ESCC cell growth.

Conclusion: These findings have uncovered the new function of miR-137 in ESCC via negatively regulating DAAM1, suggesting miR-137 as a potent therapeutic candidate for ESCC treatment.

Keywords: miR-137, ESCC, DAAM1, gene expression, squamous cell carcinoma, downregulating.

Graphical Abstract
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