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Current Nutraceuticals

Editor-in-Chief

ISSN (Print): 2665-9786
ISSN (Online): 2665-9794

Research Article

Anticancer Potential of Lepidium Sativum Seeds Aqueous Extract on the Azoxymethane/ Dextran Sulfate Sodium-Induced Colon Cancer In vivo

Author(s): Nahed Ahmed Hussien* and Ghazwaa Awad Alsulami

Volume 2 , Issue 1 , 2021

Published on: 28 September, 2020

Page: [78 - 88] Pages: 11

DOI: 10.2174/2665978601999200928212236

Abstract

Background: Colon cancer is responsible for increasing the death rate worldwide. Commonly used anticancer drugs have various side effects and their clinical usage must be restricted due to their toxicity.

Objective: The present research aimed to evaluate the anticancer potential of Lepidium sativum L. (LS) seeds aqueous extract against azoxymethane/dextran sodium sulfate (AOM/DSS) inducedcolon cancer in male albino mice.

Methods: Low (200 mg/kg) and high (400 mg/kg) doses of LS seeds extract were used to treat induced colon cancer in different stages.

Results: The present results report that LS treatment for mice with colon cancer especially in high dose, decreases colon polyps/tumor incidence and size, tissues disorder, expression of P53 and increases apoptosis in colon tissue. Moreover, LS decreases micronucleus induction in polychromatic (PCE), increases PCE/normochromatic erythrocytes ratio and decreases the percentage of sperm abnormalities.

Conclusion: The present study reports anticancer potential of LS for induced colorectal cancer mice by ameliorating the inflammatory steps of colon.

Keywords: Lepidium sativum, colitis, colorectal cancer, azoxymethane, dextran sodium sulfate, male albino mice.

Graphical Abstract
[1]
GLOBOCAN 2012: Estimated Cancer Incidence, Mortality and Prevalence Worldwide in 2012. Available at: , http://globocan. iarc.fr/default.aspx(Accessed on May 1, 2020).
[2]
Forman, D.; Bray, F.; Brewster, D.H.; Gombe Mbalawa, C.; Kohler, B.; Piñeros, M.; Steliarova-Foucher, E.; Swaminathan, R.; Ferlay, J. Cancer incidence in five continents.IARC, 2014., 10, 126-140.,
[3]
Saltz, L.B.; Douillard, J.Y.; Pirotta, N.; Alakl, M.; Gruia, G.; Awad, L.; Elfring, G.L.; Locker, P.K.; Miller, L.L. Irinotecan plus fluorouracil/leucovorin for metastatic colorectal cancer: A new survival standard. Oncologist, 2001, 6(1), 81-91.
[http://dx.doi.org/10.1634/theoncologist.6-1-81] [PMID: 11161231]
[4]
Niccolai, E.; Amedei, A. Vaccine immunotherapy strategies in colorectal cancer treatment. Single Cell Biol., 2012, 1, 102.
[http://dx.doi.org/10.4172/2168-9431.1000102]
[5]
Timofeiov, S.; Marinca, M.; Bar, C.; Breabăn, M.E.; Drug, V.; Scripcariu, V. Conversion rate to resectability in colorectal cancer liver metastases: Need for criteria adapted to current therapy. J. Surg. (Northborough), 2015, 11, 323-336.
[6]
Turner, J.; Page, M.; Clark, C.; Khanduja, K. Sigmoid perforation during CT colonography in a patient with an inguinal hernia and concomitant finding of a right-sided colon cancer. J. Gastrointest. Dig. Syst., 2016, 6, 378.
[7]
Lenz, H.J.; Van Cutsem, E.; Khambata-Ford, S.; Mayer, R.J.; Gold, P.; Stella, P.; Mirtsching, B.; Cohn, A.L.; Pippas, A.W.; Azarnia, N.; Tsuchihashi, Z.; Mauro, D.J.; Rowinsky, E.K. Multicenter phase II and translational study of cetuximab in metastatic colorectal carcinoma refractory to irinotecan, oxaliplatin, and fluoropyrimidines. J. Clin. Oncol., 2006, 24(30), 4914-4921.
[http://dx.doi.org/10.1200/JCO.2006.06.7595] [PMID: 17050875]
[8]
Giantonio, B.J.; Catalano, P.J.; Meropol, N.J.; O’Dwyer, P.J.; Mitchell, E.P.; Alberts, S.R.; Schwartz, M.A.; Benson, A.B. III eastern cooperative oncology group study E3200. Bevacizumab in combination with oxaliplatin, fluorouracil, and leucovorin (FOLFOX4) for previously treated metastatic colorectal cancer: results from the Eastern cooperative oncology group study E3200. J. Clin. Oncol., 2007, 25(12), 1539-1544.
[http://dx.doi.org/10.1200/JCO.2006.09.6305] [PMID: 17442997]
[9]
Hochster, H.S.; Hart, L.L.; Ramanathan, R.K.; Childs, B.H.; Hainsworth, J.D.; Cohn, A.L.; Wong, L.; Fehrenbacher, L.; Abubakr, Y.; Saif, M.W.; Schwartzberg, L.; Hedrick, E. Safety and efficacy of oxaliplatin and fluoropyrimidine regimens with or without bevacizumab as first-line treatment of metastatic colorectal cancer: results of the TREE Study. J. Clin. Oncol., 2008, 26(21), 3523-3529.
[http://dx.doi.org/10.1200/JCO.2007.15.4138] [PMID: 18640933]
[10]
Cheng, Y.H.; Wong, E.W.; Cheng, C.Y. Cancer/testis (CT) antigens, carcinogenesis and spermatogenesis. Spermatogenesis, 2011, 1(3), 209-220.
[http://dx.doi.org/10.4161/spmg.1.3.17990] [PMID: 22319669]
[11]
Fischer, M.; Yen, W.C.; Kapoun, A.M.; Wang, M.; O’Young, G.; Lewicki, J.; Gurney, A.; Hoey, T. Anti-DLL4 inhibits growth and reduces tumor-initiating cell frequency in colorectal tumors with oncogenic KRAS mutations. Cancer Res., 2011, 71(5), 1520-1525.
[http://dx.doi.org/10.1158/0008-5472.CAN-10-2817] [PMID: 21193546]
[12]
Erdogan, H.; Kemal, N. Protection from side effects of anticancer drugs.OMICS GROUP conferences, 2014, 6, 1948-5956.,
[13]
Sharma, S.; Agrawal, N. Nourishing and healing process of garden cress (Lepidium sativum Linn.)- A review. Indian J. Nat. Prod. Resour., 2011, 2, 292-297.
[14]
Mahassni, S.H.; Al-Reemi, R.M. Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (Lepidium sativum) seeds. Saudi J. Biol. Sci., 2013, 20(2), 131-139.
[http://dx.doi.org/10.1016/j.sjbs.2012.12.002] [PMID: 23961228]
[15]
Mahassni, S.H.; Al-Reemi, R.M. Cytotoxic effect of an aqueous extract of Lepidium sativum L. seeds on human breast cancer cells. Indian J. Tradit. Knowl., 2013, 12, 605-614.
[16]
Doke, S.; Guha, M. Garden cress (Lepidium sativum L.) Seed - An important medicinal source: A review. J. Nat. Prod. Plant Resour., 2014, 4, 69-80.
[17]
Juma, Ab. The effects of Lepidium sativum seeds on fracture-induced healing in rabbits. MedGenMed, 2007, 9(2), 23.
[PMID: 17955079]
[18]
Yadav, Y.C.; Jain, A.; Srivastava, D.N.; Jain, A. Fracture healing activity of ethanolic extract of Lepidium sativum L. seeds in internally fixed rats’ femoral osteotomy model. Int. J. Pharm. Pharm. Sci., 2011, 3, 193-197.
[19]
Paranjape, A.N.; Mehta, A.A. A Study on clinical efficacy of Lepidium sativum seeds in treatment of bronchial asthma. IJPT, 2006, 5, 55-59.
[20]
Al-Yahya, M.A.; Mossa, J.S.; Ageel, A.M.; Rafatullah, S. Pharmacological and safety evaluation studies on Lepidium sativum L., Seeds. Phytomedicine, 1994, 1(2), 155-159.
[http://dx.doi.org/10.1016/S0944-7113(11)80035-8] [PMID: 23195890]
[21]
Datta, P.K.; Diwakar, B.K.; Viswanatha, S.; Murthy, K.N.; Naidu, K.A. Safety evaluation studies on garden cress (Lepidium sativum L.) seeds in Wistar rats. Int. J. Appl. Res. Nat. Prod., 2011, 4, 37-42.
[22]
Raghavendra, R.H.; Akhilender, N.K. Eugenol and n-3 rich garden cress seed oil as modulators of platelet aggregation and eicosanoids in Wistar albino rats. Open Nutraceuticals J., 2011, 4, 144-150.
[http://dx.doi.org/10.2174/1876396001104010144]
[23]
Moser, B.R.; Shah, S.N.; Winkler-Moser, J.K.; Vaughn, S.F.; Evangelista, R.L. Composition and physical properties of cress (Lepidium sativum L.) and field pennycress (Thlaspi arvense L.) oils. Ind. Crops Prod., 2009, 30, 199-205.
[http://dx.doi.org/10.1016/j.indcrop.2009.03.007]
[24]
Gokavi, S.S.; Malleshi, N.G.; Guo, M. Chemical composition of garden cress (Lepidium sativum) seeds and its fractions and use of bran as a functional ingredient. Plant Foods Hum. Nutr., 2004, 59(3), 105-111.
[http://dx.doi.org/10.1007/s11130-004-4308-4] [PMID: 15678716]
[25]
Williams, D.J.; Critchley, C.; Pun, S.; Chaliha, M.; O’Hare, T.J. Differing mechanisms of simple nitrile formation on glucosinolate degradation in Lepidium sativum and Nasturtium officinale seeds. Phytochemistry, 2009, 70(11-12), 1401-1409.
[http://dx.doi.org/10.1016/j.phytochem.2009.07.035] [PMID: 19747700]
[26]
Mahassni, S.H.; Khudauardi, E.R. A Pilot Study: The Effects of an Aqueous Extract of Lepidium sativum Seeds on Levels of Immune Cells and Body and Organs Weights in Mice. J. Ayu. Her. Integr. Med., 2017, 3, 27-32.
[27]
Raish, M.; Ahmad, A.; Alkharfy, K.M.; Ahamad, S.R.; Mohsin, K.; Al-Jenoobi, F.I.; Al-Mohizea, A.M.; Ansari, M.A. Hepatoprotective activity of Lepidium sativum seeds against D-galactosamine/lipopolysaccharide induced hepatotoxicity in animal model. BMC Complement. Altern. Med., 2016, 16(1), 501.
[http://dx.doi.org/10.1186/s12906-016-1483-4] [PMID: 27912738]
[28]
Thaker, A.I.; Shaker, A.; Rao, M.S.; Ciorba, M.A. Modeling colitis-associated cancer with azoxymethane (AOM) and dextran sulfate sodium (DSS). J. Vis. Exp., 2012, 11(67), 4100.
[http://dx.doi.org/10.3791/4100] [PMID: 22990604]
[29]
Alsulami, G.A.; Hussien, N.A. Subchronic study of sperm morphology, genotoxic and mutagenic effect of Lepidium sativum seeds aqueous extract in vivo. Recent Res. Gen. Genom., 2020, 2, 1-14.
[http://dx.doi.org/10.21608/rrgg.2020.21025.1002]
[30]
Ogino, S.; Brahmandam, M.; Cantor, M.; Namgyal, C.; Kawasaki, T.; Kirkner, G.; Meyerhardt, J.A.; Loda, M.; Fuchs, C.S. Distinct molecular features of colorectal carcinoma with signet ring cell component and colorectal carcinoma with mucinous component. Mod. Pathol., 2006, 19(1), 59-68.
[http://dx.doi.org/10.1038/modpathol.3800482] [PMID: 16118624]
[31]
Sambrook, J.; Fritsch, E.F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed; CSH Cold Spring Harbor Press: NY, 1989.
[32]
Dai, J.G.; Min, J.X.; Xiao, Y.B.; Lei, X.; Shen, W.H.; Wei, H. The absence of mitochondrial DNA diversity among common laboratory inbred mouse strains. J. Exp. Biol., 2005, 208(Pt 23), 4445-4450.
[http://dx.doi.org/10.1242/jeb.01920] [PMID: 16339865]
[33]
Schmid, W. The micronucleus test. Mutat. Res., 1975, 31(1), 9-15.
[http://dx.doi.org/10.1016/0165-1161(75)90058-8] [PMID: 48190]
[34]
Watanabe, T.; Endo, A. Effects of selenium deficiency on sperm morphology and spermatocyte chromosomes in mice. Mutat. Res., 1991, 262(2), 93-99.
[http://dx.doi.org/10.1016/0165-7992(91)90113-I] [PMID: 2000100]
[35]
Tanaka, T.; Kohno, H.; Suzuki, R.; Yamada, Y.; Sugie, S.; Mori, H. A novel inflammation-related mouse colon carcinogenesis model induced by azoxymethane and dextran sodium sulfate. Cancer Sci., 2003, 94(11), 965-973.
[http://dx.doi.org/10.1111/j.1349-7006.2003.tb01386.x] [PMID: 14611673]
[36]
Neufert, C.; Becker, C.; Neurath, M.F. An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression. Nat. Protoc., 2007, 2(8), 1998-2004.
[http://dx.doi.org/10.1038/nprot.2007.279] [PMID: 17703211]
[37]
Sliva, D.; Loganathan, J.; Jiang, J.; Jedinak, A.; Lamb, J.G.; Terry, C.; Baldridge, L.A.; Adamec, J.; Sandusky, G.E.; Dudhgaonkar, S. Mushroom Ganoderma lucidum prevents colitis-associated carcinogenesis in mice. PLoS One, 2012, 7(10)e47873
[http://dx.doi.org/10.1371/journal.pone.0047873] [PMID: 23118901]
[38]
Sohn, O.S.; Fiala, E.S.; Requeijo, S.P.; Weisburger, J.H.; Gonzalez, F.J. Differential effects of CYP2E1 status on the metabolic activation of the colon carcinogens azoxymethane and methylazoxymethanol. Cancer Res., 2001, 61(23), 8435-8440.
[PMID: 11731424]
[39]
Okayasu, I.; Hatakeyama, S.; Yamada, M.; Ohkusa, T.; Inagaki, Y.; Nakaya, R. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology, 1990, 98(3), 694-702.
[http://dx.doi.org/10.1016/0016-5085(90)90290-H] [PMID: 1688816]
[40]
Waly, M.I.; Al-Rawahi, A.S.; Al Riyami, M.; Al-Kindi, M.A.; Al-Issaei, H.K.; Farooq, S.A.; Al-Alawi, A.; Rahman, M.S. Amelioration of azoxymethane induced-carcinogenesis by reducing oxidative stress in rat colon by natural extracts. BMC Complement. Altern. Med., 2014, 14, 60.
[http://dx.doi.org/10.1186/1472-6882-14-60] [PMID: 24533833]
[41]
De Robertis, M.; Massi, E.; Poeta, M.L.; Carotti, S.; Morini, S.; Cecchetelli, L.; Signori, E.; Fazio, V.M. The AOM/DSS murine model for the study of colon carcinogenesis: From pathways to diagnosis and therapy studies. J. Carcinog., 2011, 10, 9.
[http://dx.doi.org/10.4103/1477-3163.78279] [PMID: 21483655]
[42]
Kumar, S.K.; Suresh, S.M.; Kumar, A.; Kalaiselvi, P. Bioactive compounds, radical scavenging, antioxidant properties and FTIR spectroscopy study of Morinda citrifolia fruit extracts. Int. J. Curr. Microbiol. Appl. Sci., 2014, 3, 28-42.
[43]
Selek, S.; Koyuncu, I.; Caglar, H.G.; Bektas, I.; Yilmaz, M.A.; Gonel, A.; Akyuz, E. The evaluation of antioxidant and anticancer effects of Lepidium Sativum Subsp Spinescens L. methanol extract on cancer cells. Cell. Mol. Biol., 2018, 64(3), 72-80, 72-80.,
[http://dx.doi.org/10.14715/cmb/2018.64.3.12 ] [PMID: 29506633]
[44]
Kassie, F.; Rabot, S.; Uhl, M.; Huber, W.; Qin, H.M.; Helma, C.; Schulte-Hermann, R. KnasmA1/4ller, S. Chemoprotective effects of garden cress (Lepidium sativum) and its constituents towards 2-amino-3-methyl-imidazo[4,5-f]quinoline (IQ)-induced genotoxic effects and colonic preneoplastic lesions. Carcinogenesis, 2002, 23(7), 1155-1161.
[http://dx.doi.org/10.1093/carcin/23.7.1155] [PMID: 12117773]
[45]
Moreno, R.L.; Goosen, T.; Kent, U.M.; Chung, F.L.; Hollenberg, P.F. Differential effects of naturally occurring isothiocyanates on the activities of cytochrome P450 2E1 and the mutant P450 2E1 T303A. Arch. Biochem. Biophys., 2001, 391(1), 99-110.
[http://dx.doi.org/10.1006/abbi.2001.2390] [PMID: 11414690]
[46]
van Breda, S.G.; de Kok, T.M.; van Delft, J.H. Mechanisms of colorectal and lung cancer prevention by vegetables: a genomic approach. J. Nutr. Biochem., 2008, 19(3), 139-157.
[http://dx.doi.org/10.1016/j.jnutbio.2007.04.002] [PMID: 17651960]
[47]
Greenblatt, M.S.; Bennett, W.P.; Hollstein, M.; Harris, C.C. Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis. Cancer Res., 1994, 54(18), 4855-4878.
[PMID: 8069852]
[48]
Hussien, N.A. Absence of p53 gene mutations in mice colon pre-cancerous stage induced by o-nitrotoluene. J. Cancer Res. Ther., 2014, 10(4), 1008-1012.
[http://dx.doi.org/10.4103/0973-1482.140773] [PMID: 25579545]
[49]
Minamoto, T.; Buschmann, T.; Habelhah, H.; Matusevich, E.; Tahara, H.; Boerresen-Dale, A.L.; Harris, C.; Sidransky, D.; Ronai, Z. Distinct pattern of p53 phosphorylation in human tumors. Oncogene, 2001, 20(26), 3341-3347.
[http://dx.doi.org/10.1038/sj.onc.1204458] [PMID: 11423984]
[50]
Alqahtani, F.Y.; Aleanizy, F.S.; Mahmoud, A.Z.; Farshori, N.N.; Alfaraj, R.; Al-Sheddi, E.S.; Alsarra, I.A. Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil. Saudi J. Biol. Sci., 2019, 26(5), 1089-1092.
[http://dx.doi.org/10.1016/j.sjbs.2018.05.007] [PMID: 31303845]
[51]
Sat, I. Gr.; Yildirim, E.; Turan, M.; Demirbas, M. Antioxidant and nutritional characteristics of garden cress (Lepidium sativum). Acta Sci. Pol. Hortorum Cultus, 2013, 12, 173-179.

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