Generic placeholder image

Current Pharmaceutical Design

Editor-in-Chief

ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

Review Article

Hydrogen Commonly Applicable from Medicine to Agriculture: From Molecular Mechanisms to the Field

Author(s): Longna Li, Wang Lou, Lingshuai Kong and Wenbiao Shen*

Volume 27, Issue 5, 2021

Published on: 07 December, 2020

Page: [747 - 759] Pages: 13

DOI: 10.2174/1381612826666201207220051

Price: $65

Abstract

The emerging field of hydrogen biology has to date mainly been applied in medicine. However, hydrogen biology can also enable positive outcomes in agriculture. Agriculture faces significant challenges resulting from a growing population, climate change, natural disasters, environmental pollution, and food safety issues. In fact, hydrogen agriculture is a practical application of hydrogen biology, which may assist in addressing many of these challenges. It has been demonstrated that hydrogen gas (H2) may enhance plant tolerance towards abiotic and biotic stresses, regulate plant growth and development, increase nutritional values, prolong the shelf life, and decrease the nitrite accumulation during the storage of vegetables, as well as increase the resilience of livestock to pathogens. Our field trials show that H2 may have a promising potential to increase yield and improve the quality of agricultural products. This review aims to elucidate mechanisms for a novel agricultural application of H2 in China. Future development of hydrogen agriculture is proposed as well. Obviously, hydrogen agriculture belongs to a low carbon economy, and has great potential to provide “safe, tasty, healthy, and high-yield” agricultural products so that it may improve the sustainability of agriculture.

Keywords: Hydrogen gas, hydrogen agriculture, hydrogen biology, molecular mechanism, field trials, low-carbon agriculture.

« Previous
[1]
Dresselhaus MS, Thomas IL. Alternative energy technologies. Nature 2001; 414(6861): 332-7.
[http://dx.doi.org/10.1038/35104599] [PMID: 11713539]
[2]
Gaffron H. Reduction of carbon dioxide with molecular hydrogen in green algae. Nature 1939; 143(3614): 204-5.
[http://dx.doi.org/10.1038/143204a0]
[3]
Gest H, Kamen MD. Studies on the metabolism of photosynthetic bacteria IV: photochemical production of molecular hydrogen by growing cultures of photosynthetic bacteria. J Bacteriol 1949; 58(2): 239-45.
[http://dx.doi.org/10.1128/JB.58.2.239-245.1949] [PMID: 16561777]
[4]
Renwick GM, Giumarro C, Siegel SM. Hydrogen metabolism in higher plants. Plant Physiol 1964; 39(3): 303-6.
[http://dx.doi.org/10.1104/pp.39.3.303] [PMID: 16655917]
[5]
Czerkawski JW. Fate of metabolic hydrogen in the rumen. Proc Nutr Soc 1972; 31(2): 141-6.
[http://dx.doi.org/10.1079/PNS19720028] [PMID: 4563287]
[6]
Ohsawa I, Ishikawa M, Takahashi K, et al. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med 2007; 13(6): 688-94.
[http://dx.doi.org/10.1038/nm1577] [PMID: 17486089]
[7]
Shen W, Sun X. Hydrogen biology: it is just beginning. Chin J Biochem Mol Biol 2019; 35(10): 1037-50.
[http://dx.doi.org/10.13865/j.cnki.cjbmb.2019.10.01]
[8]
Li J, Dong Y, Chen H, et al. Protective effects of hydrogen-rich saline in a rat model of permanent focal cerebral ischemia via reducing oxidative stress and inflammatory cytokines. Brain Res 2012; 1486: 103-11.
[http://dx.doi.org/10.1016/j.brainres.2012.09.031] [PMID: 23010312]
[9]
Liu YQ, Liu YF, Ma XM, et al. Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway. J Plast Reconstr Aesthet Surg 2015; 68(7): e147-56.
[http://dx.doi.org/10.1016/j.bjps.2015.03.001] [PMID: 26003800]
[10]
Matei N, Camara R, Zhang JH. Emerging mechanisms and novel applications of hydrogen gas therapy. Med Gas Res 2018; 8(3): 98-102.
[http://dx.doi.org/10.4103/2045-9912.239959] [PMID: 30319764]
[11]
Jin Q, Zhu K, Cui W, et al. Hydrogen-modulated stomatal sensitivity to abscisic acid and drought tolerance via the regulation of Apoplastic pH in Medicago sativa. J Plant Growth Regul 2016; 35: 565-73.
[http://dx.doi.org/10.1007/s00344-015-9561-2]
[12]
Wu Q, Su N, Chen Q, et al. Cadmium-induced hydrogen accumulation is involved in cadmium tolerance in Brassica campestris by reestablishment of reduced glutathione homeostasis. PLoS One 2015; 10(10)
[http://dx.doi.org/10.1371/journal.pone.0139956] [PMID: 26445361]
[13]
Chen Q, Zhao X, Lei D, et al. Hydrogen-rich water pretreatment alters photosynthetic gas exchange, chlorophyll fluorescence, and antioxidant activities in heat-stressed cucumber leaves. J Plant Growth Regul 2017; 83: 69-82.
[http://dx.doi.org/10.1007/s10725-017-0284-1]
[14]
Lu H, Wu B, Wang Y, et al. Effects of hydrogen-rich water treatment on defense responses of postharvest tomato fruit to Botrytis cinerea. Henan Nongye Kexue 2017; 46(2): 64-8.
[http://dx.doi.org/10.15933/j.cnki.1004-3268.2017.02.013]
[15]
Xu S, Zhu S, Jiang Y, et al. Hydrogen-rich water alleviates salt stress in rice during seed germination. Plant Soil 2013; 370: 47-57.
[http://dx.doi.org/10.1007/s11104-013-1614-3]
[16]
Cao Z, Duan X, Yao P, et al. Hydrogen gas is involved in auxin-induced lateral root formation by modulating nitric oxide synthesis. Int J Mol Sci 2017; 18(10): 2084.
[http://dx.doi.org/10.3390/ijms18102084] [PMID: 28972563]
[17]
Zhu Y, Liao W, Wang M, Niu L, Xu Q, Jin X. Nitric oxide is required for hydrogen gas-induced adventitious root formation in cucumber. J Plant Physiol 2016; 195: 50-8.
[http://dx.doi.org/10.1016/j.jplph.2016.02.018] [PMID: 27010347]
[18]
Su N, Wu Q, Liu Y, et al. Hydrogen-rich water reestablishes ROS homeostasis but exerts differential effects on anthocyanin synthesis in two varieties of radish sprouts under UV-A irradiation. J Agric Food Chem 2014; 62(27): 6454-62.
[http://dx.doi.org/10.1021/jf5019593] [PMID: 24955879]
[19]
Guan Q, Ding XW, Jiang R, et al. Effects of hydrogen-rich water on the nutrient composition and antioxidative characteristics of sprouted black barley. Food Chem 2019; 299.
[http://dx.doi.org/10.1016/j.foodchem.2019.125095] [PMID: 31279124]
[20]
Hu H, Li P, Wang Y, Gu R. Hydrogen-rich water delays postharvest ripening and senescence of kiwifruit. Food Chem 2014; 156: 100-9.
[http://dx.doi.org/10.1016/j.foodchem.2014.01.067] [PMID: 24629944]
[21]
Ren P, Jin X, Liao W, et al. Effect of hydrogen-rich water on vase life and quality in cut lily and rose flowers. Hortic Environ Biotechnol 2017; 58(6): 576-84.
[http://dx.doi.org/10.1007/s13580-017-0043-2]
[22]
Dong Z, Wu L, Kettlewell B, et al. Hydrogen fertilization of soils-is this a benefit of legumes in rotation? Plant Cell Environ 2003; 26(11): 1875-9.
[http://dx.doi.org/10.1046/j.1365-3040.2003.01103.x]
[23]
Bailey-Serres J, Parker JE, Ainsworth EA, Oldroyd GED, Schroeder JI. Genetic strategies for improving crop yields. Nature 2019; 575(7781): 109-18.
[http://dx.doi.org/10.1038/s41586-019-1679-0] [PMID: 31695205]
[24]
Edmeades DC. The long-term effects of manures and fertilisers on soil productivity and quality: a review. Nutr Cycl Agroecosyst 2003; 66: 165-80.
[http://dx.doi.org/10.1023/A:1023999816690]
[25]
Aktar MW, Sengupta D, Chowdhury A. Impact of pesticides use in agriculture: their benefits and hazards. Interdiscip Toxicol 2009; 2(1): 1-12.
[http://dx.doi.org/10.2478/v10102-009-0001-7] [PMID: 21217838]
[26]
Li J, Yue X, Cheng Y, et al. Research progress on heavy metal pollution in soil and the effect on heavy metal residues in vegetables. Food Safe Qual Detec Technol 2019; 10(16): 5299-305.
[http://dx.doi.org/10.2478/v10102-009-0001-7]
[27]
Vignais PM, Billoud B, Meyer J. Classification and phylogeny of hydrogenases. FEMS Microbiol Rev 2001; 25(4): 455-501.
[http://dx.doi.org/10.1016/S0168-6445(01)00063-8] [PMID: 11524134]
[28]
Lubitz W, Ogata H, Rüdiger O, Reijerse E. Hydrogenases. Chem Rev 2014; 114(8): 4081-148.
[http://dx.doi.org/10.1021/cr4005814] [PMID: 24655035]
[29]
Schubert KR, Evans HJ. Hydrogen evolution: A major factor affecting the efficiency of nitrogen fixation in nodulated symbionts. Proc Natl Acad Sci USA 1976; 73(4): 1207-11.
[http://dx.doi.org/10.1073/pnas.73.4.1207] [PMID: 16592307]
[30]
Liu F, Cai B, Sun S, et al. Effect of hydrogen-rich water soaked cucumber seeds on cold tolerance and its physiological mechanism in cucumber seedlings. Zhongguo Nong Ye Ke Xue 2017; 50(5): 881-9.
[http://dx.doi.org/10.3864/j.issn.0578-1752.2017.05.011]
[31]
Chen Y, Wang M, Hu L, Liao W, Dawuda MM, Li C. Carbon monoxide is involved in hydrogen gas-induced adventitious root development in cucumber under simulated drought stress. Front Plant Sci 2017; 8: 128.
[http://dx.doi.org/10.3389/fpls.2017.00128] [PMID: 28223992]
[32]
Xie Y, Mao Y, Lai D, Zhang W, Shen W. H(2) enhances arabidopsis salt tolerance by manipulating ZAT10/12-mediated antioxidant defence and controlling sodium exclusion. PLoS One 2012; 7(11)
[http://dx.doi.org/10.1371/journal.pone.0049800] [PMID: 23185443]
[33]
Cui W, Gao C, Fang P, Lin G, Shen W. Alleviation of cadmium toxicity in Medicago sativa by hydrogen-rich water. J Hazard Mater 2013; 260(15): 715-24.
[http://dx.doi.org/10.1016/j.jhazmat.2013.06.032] [PMID: 23846121]
[34]
Cui W, Fang P, Zhu K, et al. Hydrogen-rich water confers plant tolerance to mercury toxicity in alfalfa seedlings. Ecotoxicol Environ Saf 2014; 105: 103-11.
[http://dx.doi.org/10.1016/j.ecoenv.2014.04.009] [PMID: 24793520]
[35]
Jin Q, Zhu K, Cui W, Xie Y, Han B, Shen W. Hydrogen gas acts as a novel bioactive molecule in enhancing plant tolerance to paraquat-induced oxidative stress via the modulation of heme oxygenase-1 signalling system. Plant Cell Environ 2013; 36(5): 956-69.
[http://dx.doi.org/10.1111/pce.12029] [PMID: 23094798]
[36]
Xie Y, Mao Y, Zhang W, Lai D, Wang Q, Shen W. Reactive oxygen species-dependent nitric oxide production contributes to hydrogen-promoted stomatal closure in Arabidopsis. Plant Physiol 2014; 165(2): 759-73.
[http://dx.doi.org/10.1104/pp.114.237925] [PMID: 24733882]
[37]
Zeng J, Zhang M, Sun X. Molecular hydrogen is involved in phytohormone signaling and stress responses in plants. PLoS One 2013; 8(8)e71038
[http://dx.doi.org/10.1371/journal.pone.0071038] [PMID: 23951075]
[38]
Su J, Nie Y, Zhao G, et al. Endogenous hydrogen gas delays petal senescence and extengs the vase life of lisianthus cut flowers. Postharvest Biol Technol 2019; 147: 148-55.
[http://dx.doi.org/10.1016/j.postharvbio.2018.09.018]
[39]
Appel J, Schulz R. Hydrogen metabolism in organisms with oxygenic photosynthesis: hydrogenases as important regulatory devices for a proper redox poising? J Photochem Photobiol Ser B 1998; 47: 1-11.
[http://dx.doi.org/10.1016/S1011-1344(98)00179-1]
[40]
Tamagnini P, Axelsson R, Lindberg P, Oxelfelt F, Wünschiers R, Lindblad P. Hydrogenases and hydrogen metabolism of cyanobacteria. Microbiol Mol Biol Rev 2002; 66(1): 1-20.
[http://dx.doi.org/10.1128/MMBR.66.1.1-20.2002] [PMID: 11875125]
[41]
Irvine P, Smith M, Dong Z. Hydrogen fertilizer: bacteria or fungi? Acta Hortic 2004; (631): 239-42.
[http://dx.doi.org/10.17660/ActaHortic.2004.631.30]
[42]
Liu H, Wang W, Cao G, et al. Effect of hydrogen on microbial population and enzyme activity in Robinia pseudoacacia rhizosphere soil. Chin J Appl Environ Biol 2010; 16(4): 515-8.
[http://dx.doi.org/10.3724/SP.J.1145.2010.00515]
[43]
Golding AL, Dong Z. Hydrogen production by nitrogenase as a potential crop rotation benefit. Environ Chem Lett 2010; 8(2): 101-21.
[http://dx.doi.org/10.1007/s10311-010-0278-y]
[44]
McLearn N, Dong Z. Microbial nature of the hydrogen-oxidizing agent in hydrogen treated soil. Biol Fertil Soils 2002; 35: 465-9.
[http://dx.doi.org/10.1007/s00374-002-0495-z]
[45]
Stein S, Selesi D, Schilling R, et al. Microbial activity and bacterial composition of H2-treated soils with net CO2 fixation. Soil Biol Biochem 2005; 37(10): 1938-45.
[http://dx.doi.org/10.1016/j.soilbio.2005.02.035]
[46]
Dong Z, Layzell DBH. 2 oxidation, O2 uptake and CO2 fixation in hydrogen treated soils. Plant Soil 2001; 229(1): 1-12.
[http://dx.doi.org/10.1023/A:1004810017490]
[47]
Ren A, Liu R, Miao ZG, et al. Hydrogen-rich water regulates effects of ROS balance on morphology, growth and secondary metabolism via glutathione peroxidase in Ganoderma lucidum. Environ Microbiol 2017; 19(2): 566-83.
[http://dx.doi.org/10.1111/1462-2920.13498] [PMID: 27554678]
[48]
Zhang J, Hao H, Chen M, Wang H, Feng Z, Chen H. Hydrogen-rich water alleviates the toxicities of different stresses to mycelial growth in Hypsizygus marmoreus. AMB Express 2017; 7(1): 107.
[http://dx.doi.org/10.1186/s13568-017-0406-1] [PMID: 28565883]
[49]
Chen H, Zhang J, Hao H, et al. Hydrogen-rich water increases postharvest quality by enhancing antioxidant capacity in Hypsizygus marmoreus. AMB Express 2017; 7(1): 221.
[http://dx.doi.org/10.1186/s13568-017-0496-9] [PMID: 29264772]
[50]
Xu D, Cao H, Fang W, et al. Linking hydrogen-enhanced rice aluminum tolerance with the reestablishment of GA/ABA balance and miRNA-modulated gene expression: A case study on germination. Ecotoxicol Environ Saf 2017; 145: 303-12.
[http://dx.doi.org/10.1016/j.ecoenv.2017.07.055] [PMID: 28756251]
[51]
Zhu Y, Liao W. The metabolic constituent and rooting-related enzymes responses of marigold explants to hydrogen gas during adventitious root development. Theor Exp Plant Physiol 2017; 29(2): 77-85.
[http://dx.doi.org/10.1007/s40626-017-0085-y]
[52]
Wang Y, Wei C. Effect of hydrogen-rich water concentration on rooting of nepenthes. Mod Agri Sci Technol 2016; 14: 136-7.
[http://dx.doi.org/10.3969/j.issn.1007-5739.2016.14.079]
[53]
Zhu Y, Liao W, Niu L, Wang M, Ma Z. Nitric oxide is involved in hydrogen gas-induced cell cycle activation during adventitious root formation in cucumber. BMC Plant Biol 2016; 16(1): 146.
[http://dx.doi.org/10.1186/s12870-016-0834-0] [PMID: 27352869]
[54]
Zhang X, Wei J, Tian J, et al. Enhanced anthocyanin accumulation of immature radish microgreens by hydrogen-rich water under short wavelength light. Sci Hortic (Amsterdam) 2019; 247: 75-85.
[http://dx.doi.org/10.1016/j.scienta.2018.11.060]
[55]
Xie Y, Zhang W, Duan X, et al. Hydrogen-rich water-alleviated ultraviolet-B-triggered oxidative damage is partially associated with the manipulation of the metabolism of (iso)flavonoids and antioxidant defence in Medicago sativa. Funct Plant Biol 2015; 42(12): 1141-57.
[http://dx.doi.org/10.1071/FP15204] [PMID: 32480752]
[56]
Zhang X, Su N, Jia L, et al. Transcriptome analysis of radish sprouts hypocotyls reveals the regulatory role of hydrogen-rich water in anthocyanin biosynthesis under UV-A. BMC Plant Biol 2018; 18(1): 227.
[http://dx.doi.org/10.1186/s12870-018-1449-4] [PMID: 30305047]
[57]
Su J, Zhang Y, Nie Y, et al. Hydrogen-induced osmotic tolerance is associated with nitric oxide-mediated proline accumulation and reestablishment of redox balance in alfalfa seedlings. Environ Exp Bot 2018; 147: 249-60.
[http://dx.doi.org/10.1016/j.envexpbot.2017.12.022]
[58]
Zhang Y, Cheng P, Wang Y, et al. Genetic elucidation of hydrogen signaling in plant osmotic tolerance and stomatal closure via hydrogen sulfide. Free Radic Biol Med 2020; 161: 1-14.
[http://dx.doi.org/10.1016/j.freeradbiomed.2020.09.021] [PMID: 32987125]
[59]
Su J, Yang X, Shao Y, Chen Z, Shen W. Molecular hydrogen-induced salinity tolerance requires melatonin signalling in Arabidopsis thaliana. Plant Cell Environ 2020.
[http://dx.doi.org/10.1111/pce.13926] [PMID: 33103784]
[60]
Dai C, Cui W, Pan J, Xie Y, Wang J, Shen W. Proteomic analysis provides insights into the molecular bases of hydrogen gas-induced cadmium resistance in Medicago sativa. J Proteomics 2017; 152: 109-20.
[http://dx.doi.org/10.1016/j.jprot.2016.10.013] [PMID: 27989938]
[61]
Wu Q, Su N, Cai J, Shen Z, Cui J. Hydrogen-rich water enhances cadmium tolerance in Chinese cabbage by reducing cadmium uptake and increasing antioxidant capacities. J Plant Physiol 2015; 175: 174-82.
[http://dx.doi.org/10.1016/j.jplph.2014.09.017] [PMID: 25543863]
[62]
Chen M, Cui W, Zhu K, Xie Y, Zhang C, Shen W. Hydrogen-rich water alleviates aluminum-induced inhibition of root elongation in alfalfa via decreasing nitric oxide production. J Hazard Mater 2014; 267: 40-7.
[http://dx.doi.org/10.1016/j.jhazmat.2013.12.029] [PMID: 24413050]
[63]
Mittler R, Vanderauwera S, Gollery M, Van Breusegem F. Reactive oxygen gene network of plants. Trends Plant Sci 2004; 9(10): 490-8.
[http://dx.doi.org/10.1016/j.tplants.2004.08.009] [PMID: 15465684]
[64]
Xu S, Jiang Y, Cui W, et al. Hydrogen enhances adaptation of rice seedlings to cold stress via the reestablishment of redox homeostasis mediated by miRNA expression. Plant Soil 2017; 414(1-2): 53-67.
[http://dx.doi.org/10.1007/s11104-016-3106-8]
[65]
Miwa K, Takano J, Omori H, Seki M, Shinozaki K, Fujiwara T. Plants tolerant of high boron levels. Science 2007; 318(5855): 1417.
[http://dx.doi.org/10.1126/science.1146634] [PMID: 18048682]
[66]
Wang Y, Duan X, Xu S, Wang R, Ouyang Z, Shen W. Linking hydrogen-mediated boron toxicity tolerance with improvement of root elongation, water status and reactive oxygen species balance: a case study for rice. Ann Bot 2016; 118(7): 1279-91.
[http://dx.doi.org/10.1093/aob/mcw181] [PMID: 27616208]
[67]
Liu F, Li J, Liu Y. Molecular hydrogen can take part in phytohormone signal pathways in wild rice. Biol Plant 2016; 60(2): 311-9.
[http://dx.doi.org/10.1007/s10535-016-0591-9]
[68]
Hu H, Zhao S, Li P, et al. Hydrogen gas prolongs the shelf life of kiwifruit by decreasing ethylene biosynthesis. Postharvest Biol Technol 2018; 135: 123-30.
[http://dx.doi.org/10.1016/j.postharvbio.2017.09.008]
[69]
Ren P, Li X, Xu X, et al. Effects of hydrogen gas on vase life and quality of cut lily. Gansu Nongye Daxue Xuebao 2017; 52(1): 103-8.
[http://dx.doi.org/10.13432/j.cnki.jgsau.2017.01.018]
[70]
Wang C, Fang H, Gong T, et al. Hydrogen gas alleviates postharvest senescence of cut rose ‘Movie star’ by antagonizing ethylene. Plant Mol Biol 2020; 102(3): 271-85.
[http://dx.doi.org/10.1007/s11103-019-00946-3] [PMID: 31838617]
[71]
Huo J, Huang D, Zhang J, et al. Comparative proteomic analysis during the involvement of nitric oxide in hydrogen gas-improved postharvest freshness in cut lilies. Int J Mol Sci 2018; 19(12): 3955.
[http://dx.doi.org/10.3390/ijms19123955] [PMID: 30544843]
[72]
Hsu J, Arcot J, Lee NA. Nitrate and nitrite quantification from cured meat and vegetables and their estimated dietary intake in Australians. Food Chem 2009; 115: 334-9.
[http://dx.doi.org/10.1016/j.foodchem.2008.11.081]
[73]
Prasad S, Chetty AA. Nitrate-N determination in leafy vegetables: study of the effects of cooking and freezing. Food Chem 2008; 106(2): 772-80.
[http://dx.doi.org/10.1016/j.foodchem.2007.06.005]
[74]
Zhang Y, Zhao G, Cheng P, et al. Nitrite accumulation during storage of tomato fruit as prevented by hydrogen gas. Int J Food Prop 2019; 22(1): 1425-38.
[http://dx.doi.org/10.1080/10942912.2019.1651737]
[75]
Zheng W, Ji X, Zhang Q, Yao W. Intestinal microbiota ecological response to oral administrations of hydrogen-rich water and lactulose in female piglets fed a Fusarium toxin-contaminated diet. Toxins (Basel) 2018; 10(6): 246.
[http://dx.doi.org/10.3390/toxins10060246] [PMID: 29914163]
[76]
Zheng W, Ji X, Zhang Q, Du W, Wei Q, Yao W. Hydrogen-rich water and lactulose protect against growth suppression and oxidative stress in female piglets fed Fusarium toxins contaminated diets. Toxins (Basel) 2018; 10(6): 228.
[http://dx.doi.org/10.3390/toxins10060228] [PMID: 29867031]
[77]
Ji X, Zhang Q, Zheng W, Yao W. Morphological and molecular response of small intestine to lactulose and hydrogen-rich water in female piglets fed Fusarium mycotoxins contaminated diet. J Anim Sci Biotechnol 2019; 10: 9.
[http://dx.doi.org/10.1186/s40104-019-0320-2] [PMID: 30805184]
[78]
Wang M, Wang R, Zhang X, et al. Molecular hydrogen generated by elemental magnesium supplementation alters rumen fermentation and microbiota in goats. Br J Nutr 2017; 118(6): 401-10.
[http://dx.doi.org/10.1017/S0007114517002161] [PMID: 28927478]
[79]
Kawamura T, Wakabayashi N, Shigemura N, et al. Hydrogen gas reduces hyperoxic lung injury via the Nrf2 pathway in vivo. Am J Physiol Lung Cell Mol Physiol 2013; 304(10): L646-56.
[http://dx.doi.org/10.1152/ajplung.00164.2012] [PMID: 23475767]
[80]
Guo SX, Fang Q, You CG, et al. Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation. J Transl Med 2015; 13: 183.
[http://dx.doi.org/10.1186/s12967-015-0548-3] [PMID: 26047940]
[81]
Xin HG, Zhang BB, Wu ZQ, et al. Consumption of hydrogen-rich water alleviates renal injury in spontaneous hypertensive rats. Mol Cell Biochem 2014; 392(1-2): 117-24.
[http://dx.doi.org/10.1007/s11010-014-2024-4] [PMID: 24652103]
[82]
Song G, Tian H, Liu J, Zhang H, Sun X, Qin S. H2 inhibits TNF-α-induced lectin-like oxidized LDL receptor-1 expression by inhibiting nuclear factor κB activation in endothelial cells. Biotechnol Lett 2011; 33(9): 1715-22.
[http://dx.doi.org/10.1007/s10529-011-0630-8] [PMID: 21544615]
[83]
Runtuwene J, Amitani H, Amitani M, Asakawa A, Cheng KC, Inui A. Hydrogen-water enhances 5-fluorouracil-induced inhibition of colon cancer. PeerJ 2015; 3.
[http://dx.doi.org/10.7717/peerj.859] [PMID: 25870767]
[84]
Ono H, Nishijima Y, Ohta S, et al. Hydrogen gas inhalation treatment in acute cerebral infarction: a randomized controlled clinical study on safety and neuroprotection. J Stroke Cerebrovasc Dis 2017; 26(11): 2587-94.
[http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2017.06.012] [PMID: 28669654]
[85]
Katsumata Y, Sano F, Abe T, et al. The effects of hydrogen gas inhalation on adverse left ventricular remodeling after percutaneous coronary intervention for ST-elevated myocardial infarction-first pilot study in humans. Circ J 2017; 81(7): 940-7.
[http://dx.doi.org/10.1253/circj.CJ-17-0105] [PMID: 28321000]
[86]
Ohta S. Will the hydrogen therapy be approved shortly? Ann Transl Med 2020; 8(6): 264.
[http://dx.doi.org/10.21037/atm.2020.03.70] [PMID: 32355708]
[87]
Kajiyama S, Hasegawa G, Asano M, et al. Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance. Nutr Res 2008; 28(3): 137-43.
[http://dx.doi.org/10.1016/j.nutres.2008.01.008] [PMID: 19083400]
[88]
Mizuno H, Ekuni D, Maruyama T, et al. The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trial. PLoS One 2017; 12(11)e0188171
[http://dx.doi.org/10.1371/journal.pone.0188171] [PMID: 29145468]
[89]
Nakao A, Toyoda Y, Sharma P, Evans M, Guthrie N. Effectiveness of hydrogen rich water on antioxidant status of subjects with potential metabolic syndrome-an open label pilot study. J Clin Biochem Nutr 2010; 46(2): 140-9.
[http://dx.doi.org/10.3164/jcbn.09-100] [PMID: 20216947]
[90]
Song G, Li M, Sang H, et al. Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome. J Lipid Res 2013; 54(7): 1884-93.
[http://dx.doi.org/10.1194/jlr.M036640] [PMID: 23610159]
[91]
Ichihara M, Sobue S, Ito M, Ito M, Hirayama M, Ohno K. Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive review of 321 original articles. Med Gas Res 2015; 5(1): 12.
[http://dx.doi.org/10.1186/s13618-015-0035-1] [PMID: 26483953]
[92]
Ishibashi T, Sato B, Rikitake M, et al. Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis: an open-label pilot study. Med Gas Res 2012; 2(1): 27.
[http://dx.doi.org/10.1186/2045-9912-2-27] [PMID: 23031079]
[93]
Nakayama M, Itami N, Suzuki H, et al. Possible clinical effects of molecular hydrogen (H2) delivery during hemodialysis in chronic dialysis patients: Interim analysis in a 12 month observation. PLoS One 2017; 12(9)e0184535
[http://dx.doi.org/10.1371/journal.pone.0184535] [PMID: 28902900]
[94]
Nakayama M, Itami N, Suzuki H, et al. Novel haemodialysis (HD) treatment employing molecular hydrogen (H2)-enriched dialysis solution improves prognosis of chronic dialysis patients: A prospective observational study. Sci Rep 2018; 8(1): 254.
[http://dx.doi.org/10.1038/s41598-017-18537-x] [PMID: 29321509]
[95]
Sakai T, Sato B, Hara K, et al. Consumption of water containing over 3.5 mg of dissolved hydrogen could improve vascular endothelial function. Vasc Health Risk Manag 2014; 10: 591-7.
[http://dx.doi.org/10.2147/VHRM.S68844] [PMID: 25378931]
[96]
Ishibashi T, Kawamoto K, Matsuno K, Ishihara G, Baba T, Komori N. Peripheral endothelial function can be improved by daily consumption of water containing over 7 ppm of dissolved hydrogen: A randomized controlled trial. PLoS One 2020; 15(5)e0233484
[http://dx.doi.org/10.1371/journal.pone.0233484] [PMID: 32470022]
[97]
Shen W, Xie Y, Cao Z, et al. The preparation method and application of hydrogen-rich liquid plant growth regulator. China patent CN 102657221B, 2014.
[98]
Alwazeer D, Tan K, Örs B. Reducing atmosphere packaging as a novel alternative technique for extending shelf life of fresh cheese. J Food Sci Technol 2020; 57(8): 3013-23.
[http://dx.doi.org/10.1007/s13197-020-04334-4] [PMID: 32624604]
[99]
Alwazeer D, Delbeau C, Divies C, Cachon R. Use of redox potential modification by gas improves microbial quality, color retention, and ascorbic acid stability of pasteurized orange juice. Int J Food Microbiol 2003; 89(1): 21-9.
[http://dx.doi.org/10.1016/S0168-1605(03)00125-9] [PMID: 14580970]
[100]
Alwazeer D, Örs B. Reducing atmosphere drying as a novel drying technique for preserving the sensorial and nutritional notes of foods. J Food Sci Technol 2019; 56(8): 3790-800.
[http://dx.doi.org/10.1007/s13197-019-03850-2] [PMID: 31413405]
[101]
Suzuki Y, Sano M, Hayashida K, Ohsawa I, Ohta S, Fukuda K. Are the effects of α-glucosidase inhibitors on cardiovascular events related to elevated levels of hydrogen gas in the gastrointestinal tract? FEBS Lett 2009; 583(13): 2157-9.
[http://dx.doi.org/10.1016/j.febslet.2009.05.052] [PMID: 19505462]
[102]
Safonov V, Khitrin A. Hydrogen nanobubbles in a water solution of dietary supplement. Colloids Surf A Physicochem Eng Asp 2013; 436: 333-6.
[http://dx.doi.org/10.1016/j.colsurfa.2013.06.043]
[103]
Cai M, Du HM. Effects of hydrogen-rich water pretreatment on vase life of carnation (Dianthus caryophyllus) cut flowers. Shanghai Jiaotong Daxue Xuebao Nongye Kexueban 2015; 33(6): 41-5.
[http://dx.doi.org/10.3969/J.ISSN.1671-9964.2015.06.0007]
[104]
Song Y, Cong F, Li C, et al. Effects of hydrogen-rich water pretreatment on vase life and antioxidant system in cut freesia. Shanghai Jiaotong Daxue Xuebao Nongye Kexueban 2018; 36(1): 1-6, 13.
[http://dx.doi.org/10.3969/J.ISSN.1671-9964.2018.01.001]
[105]
Shen W, Zhang Y, Su J, et al. The preparation and application of hydrogen-based degradation agent reducing the accumulation of nitrite in fruits and vegetables after harvest. China patent CN106901142B, 2020.
[106]
Fontanari P, Badier M, Guillot C, et al. Changes in maximal performance of inspiratory and skeletal muscles during and after the 7.1-MPa Hydra 10 record human dive. Eur J Appl Physiol 2000; 81(4): 325-8.
[http://dx.doi.org/10.1007/s004210050050] [PMID: 10664092]
[107]
Hayashida K, Sano M, Ohsawa I, et al. Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia-reperfusion injury. Biochem Biophys Res Commun 2008; 373(1): 30-5.
[http://dx.doi.org/10.1016/j.bbrc.2008.05.165] [PMID: 18541148]
[108]
Kamimura N, Nishimaki K, Ohsawa I, Ohta S. Molecular hydrogen improves obesity and diabetes by inducing hepatic FGF21 and stimulating energy metabolism in db/db mice. Obesity (Silver Spring) 2011; 19(7): 1396-403.
[http://dx.doi.org/10.1038/oby.2011.6] [PMID: 21293445]

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy