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Current Pharmaceutical Analysis

Editor-in-Chief

ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

Research Article

Metabolic Changes in Mouse Plasma after Acute Diquat Poisoning by UPLC-MS/MS

Author(s): Lianguo Chen, Zuoquan Zhong, Jiawen Liu, Congcong Wen, Yongxi Jin and Xianqin Wang*

Volume 17, Issue 7, 2021

Published on: 24 June, 2020

Page: [903 - 907] Pages: 5

DOI: 10.2174/1573412916999200624160304

Price: $65

Abstract

Introduction: Diquat is a fast-acting contact herbicide and plant dehydrating agent. The oral lethal dose 50 (LD50) of diquat in mice is about 125 mg/kg. The purpose of this study is to research the metabolomics in mouse plasma after acute diquat poisoning.

Methods: The mice were divided into two groups (the control group and acute diquat poisoning group). The control group was given normal saline by gavage. The acute diquat poisoning group was given 50 mg/kg diquat. UPLC-MS/MS was used to determinate the small molecule organic acid in mouse plasma.

Results: Compared to the control group, the L-lysine, Adenine, L-Alanine, L-Valine, Lactic acid, Inosine, Adenosine, L-Tryptophan, L-Tyrosine, L-Arginine, L-Phenylalanine, L-Methionine, Citric acid, Fructose, L-Glutamine, Malic acid, L-Aspartic acid and Pyruvic acid increased in the acute diquat poisoning group (p<0.05); while the L-Histidine decreased (p<0.05).

Conclusion: The results of metabolites increased or decreased, indicating that acute diquat poisoning induced amino acid metabolism and energy metabolism perturbations in mice.

Keywords: Diquat, plasma, UPLC-MS/MS, amino acid, mouse, metabolomics.

Graphical Abstract
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