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Cardiovascular & Hematological Agents in Medicinal Chemistry


ISSN (Print): 1871-5257
ISSN (Online): 1875-6182

Research Article

Artocarpus Altilis (Breadfruit) could Reverse Myocardial Infarction Through the Normalization of the Oxygen Haemoglobin Dissociation Curve

Author(s): Jemesha Thomas, Tanya Anderson, Tameika James-Green, Magdalene Nwokocha, Javier Palacios, Dagogo Pepple and Chukwuemeka Nwokocha*

Volume 20, Issue 3, 2022

Published on: 26 April, 2022

Page: [212 - 218] Pages: 7

DOI: 10.2174/1871525720666220203110919

Price: $65


Objective: The study was performed to assess if hematological mechanisms such as blood flow modulation, P50 and Oxygen haemoglobin dissociation are involved in Artocarpus altilis leaf extract amelioration of Isoproterenol-Induced Myocardial Damage in rats.

Methods: Twenty (20) adult male Sprague-Dawley rats were randomly divided into 5 groups. Group 1 served as the control, group 3 and 5 received 50 and 100 mg/kg Artocarpus altilis water extract, respectively, after being induced with Isoproterenol twice (85 mg/ kg) at a 24-hour period. Group 2 received 85mg/kg isoproterenol only, while group 4 received 50 mg/kg Artocarpus altilis extract only for 6 days. The Hematological parameters were assessed using an automatic Coulter Counter, blood flow was assessed with the CODA machine using the tail cuff method, while blood viscosity was measured at native hematocrit and the oxygen haemoglobin dissociation curves were assessed with the BioProfiler and Hemox Analyzer at the end of seven days.

Results: Artocarpus altilis treatment ameliorated the ISO induced increases in viscosity, increased the ISO induced decreased blood flow and influenced oxygen release through its effects on the P50 of the oxygen hemoglobin dissociation curve, AA treatment also reversed the ISO induced weight loss. Apart from the changes in MCH, MCV, there were no significant differences in hematological parameters.

Conclusion: This study reported the effects of Artocarpus atilis on the improvement of oxygen availability, the reduction of blood viscosity, and the improvement of blood flow through its influence on endothelial functions and NO availability. Our study further highlights The usefulness of A.atilis, as having a beneficial cardiovascular and haematological outcome in experimental myocardial infarction and as such, potential drug discovery for diseases of cardiovascular & hematological involvement.

Keywords: Artocarpus altilis, isoproterenol, oxygen hemoglobin curve, hematology, viscosity, myocardial infarction.

Graphical Abstract
Rathore, N.; John, S.; Kale, M.; Bhatnagar, D. Lipid peroxidation and antioxidant enzymes in isoproterenol induced oxidative stress in rat tissues. Pharmacol. Res., 1998, 38(4), 297-303.
[] [PMID: 9774493]
Geng, B.; Chang, L.; Pan, C.; Qi, Y.; Zhao, J.; Pang, Y.; Du, J.; Tang, C. Endogenous hydrogen sulfide regulation of myocardial injury induced by isoproterenol. Biochem. Biophys. Res. Commun., 2004, 318(3), 756-763.
Aman, U.; Vaibhav, P.; Balaraman, R. Tomato lycopene attenuates myocardial infarction induced by isoproterenol: Electrocardiographic, biochemical and anti-apoptotic study. Asian Pac. J. Trop. Biomed., 2012, 2(5), 345-351.
[] [PMID: 23569928]
Branco, A.F.; Pereira, S.L.; Moreira, A.C.; Holy, J.; Sardão, V.A.; Oliveira, P.J. Isoproterenol cytotoxicity is dependent on the differentia-tion state of the cardiomyoblast H9c2 cell line. Cardiovasc. Toxicol., 2011, 11(3), 191-203.
[] [PMID: 21455642]
Nwokocha, C.; Palacios, J.; Simirgiotis, M.J.; Thomas, J.; Nwokocha, M.; Young, L.; Thompson, R.; Cifuentes, F.; Paredes, A.; Delgoda, R. Aqueous extract from leaf of Artocarpus altilis provides cardio-protection from isoproterenol induced myocardial damage in rats: Nega-tive chronotropic and inotropic effects. J. Ethnopharmacol., 2017, 203, 163-170.
[] [PMID: 28342858]
Nwokocha, C.R.; Warren, I.; Palacios, J.; Simirgiotis, M.; Nwokocha, M.; Harrison, S.; Thompson, R.; Paredes, A.; Bórquez, J.; Lavado, A.; Cifuentes, F. Modulatory effect of guinep (Melicoccus bijugatus jacq) fruit pulp extract on isoproterenol-induced myocardial damage in rats. Identification of major metabolites using high resolution UHPLC Q-orbitrap mass spectrometry. Molecules, 2019, 24(2), 235.
[] [PMID: 30634603]
Nwokocha, C.R.; Gordon, A.; Palacios, J.; Paredes, A.; Cifuentes, F.; Francis, S.; Watson, J.; Delgoda, R.; Nwokocha, M.; Alexander-Lindo, R.; Thompson, R.; Minott-Kates, D.; Yakubu, M.A. Hypotensive and antihypertensive effects of an aqueous extract from guinep fruit (Melicoccus bijugatus jacq) in rats. Sci. Rep., 2020, 10(1), 18623.
[] [PMID: 33122667]
Günther, T.; Vormann, J.; Höllriegl, V. Isoproterenol-induced Mg2+ uptake in liver. FEBS Lett., 1992, 307(3), 333-336.
[] [PMID: 1322836]
Ghosh, P.M.; Shu, Z.J.; Zhu, B.; Lu, Z.; Ikeno, Y.; Barnes, J.L.; Yeh, C.K.; Zhang, B.X.; Katz, M.S.; Kamat, A. Role of β-adrenergic recep-tors in regulation of hepatic fat accumulation during aging. J. Endocrinol., 213(3), 251-261.
[] [PMID: 22457517]
Neri, M.; Cerretani, D.; Fiaschi, A.I.; Laghi, P.F.; Lazzerini, P.E.; Maffione, A.B.; Micheli, L.; Bruni, G.; Nencini, C.; Giorgi, G.; D’Errico, S.; Fiore, C.; Pomara, C.; Riezzo, I.; Turillazzi, E.; Fineschi, V. Correlation between cardiac oxidative stress and myocardial pathology due to acute and chronic norepinephrine administration in rats. J. Cell. Mol. Med., 2007, 11(1), 156-170.
[] [PMID: 17367510]
Chattopadhyay, A.; Biswas, S.; Bandyopadhyay, D.; Sarkar, C.; Datta, A.G. Effect of isoproterenol on lipid peroxidation and antioxidant enzymes of myocardial tissue of mice and protection by quinidine. Mol. Cell. Biochem., 2003, 245(1-2), 43-49.
[] [PMID: 12708743]
Allen, J.E.; Rasmussen, H. Human red blood cells: Prostaglandin E2, epinephrine, and isoproterenol alter deformability. Human red blood cells: prostaglandin E2, epinephrine, and isoproterenol alter deformability. Science, 1971, 174(4008), 512-514.
[] [PMID: 5110430]
Ybañez-Julca, R.O.; Asunción-Alvarez, D.; Quispe-Díaz, I.M.; Palacios, J.; Bórquez, J.; Simirgiotis, M.J.; Perveen, S.; Nwokocha, C.R.; Cifuentes, F.; Paredes, A. Metabolomic profiling of mango (Mangifera indicaLinn) leaf extract and its intestinal protective effect and anti-oxidant activity in different biological models. Molecules, 2020, 25(21), 5149.
[] [PMID: 33167456]
Nwokocha, C.R.; Owu, D.U.; McLaren, M.; Murray, J.; Delgoda, R.; Thaxter, K.; McCalla, G.; Young, L. Possible mechanisms of action of the aqueous extract of Artocarpus altilis (breadfruit) leaves in producing hypotension in normotensive Sprague-Dawley rats. Pharm. Biol., 2012, 50(9), 1096-1102.
[] [PMID: 22830437]
Cifuentes, F.; Paredes, A.; Palacios, J.; Muñoz, F.; Carvajal, L.; Nwokocha, C.R.; Morales, G. Hypotensive and antihypertensive effects of a hydroalcoholic extract from Senecio nutans Sch. Bip. (Compositae) in mice: Chronotropic and negative inotropic effect, a nifedipine-like action. J. Ethnopharmacol., 2016, 179(179), 367-374.
[] [PMID: 26724424]
Paredes, A.; Palacios, J.; Quispe, C.; Nwokocha, C.R.; Morales, G.; Kuzmicic, J.; Cifuentes, F. Hydroalcoholic extract and pure com-pounds from Senecio nutans Sch. Bip (Compositae) induce vasodilation in rat aorta through endothelium-dependent and independent mechanisms. J. Ethnopharmacol., 2016, 192(192), 99-107.
[] [PMID: 27392595]
Facey, J.; Young, L.; Nwokocha, C. Relaxation responses of ketamine and propofol to vasoactive agents in streptozotocin-induced diabetic rats. Niger. J. Physiol. Sci., 2020, 35(1), 33-39.
[PMID: 33084625]
Francis, S.; Laurieri, N.; Nwokocha, C.; Delgoda, R. Treatment of rats with apocynin has considerable inhibitory effects on arylamine N-acetyltransferase activity in the liver. Sci. Rep., 2016, 6(1), 26906.
[] [PMID: 27242013]
Nwokocha, C.R.; Owu, D.U.; Nwokocha, M.I.; Ufearo, C.S.; Iwuala, M.O. Comparative study on the hepatoprotection to heavy metals of Zingiber officinale. Pharmacognosy Res., 2012, 4(4), 208-213.
[] [PMID: 23225964]
Hagley, A.C.; Younger-Coleman, N.O.; Richards, A.A.; Nwokocha, C.R.; Pepple, D.J. The effect of sildenafil on the elasticity of erythro-cytes in homozygous sickle cell disease. Indian J. Med. Sci., 2011, 65(6), 243-249.
[] [PMID: 23391833]
Ellis, S.S.; Pepple, D.J. Sildenafil increases the p50 and shifts the oxygen-hemoglobin dissociation curve to the right. J. Sex. Med., 2015, 12(12), 2229-2232.
[] [PMID: 26553865]
McCalla, G.; Brown, P.D.; Cole, W.C.; Campbell, C.; Nwokocha, C.R. Cadmium-induced hypertension is associated with renal myosin light chain phosphatase inhibition via increased T697 phosphorylation and p44 mitogen-activated protein kinase levels. Hypertens. Res., 2021, 44(8), 941-954.
[] [PMID: 33972751]
Filho, H.; Nestor, L.F.; Bezerra de Sousa, R.; Rebouças de Carvalho, E.; Lobo, P.; Filho, J. Experimental model of myocardial infarction induced by isoproterenol in rats. Rev. Bras. Cir. Cardiovasc., 2011, 26(3), 469-476.
[] [PMID: 22086586]
Pabisiak, A.; Bromboszcz, J.; Kmiec, S.; Dendura, M.; Dabrowski, Z.; Smolenski, O. Changes in the complete blood count and blood rhe-ology in patients after myocardial infarction participating in the rehabilitation programme. Clin. Hemorheol. Microcirc., 2015, 61(3), 541-547.
[] [PMID: 26410862]
Caimi, G.; Hopps, E.; Montana, M.; Andolina, G.; Urso, C.; Canino, B.; Lo Presti, R. Analysis of the blood viscosity behavior in the sicili-an study on juvenile myocardial infarction. Clin. Appl. Thromb. Hemost., 2018, 24(8), 1276-1281.
[] [PMID: 29792062]
Jannesar, K; Abbaszadeh, S; Malekinejad, H; Soraya, H Cardioprotective effects of memantine in myocardial ischemia: Ex vivo and in vivo studies. Eur J Pharmacol, 2020, 5, 882:173277.
Beshel, J.A.; Palacios, J.; Beshel, F.N.; Nku, C.O.; Owu, D.U.; Nwokocha, M.; Bórquez, J.; Simirgiotis, M.J.; Nwokocha, C.R. Blood pressure-reducing activity of Gongronema latifolium benth. (Apocynaeceae) and the identification of its main phytochemicals by UHPLC Q-Orbitrap mass spectrometry. J. Basic Clin. Physiol. Pharmacol., 2019, 31(1)
Garg, M.; Khanna, D. Exploration of pharmacological interventions to prevent isoproterenol-induced myocardial infarction in experi-mental models. Ther. Adv. Cardiovasc. Dis., 2014, 8(4), 155-169.
[] [PMID: 24817146]
Nwokocha, C.R.; Ajayi, I.O.; Owu, D.U.; Ebeigbe, A.B. Specificity of vascular reactivity and altered response in experimental malaria. West Indian Med. J., 2011, 60(3), 330-335.
[PMID: 22224347]
Nwokocha, C.R.; Ajayi, I.O.; Ebeigbe, A.B. Altered vascular reactivity induced by malaria parasites. West Indian Med. J., 2011, 60(1), 13-18.
[PMID: 21809705]
Nwokocha, C.R.; Palacios, J.; Rattray, V.R.; McCalla, G.; Nwokocha, M.; McGrowder, D. Protective effects of apocynin against cadmium toxicity and serum parameters; evidence of a cardio-protective influence. Inorg. Chim. Acta, 2020, 503, 119411.
Nwokocha, C.R.; Ozolua, R.I.; Owu, D.U.; Nwokocha, M.I.; Ugwu, A.C. Antihypertensive properties of Allium sativum (garlic) on normoten-sive and two kidney one clip hypertensive rats. Niger. J. Physiol. Sci., 2011, 26(2), 213-218.
[PMID: 22547193]

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