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Current Pharmaceutical Biotechnology


ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Review Article

Diagnostic Accuracy and Validity of Serological and Molecular Tests for Hepatitis B and C

Author(s): Moein Zangiabadian, Amir Zamani, Mohammad Javad Nasiri, Elham Behzadi and Abbas Ali Imani Fooladi*

Volume 23, Issue 6, 2022

Published on: 19 July, 2021

Page: [803 - 817] Pages: 15

DOI: 10.2174/1389201022666210719162802

Price: $65


Introduction: Hepatitis B and C viruses are one of the leading causes of health problems in the world and early diagnosis and treatment of them are very important. Thereby, this study aimed to evaluate the validity and reliability of usable diagnostic tests for the detection of hepatitis B and C viruses in the clinical setting and to compare them with each other.

Materials and Methods: In this review article, we have searched major online databases, including PubMed and EMBASE. 42 retrieved articles were published between January 2000 and January 2020, which are summarized in this review.

Results: Immunoassay approaches are general techniques for the identification of pathogenic agents, among which ELISA is the gold standard for the detection of HBsAg. While serological techniques are not conclusive, molecular assays are really important because of the high sensitivity to detect chronic hepatitis B without HBeAg, in which viral loads are very low. Biosensors have more elevated selectivity and sensitivity and faster responses compared to other methods.

Conclusion: This study suggests that all of the molecular, serological, and biotechnological assays have advantages and disadvantages for diagnosing hepatitis B and C viruses which are dependent on the condition, so we should choose one of them in regards to the time, cost, and laboratory equipment along with the clinical symptoms.

Keywords: Hepatitis B virus, hepatitis C virus, diagnostic tests, serological assays, rapid Point-of-care test, molecular methods.

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