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Current Nanomedicine

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ISSN (Print): 2468-1873
ISSN (Online): 2468-1881

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Research Article

Biogenic ZnO Nanoflowers: As an Alternative Antibacterial Nanomedicine

Author(s): Ravi Mani Tripathi*, Ramesh Namdeo Pudake, Braj Raj Shrivastav and Archana Shrivastav

Volume 12, Issue 1, 2022

Published on: 06 July, 2022

Page: [76 - 81] Pages: 6

DOI: 10.2174/2468187312666220408114236

Price: $65

Abstract

Background: Zinc oxide (ZnO) nanoparticles have been widely investigated for the development of next-generation nano-antibiotics against a broad range of microorganisms including multi-drug resistance. The morphology of nanomaterials plays an important role in antibacterial activity.

Objective: The research goal is focused on the development of a low-cost antibacterial agent.

Methods: The biosynthesis method was used to make ZnO nanoflowers. The antibacterial activity of these biogenic ZnO nanoflowers was analyzed by three methods: growth curve, well diffusion, and colony-forming unit count (CFU) assays.

Results: The assay methods used in this study confirmed the antibacterial activity of ZnO nanoflowers. The growth curve shows that 0.5 mg/mL concentration of ZnO nanoflowers acted as an effective bactericide as no significant optical absorption and virtually bacterial growth were observed. The inhibition zone was found at 25 mm at 70 μg of ZnO nanoflowers.

Conclusion: The unique, simplistic, environmental-friendly, and cost-effective biosynthesis method was established for the ZnO nanoflowers using biomass of Bacillus licheniformis. The resulted ZnO nanoflowers show excellent antibacterial activity which could be used as an alternative to antibiotics in therapeutic processes.

Keywords: Biosynthesis, ZnO nanoflowers, nanomaterials, antibacterial activity, growth curve, inhibition zone, colony forming unit.

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[1]
Tripathi RM, Park SH, Kim G, et al. A Metal-induced redshift of optical spectra of gold nanoparticles: An instant, sensitive, and selective visual detection of lead ions. Int Biodeterior Biodegradation 2019; 144: 104740.
[http://dx.doi.org/10.1016/j.ibiod.2019.104740]
[2]
Tripathi RM, Gupta RK, Singh P, et al. Ultra-sensitive detection of mercury (II) ions in water sample using gold nanoparticles synthesized by Trichoderma harzianum and their mechanistic approach. Sens Actuators B Chem 2014; 204: 637-46.
[http://dx.doi.org/10.1016/j.snb.2014.08.015]
[3]
Zahmouli N, Marini S, Guediri M, et al. Nanostructured nickel on the porous carbon-silica matrix as an efficient electrocatalytic material for a non-enzymatic glucose sensor. Chemosensors (Basel) 2018; 6(4): 54.
[http://dx.doi.org/10.3390/chemosensors6040054]
[4]
Shi LE, Li ZH, Zheng W, Zhao YF, Jin YF, Tang ZX. Synthesis, antibacterial activity, antibacterial mechanism and food applications of ZnO nanoparticles: A review. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2014; 31(2): 173-86.
[http://dx.doi.org/10.1080/19440049.2013.865147] [PMID: 24219062]
[5]
Tripathi RM, Gupta RK, Shrivastav A, Singh MP, Shrivastav BR, Singh P. Trichoderma koningii assisted biogenic synthesis of silver nanoparticles and evaluation of their antibacterial activity. Adv Nat Sci: Nanosci Nanotechnol 2013; 4(3): 035005.
[http://dx.doi.org/10.1088/2043-6262/4/3/035005]
[6]
Tripathi RM, Shrivastav A, Shrivastav BR. Biogenic gold nanoparticles: As a potential candidate for brain tumor directed drug delivery. Artif Cells Nanomed Biotechnol 2015; 43(5): 311-7.
[http://dx.doi.org/10.3109/21691401.2014.885445] [PMID: 24588231]
[7]
Mehrotra N, Tripathi RM. Short interfering RNA therapeutics: Nanocarriers, prospects, and limitations. IET Nanobiotechnol 2015; 9(6): 386-95.
[http://dx.doi.org/10.1049/iet-nbt.2015.0018]
[8]
Tripathi RM, Chung SJ. Phytosynthesis of palladium nanoclusters: An efficient nanozyme for ultrasensitive and selective detection of reactive oxygen species. Molecules 2020; 25(15): 3349.
[http://dx.doi.org/10.3390/molecules25153349] [PMID: 32717976]
[9]
Tripathi RM, Chung SJ. Eco-friendly synthesis of SnO2-Cu nanocomposites and evaluation of their peroxidase mimetic activity. Nanomaterials (Basel) 2021; 11(7): 1798.
[http://dx.doi.org/10.3390/nano11071798] [PMID: 34361185]
[10]
Saxena A, Tripathi RM, Zafar F, Singh P. Green synthesis of silver nanoparticles using aqueous solution of Ficus benghalensis leaf extract and characterization of their antibacterial activity. Mater Lett 2012; 67(1): 91-4.
[http://dx.doi.org/10.1016/j.matlet.2011.09.038]
[11]
Ganesan V, Hariram M, Vivekanandhan S, Muthuramkumar S. Periconium sp.(endophytic fungi) extract mediated sol-gel synthesis of ZnO nanoparticles for antimicrobial and antioxidant applications. Mater Sci Semicond Process 2020; 105: 104739.
[http://dx.doi.org/10.1016/j.mssp.2019.104739]
[12]
Tripathi RM, Bhadwal AS, Gupta RK, Singh P, Shrivastav A, Shrivastav BR. ZnO nanoflowers: Novel biogenic synthesis and enhanced photocatalytic activity. J Photochem Photobiol B 2014; 141: 288-95.
[http://dx.doi.org/10.1016/j.jphotobiol.2014.10.001] [PMID: 25463680]
[13]
Mitra S, Chandra S, Laha D, et al. Unique chemical grafting of carbon nanoparticle on fabricated ZnO nanorod: Antibacterial and bioimaging property. Mater Res Bull 2012; 47(3): 586-94.
[http://dx.doi.org/10.1016/j.materresbull.2011.12.036]
[14]
Auer G, Griebler WD, Jahn B. Industrial inorganic pigments. In: Buxbaum G, Pfaff G, Eds. Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA 2005; pp. 129-30.
[15]
Heideman G, Noordermeer JW, Datta RN, van Baarle B. Various ways to reduce zinc oxide levels in S‐SBR rubber compounds. In: Macromolecular Symposia. Weinheim: WILEY‐VCH Verlag 2006; 245: pp. (1)657-67.
[http://dx.doi.org/10.1002/masy.200651393]
[16]
Patnaik P. Handbook of inorganic chemicals. New York: McGraw-Hill 2003.
[17]
Araujo-Lima CF, Nunes RJ, Carpes RM, Aiub CA, Felzenszwalb I. Pharmacokinetic and toxicological evaluation of a zinc gluconate-based chemical sterilant using in vitro and in silico approaches. BioMed Res Int 2017; 2017: 5746768.
[http://dx.doi.org/10.1155/2017/5746768] [PMID: 28197414]
[18]
Greenberg MI, Vearrier D. Metal fume fever and polymer fume fever. Clin Toxicol (Phila) 2015; 53(4): 195-203.
[http://dx.doi.org/10.3109/15563650.2015.1013548] [PMID: 25706449]
[19]
Ranjithkumar B, Ramalingam HB, Kumar ER, et al. Natural fuels (Honey and Cow urine) assisted combustion synthesis of zinc oxide nanoparticles for antimicrobial activities. Ceram Int 2021; 47(10): 14475-81.
[http://dx.doi.org/10.1016/j.ceramint.2021.02.026]
[20]
Senthilkumar N, Nandhakumar E, Priya P, Soni D, Vimalan M, Potheher IV. Synthesis of ZnO nanoparticles using leaf extract of Tectona grandis (L.) and their anti-bacterial, anti-arthritic, anti-oxidant and in vitro cytotoxicity activities. New J Chem 2017; 41(18): 10347-56.
[http://dx.doi.org/10.1039/C7NJ02664A]
[21]
Wu CL, Chang L, Chen HG, Lin CW, Chao YC, Yan JK. Growth and characterization of chemical-vapor-deposited zinc oxide nanorods. Thin Solid Films 2006; 498(1-2): 137-41.
[http://dx.doi.org/10.1016/j.tsf.2005.07.096]
[22]
Musić S, Šarić A, Popović S. Formation of nanosize ZnO particles by thermal decomposition of zinc acetylacetonate monohydrate. Ceram Int 2010; 36(3): 1117-23.
[http://dx.doi.org/10.1016/j.ceramint.2009.12.008]
[23]
Rani S, Suri P, Shishodia PK, Mehra RM. Synthesis of nanocrystalline ZnO powder via sol-gel route for dye-sensitized solar cells. Sol Energy Mater Sol Cells 2008; 92(12): 1639-45.
[http://dx.doi.org/10.1016/j.solmat.2008.07.015]
[24]
Tripathi RM, Chung SJ. Biogenic nanomaterials: Synthesis, characterization, growth mechanism, and biomedical applications. J Microbiol Methods 2019; 157: 65-80.
[http://dx.doi.org/10.1016/j.mimet.2018.12.008] [PMID: 30552971]
[25]
Tripathi RM, Yoon SY, Ahn D, Chung SJ. Facile synthesis of triangular and hexagonal anionic gold nanoparticles and evaluation of their cytotoxicity. Nanomaterials (Basel) 2019; 9(12): 1774.
[http://dx.doi.org/10.3390/nano9121774] [PMID: 31842495]
[26]
Rao RP, Mishra S, Tripathi RM, Jain SK. Bismuth oxide nanorods: Phytochemical mediated one-pot synthesis and growth mechanism. Inorg Nano-Met Chem 2021; 15: 1-8.
[http://dx.doi.org/10.1080/24701556.2021.1980037]
[27]
Mukherjee P, Roy M, Mandal BP, et al. Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T. asperellum. Nanotechnology 2008; 19(7): 075103.
[http://dx.doi.org/10.1088/0957-4484/19/7/075103] [PMID: 21817628]
[28]
Vimala K, Sundarraj S, Paulpandi M, Vengatesan S, Kannan S. Green synthesized doxorubicin- loaded zinc oxide nanoparticles regulates the Bax and Bcl-2 expression in breast and colon carcinoma. Process Biochem 2014; 49(1): 160-72.
[http://dx.doi.org/10.1016/j.procbio.2013.10.007]
[29]
Venkatachalam P, Jayaraj M, Manikandan R, et al. Zinc oxide nanoparticles (ZnONPs) alleviate heavy metal-induced toxicity in Leucaena leucocephala seedlings: A physiochemical analysis. Plant Physiol Biochem 2017; 110: 59-69.
[http://dx.doi.org/10.1016/j.plaphy.2016.08.022] [PMID: 27622846]
[30]
Droepenu EK, Asare EA, Wee BS, Wahi RB, Ayertey F, Kyene MO. Biosynthesis, characterization, and antibacterial activity of ZnO nanoaggregates using an aqueous extract from Anacardium occidentale leaf: Comparative study of different precursors. Beni Suef Univ J Basic Appl Sci 2021; 10(1): 1-0.
[http://dx.doi.org/10.1186/s43088-020-00091-7]
[31]
Jain D, Shivani Bhojiya AA, et al. Microbial fabrication of zinc oxide nanoparticles and evaluation of their antimicrobial and photocatalytic properties. Front Chem 2020; 8: 778.
[http://dx.doi.org/10.3389/fchem.2020.00778] [PMID: 33195020]
[32]
Selvarajan E, Mohanasrinivasan V. Biosynthesis and characterization of ZnO nanoparticles using Lactobacillus Plantarum VITES07. Mater Lett 2013; 112: 180-2.
[http://dx.doi.org/10.1016/j.matlet.2013.09.020]

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