Abstract
Background: Alpha-terpineol is monoterpene alcohol with anti-tumor activity against different tumor cell lines (lung, breast, leukemias and colorectal) through blockage of NF-kB expression, which play an important role in tumor cells growth.
Objective: Evaluate the antitumor activity of alpha-terpineol in murine Sarcoma 180 cell line.
Methods: For the tests, different cytotoxic and genotoxic assays were used, including Trypan blue, cytokinesis- blocked micronucleus assay, comet assay, agarose gel DNA fragmentation, flow cytometry and cell viability using fluorescence. Ascitic fluid cells from sarcoma 180 were obtained from Mus musculus peritoneal cavity and Alpha-terpineol was tested at 100, 250 and 500 μg/mL. Doxorubicin and Cisplatin were used as positive controls.
Results: Cytotoxic effects of alpha-terpineol were found in all concentrations tested, reducing cell viability in 50.9; 38.53; 30.82% at 100, 250 and 500 μg/mL, respectively. Alpha-terpineol induced genotoxic effects due to DNA fragmentation (increased frequency and index of damage), and was clastogenic by increased micronuclei formation, nucleoplasmic bridges and nuclear buds. DNA fragmentation and increased cell death indicated that alpha-terpineol can cause early, late, and necrotic apoptosis.
Conclusion: Our data indicate that alpha-terpineol has antitumor activity revealed by cytogenetic mechanisms and / or loss of cell membrane integrity.
Keywords: Natural products, essential oils, monoterpene, alpha-terpineol, genotoxicity, sarcoma 180.
Anti-Cancer Agents in Medicinal Chemistry
Title:Alpha-Terpineol as Antitumor Candidate in Pre-Clinical Studies
Volume: 21 Issue: 15
Author(s): Helber A. Negreiros, Kariely Gonçalves de Moura, Maria L.L. Barreto do Nascimento, Débora Caroline do Nascimento Rodrigues, Paulo M.P. Ferreir, Débora C. Braz, Marlene Gomes de Farias, Layde de Sousa Corrêia, Ana R.S. Pereira, Lubna K.B. Santos, Juan C.R. Gonçalves, Anderson N. Mendes, Felipe C. Carneiro da Silva, Ana A.C.M. Cavalcant and Joáo Marcelo de Castro e Sousa*
Affiliation:
- Toxicological Genetics Research Laboratory (LAPGENIC), Postgraduate Program in Pharmaceutical Sciences, Federal University of Piaui, Teresina, Piaui,Brazil
Keywords: Natural products, essential oils, monoterpene, alpha-terpineol, genotoxicity, sarcoma 180.
Abstract:
Background: Alpha-terpineol is monoterpene alcohol with anti-tumor activity against different tumor cell lines (lung, breast, leukemias and colorectal) through blockage of NF-kB expression, which play an important role in tumor cells growth.
Objective: Evaluate the antitumor activity of alpha-terpineol in murine Sarcoma 180 cell line.
Methods: For the tests, different cytotoxic and genotoxic assays were used, including Trypan blue, cytokinesis- blocked micronucleus assay, comet assay, agarose gel DNA fragmentation, flow cytometry and cell viability using fluorescence. Ascitic fluid cells from sarcoma 180 were obtained from Mus musculus peritoneal cavity and Alpha-terpineol was tested at 100, 250 and 500 μg/mL. Doxorubicin and Cisplatin were used as positive controls.
Results: Cytotoxic effects of alpha-terpineol were found in all concentrations tested, reducing cell viability in 50.9; 38.53; 30.82% at 100, 250 and 500 μg/mL, respectively. Alpha-terpineol induced genotoxic effects due to DNA fragmentation (increased frequency and index of damage), and was clastogenic by increased micronuclei formation, nucleoplasmic bridges and nuclear buds. DNA fragmentation and increased cell death indicated that alpha-terpineol can cause early, late, and necrotic apoptosis.
Conclusion: Our data indicate that alpha-terpineol has antitumor activity revealed by cytogenetic mechanisms and / or loss of cell membrane integrity.
Export Options
About this article
Cite this article as:
Negreiros A. Helber , de Moura Gonçalves Kariely , Barreto do Nascimento L.L. Maria , do Nascimento Rodrigues Caroline Débora , Ferreir M.P. Paulo , Braz C. Débora , de Farias Gomes Marlene , de Sousa Corrêia Layde , Pereira R.S. Ana , Santos K.B. Lubna , Gonçalves C.R. Juan , Mendes N. Anderson , Carneiro da Silva C. Felipe , Cavalcant A.C.M. Ana and de Castro e Sousa Marcelo Joáo *, Alpha-Terpineol as Antitumor Candidate in Pre-Clinical Studies, Anti-Cancer Agents in Medicinal Chemistry 2021; 21 (15) . https://dx.doi.org/10.2174/1871520621999210104195820
DOI https://dx.doi.org/10.2174/1871520621999210104195820 |
Print ISSN 1871-5206 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5992 |
Call for Papers in Thematic Issues
Induction of cell death in cancer cells by modulating telomerase activity using small molecule drugs
Telomeres are distinctive but short stretches present at the corners of chromosomes and aid in stabilizing chromosomal makeup. Resynthesis of telomeres supported by the activity of reverse transcriptase ribonucleoprotein complex telomerase. There is no any telomerase activity in human somatic cells, but the stem cells and germ cells undergone telomerase ...read more
Role of natural compounds as anti anti-cancer agents
Cancer is considered the leading cause of worldwide mortality, accounting for nearly 10 million deaths in 2022. Cancer outcome can be improved through an appropriate screening and early detection and through an efficient clinical treatment. Chemotherapy remains an important approach in treatment o f several types of cancers, even though ...read more
Signaling and enzymatic modulators in cancer treatment
Cancer accounts for nearly 10 million deaths in 2022 and is considered the leading cause of worldwide mortality. Cancer outcome can be improved through an appropriate screening and early detection and through an efficient clinical treatment. Chemotherapy, radiotherapy and surgery are the most important approach for the treatment of several ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Human Defensins: Synthesis and Structural Properties
Current Pharmaceutical Design Mass Spectrometry Techniques: Principles and Practices for Quantitative Proteomics
Current Protein & Peptide Science NOTCH Signaling as a Novel Cancer Therapeutic Target
Current Cancer Drug Targets Ex Vivo Models of HIV Sexual Transmission and Microbicide Development
Current HIV Research Multiple Biological Activities of Lactic Acid in Cancer: Influences on Tumor Growth,Angiogenesis and Metastasis
Current Pharmaceutical Design Circulating Tumor Cells: A Reliable Biomarker for Prostate Cancer Treatment Assessment?
Current Drug Metabolism Natural Compounds with Proteasome Inhibitory Activity for Cancer Prevention and Treatment
Current Protein & Peptide Science Recent Patents Concerning Modulators of Protein Kinase C
Recent Patents on DNA & Gene Sequences The Molecular Basis of Notch Signaling Regulation: A Complex Simplicity
Current Molecular Medicine Synthesis, Structure Activity Relationship (SAR), and Biological Activities of Benzylideneacetophenones Derivatives
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Effects of Natural Products on Mcl-1 Expression and Function
Current Medicinal Chemistry Study of Cancer Cell Lines with Fourier Transform Infrared (FTIR)/ Vibrational Absorption (VA) Spectroscopy
Current Physical Chemistry Cigarette Smoking, Metabolic Activation and Carcinogenesis
Current Drug Metabolism Antidotal Effects of Curcumin Against Agents-Induced Cardiovascular Toxicity
Cardiovascular & Hematological Disorders-Drug Targets Chemical Constituents of Plants from the Genus Valeriana
Mini-Reviews in Organic Chemistry Peptides in Oral Diseases
Current Pharmaceutical Design Current and Future Applications for Stem Cell Therapies in Spine Surgery
Current Stem Cell Research & Therapy T-Cell Zeta Chain Expression, Phosphorylation and Degradation and their Role in T-Cell Signal Transduction and Immune Response Regulation in Health And Disease
Current Signal Transduction Therapy Molecular and Clinical Analysis of Predictive Biomarkers in Non-Small-Cell Lung Cancer
Current Medicinal Chemistry The Antiproliferative Effect of Soy (<i>Glycine max</i>) Isoflavones Contained in a Nutraceutical on Cancer Cell Lines
Current Nutraceuticals