Abstract
Advances in recombinant DNA biotechnology have stimulated demand for the development of protein formulations within the pharmaceutical industry. A major challenge in preparation of protein formulations is their often poor stability during formulation processing and storage. Combinations of analytical techniques that probe different aspects of protein structure are often required to ensure that higher order (secondary and tertiary) structure and biological activity are maintained. Vibrational spectroscopy has received increasing attention as it offers effective, reliable, fast, simple and non-destructive measurement of protein structure. This review describes recent applications of near-infrared, infrared and Raman spectroscopy for protein analysis. This paper is intended to serve as guidance for researchers beginning in spectral analysis. It is hoped that the information herein will encourage and facilitate research associated with the use of these spectroscopic techniques in protein pharmaceutics.
Keywords: Infrared spectroscopy, Raman spectroscopy, Near infrared spectroscopy, Protein
Current Pharmaceutical Analysis
Title: Insights into the Structure of Protein by Vibrational Spectroscopy
Volume: 4 Issue: 2
Author(s): Ruedeeporn Tantipolphan, Thomas Rades and Natalie J. Medlicott
Affiliation:
Keywords: Infrared spectroscopy, Raman spectroscopy, Near infrared spectroscopy, Protein
Abstract: Advances in recombinant DNA biotechnology have stimulated demand for the development of protein formulations within the pharmaceutical industry. A major challenge in preparation of protein formulations is their often poor stability during formulation processing and storage. Combinations of analytical techniques that probe different aspects of protein structure are often required to ensure that higher order (secondary and tertiary) structure and biological activity are maintained. Vibrational spectroscopy has received increasing attention as it offers effective, reliable, fast, simple and non-destructive measurement of protein structure. This review describes recent applications of near-infrared, infrared and Raman spectroscopy for protein analysis. This paper is intended to serve as guidance for researchers beginning in spectral analysis. It is hoped that the information herein will encourage and facilitate research associated with the use of these spectroscopic techniques in protein pharmaceutics.
Export Options
About this article
Cite this article as:
Tantipolphan Ruedeeporn, Rades Thomas and Medlicott J. Natalie, Insights into the Structure of Protein by Vibrational Spectroscopy, Current Pharmaceutical Analysis 2008; 4 (2) . https://dx.doi.org/10.2174/157341208784246314
DOI https://dx.doi.org/10.2174/157341208784246314 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Hypertension and Atrial Fibrillation: Any Change with the New Anticoagulants
Current Pharmaceutical Design Curcumin, an Active Constiuent of the Ancient Medicinal Herb Curcuma longa L.: Some Uses and the Establishment and Biological Basis of Medical Efficacy
CNS & Neurological Disorders - Drug Targets Vasculitis Beyond the Kidneys
Clinical Immunology, Endocrine & Metabolic Drugs (Discontinued) Physicochemical and Pharmacological Characterization of Etodolac- Loaded Poloxamer Gel as a Rectal Delivery System
Letters in Drug Design & Discovery Animal Models of Xenobiotic Receptors
Current Drug Metabolism Inflammation in Depression and the Potential for Anti-Inflammatory Treatment
Current Neuropharmacology Cruzipain, the Major Cysteine Protease of Trypanosoma cruzi: A Sulfated Glycoprotein Antigen as Relevant Candidate for Vaccine Development and Drug Target. A Review
Current Medicinal Chemistry Cancer Vaccines for Hormone/Growth Factor Immune Deprivation:A Feasible Approach for Cancer Treatment
Current Cancer Drug Targets Ethosomes and its Applications in Transdermal Drug Delivery
Current Drug Therapy CYP24A1 as a Potential Target for Cancer Therapy
Anti-Cancer Agents in Medicinal Chemistry Unique Applications of Novel Antifungal Drug Combinations
Anti-Infective Agents in Medicinal Chemistry Role of Advanced Glycation End Products (AGEs) in Thrombogenic Abnormalities in Diabetes
Current Neurovascular Research Alcoholic Liver Disease: from CYP2E1 to CYP2A5
Current Molecular Pharmacology Herb Medicines against Osteoporosis: Active Compounds & Relevant Biological Mechanisms
Current Topics in Medicinal Chemistry A Review on the Pharmacology and Toxicology of Steviol Glycosides Extracted from Stevia rebaudiana
Current Pharmaceutical Design Monoaminergic Receptors as Modulators of the Perivascular Sympathetic and Sensory CGRPergic Outflows
Current Neuropharmacology Cell Encapsulation in Mammal Reproduction
Recent Patents on Drug Delivery & Formulation Human Amnion–Derived Cells as a Reliable Source of Stem Cells
Current Molecular Medicine The Role of Sympathetic Nervous System in the Progression of Chronic Kidney Disease in the Era of Catheter Based Sympathetic Renal Denervation
Current Clinical Pharmacology Dangerous Liaisons: Tau Interaction with Muscarinic Receptors
Current Alzheimer Research