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Current Nutraceuticals

Editor-in-Chief

ISSN (Print): 2665-9786
ISSN (Online): 2665-9794

Research Article

The Supremacy of Synergism: A Comparison of Anticancer Activity of Rhizome Extract of Bistorta Amplexicaulis and Gallic Acid in Cancer Cell Lines and Primary Cells

Author(s): Salma Batool, M. Javaid Asad, Muhammad Arshad, Rahman Shah Zaib Saleem* and Muhammad Sheeraz Ahmad*

Volume 2, Issue 1, 2021

Published on: 17 February, 2020

Page: [21 - 26] Pages: 6

DOI: 10.2174/2665978601666200218090845

Abstract

Background: Bistorta amplexicaulis is a seasonal herb with several folkloric uses. The plant extract has been shown to possess various activities including antioxidant, anticancer, anti-bacterial, anti-fungal, cardio-protective, and anti-atherosclerosis activities.

Objective: The aim of the study was to quantify the activity of the plant extract and relate it to the activity of the isolated compound, gallic acid.

Methods: Initially, the plant was extracted, then, the activity of the extract was compared with its constituent, gallic acid. After this, the cytotoxic potential of the two against human liver cancer cell line (HepG2), breast cancer cell line (MCF-7) and human umbilical vein endothelial cells (HUVEC) was evaluated through MTS assay.

Results: The extract had better activity against HepG2 as compared to gallic acid (IC50 29μg/mL vs 37μg/mL). It also provided a better therapeutic window by having lower toxicity for HUVEC cells than gallic acid (IC50 63μg/mL vs 47μg/mL), suggesting the use of the extract over the purified gallic acid for these cells. We also performed the fluorescence study of the rhizome extract in ethanol (REE), methanol (REM), 80% ethanol (80RE), 80% methanol, (80RM) and acetone (RAC). The highest intensity of fluorescence was found in REE with excitation at 394 nm and emission at 421nm.

Conclusion: The comparison of gallic acid with ethanolic rhizome extract of B. amplexicaulis reveals important insights about utilizing the plant extract over purified gallic acid. The ethanolic extract also has the potential to be used as an autofluorescent drug during in vitro and in vivo studies.

Keywords: Bistorta amplexicaulis, anticancer activity, HepG2; MCF-7, gallic acid, HUVEC.

Graphical Abstract
[1]
Aung, T.N.; Qu, Z.; Kortschak, R.D.; Adelson, D.L. Understanding the effectiveness of natural compound mixtures in cancer through their molecular mode of action. Int. J. Mol. Sci., 2017, 18(3), 656-676.
[http://dx.doi.org/10.3390/ijms18030656] [PMID: 28304343]
[2]
Singh, C.K.; George, J.; Ahmad, N. Resveratrol-based combinatorial strategies for cancer management. Ann. N. Y. Acad. Sci., 2013, 1290(1), 113-121.
[http://dx.doi.org/10.1111/nyas.12160] [PMID: 23855473]
[3]
Sawyers, C.L. Cancer: Mixing cocktails. Nature, 2007, 449(7165), 993-996.
[http://dx.doi.org/10.1038/449993a] [PMID: 17960228]
[4]
HemaIswarya S.; Doble, M. Potential synergism of natural products in the treatment of cancer. Phytother. Res., 2006, 20(4), 239-249.
[http://dx.doi.org/10.1002/ptr.1841] [PMID: 16557604]
[5]
Piccolo, M.T.; Menale, C.; Crispi, S. Combined anticancer therapies: an overview of the latest applications. Anticancer. Agents Med. Chem., 2015, 15(4), 408-422.
[http://dx.doi.org/10.2174/1871520615666150113123039] [PMID: 25584691]
[6]
Houghton, P.J. Use of small scale bioassays in the discovery of novel drugs from natural sources. Phytother. Res., 2000, 14(6), 419-423.
[http://dx.doi.org/10.1002/1099-1573(200009)14:6<419:AID-PTR720>3.0.CO;2-2] [PMID: 10960894]
[7]
Schmid, B.; Lüdtke, R.; Selbmann, H.K.; Kötter, I.; Tschirdewahn, B.; Schaffner, W.; Heide, L. Efficacy and tolerability of a standardized willow bark extract in patients with osteoarthritis: randomized placebo-controlled, double blind clinical trial. Phytother. Res., 2001, 15(4), 344-350.
[http://dx.doi.org/10.1002/ptr.981] [PMID: 11406860]
[8]
Cantelli-Forti, G.; Maffei, F.; Hrelia, P.; Bugamelli, F.; Bernardi, M.; D’Intino, P.; Maranesi, M.; Raggi, M.A. Interaction of licorice on glycyrrhizin pharmacokinetics. Environ. Health Perspect., 1994, 102(Suppl. 9), 65-68.
[http://dx.doi.org/10.1289/ehp.94102s965] [PMID: 7698088]
[9]
Kostoudi, S.; Koutousi, C.; Mironidou-Tzouveleki, M. Cannabinoids in clinical practice.Ep Klin. Farmakol. kai Farmakokinet, 2005, 23, 265-72.,
[10]
Kimura, M.; Kimura, I.; Guo, X.; Luo, B.; Kobayashi, S. Combined effects of Japanese‐Sino medicine ‘Kakkon‐to‐ka‐senkyu‐shin’i’ and its related combinations and component drugs on adjuvant‐induced inflammation in mice. Phytother. Res., 1992, 6, 209-216.
[http://dx.doi.org/10.1002/ptr.2650060409]
[11]
Williamson, E.M. Synergy and other interactions in phytomedicines. Phytomedicine, 2001, 8(5), 401-409.
[http://dx.doi.org/10.1078/0944-7113-00060] [PMID: 11695885]
[12]
Batool, S.; Ahmed, M.S. Bistorta amplexicaulis: A brief insight to its ethnobotany. J. Bioresour. Manag., 2017, 3, 2-4.
[13]
Qureshi, R.A.; Gilani, S.A.; Ashraf, M. Ethnobotanical studies with special reference to plants phenology at Sudhan Gali and Ganga Chotti Hills (District Bagh, A.K.). Electron J. Environ. Agric. Food Chem., 2007, 6, 2207-2215.
[14]
Adnan, M.; Hölscher, D. Medicinal plant abundance in degraded and reforested sites in northwest Pakistan. Mt. Res. Dev., 2010, 30, 25-32.
[http://dx.doi.org/10.1659/MRD-JOURNAL-D-09-00017.1]
[15]
Bhatt, A.; Rawal, R.S.; Dhar, U. Ecological features of a critically rare medicinal plant, Swertia chirayita, in Himalaya. Plant Species Biol., 2006, 21, 49-52.
[http://dx.doi.org/10.1111/j.1442-1984.2006.00150.x]
[16]
Sharma, P.K.; Chauhan, N.S.; Lal, B. Observations on the traditional phytotherapy among the inhabitants of Parvati valley in western Himalaya, India. J. Ethnopharmacol., 2004, 92(2-3), 167-176.
[http://dx.doi.org/10.1016/j.jep.2003.12.018] [PMID: 15137998]
[17]
Dong, X.; Fu, J.; Yin, X.; Li, X.; Wang, B.; Cao, S. Pharmacological and other bioactivities of the genus Polygonum - A review. Trop. J. Pharm. Res., 2014, 13, 1749-1759.
[18]
Srivastava, T.N. Wild edible plants of Jammu & Kashmir state - An ethno-botanical study. Anc. Sci. Life, 1988, 7(3-4), 201-206.
[PMID: 22557615]
[19]
Khan, M.A.; Khan, M.A.; Hussain, M. Medicinal Plants Used in Folk Recipes by the Inhabitants of Himalayan Region Poonch Valley Azad Kashmir (Pakistan). J. Basic Appl. Sci., 2012, 8, 35-45.
[http://dx.doi.org/10.6000/1927-5129.2012.08.01.21]
[20]
Batool, S.; Gulfraz, M.; Akram, A.; Naqvi, S.S.; Haq, I.; Mirza, B.; Ahmad, M.S. Evaluation of antioxidant potential and HPLC based identification of phenolics in Polygonum amplexicaule extract and its fractions. Pak. J. Pharm. Sci., 2015, 28(2), 431-435.
[PMID: 25730800]
[21]
Ahmad, M.S.; Naqvi, S.M.S.; Mushtaq, S.; Ramzan, F.; Sami, A.; Batool, S. Phytochemical and biological evaluation of Polygonum amplexicaule rhizome extract. Can. J. Pure Appl. Sci., 2013, 7, 2491-2498.
[22]
Tantry, M.A.; Radwan, M.M.; Akbar, S.; Khan, I.A. 5, 6-Dihydropyranobenzopyrone: a previously undetermined antioxidant isolated from Polygonum amplexicaule. Chin. J. Nat. Med., 2012, 10(1), 28-31.
[http://dx.doi.org/10.1016/S1875-5364(12)60006-5] [PMID: 23302526]
[23]
Intisar, A.; Kiazolu, J.B.; Wang, Y.; Zhang, L.; Zhang, W. effect of mobile phase composition and pH on HPLC separation of rhizome of Polygonum bistorta. J. Liq. Chromatogr. Relat. Technol., 2012, 35, 977-987.http://www.tandfonline.com/doi/abs/10.1080/10826076.2011.615089 [Internet].
[http://dx.doi.org/10.1080/10826076.2011.615089]
[24]
Tantry, M.A.; Rahman, A.A. Amplexicine, an antioxidant flavan-3-ol from Polygonum amplexicaule. Nat. Prod. Commun., 2011, 6(11), 1597-1598.
[http://dx.doi.org/10.1177/1934578X1100601111] [PMID: 22224270]
[25]
Lieberman, M.M.; Patterson, G.M.; Moore, R.E. In vitro bioassays for anticancer drug screening: effects of cell concentration and other assay parameters on growth inhibitory activity. Cancer Lett., 2001, 173(1), 21-29.
[http://dx.doi.org/10.1016/S0304-3835(01)00681-4] [PMID: 11578805]
[26]
Drabent, R.; Pliszka, B.; Olszewska, T. Fluorescence properties of plant anthocyanin pigments. I. Fluorescence of anthocyanins in Brassica oleracea L. extracts. J. Photochem. Photobiol. B, 1999, 50, 53-58.
[http://dx.doi.org/10.1016/S1011-1344(99)00070-6]
[27]
Dai, J.; Mumper, R.J. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 2010, 15, 7313-7352.
[28]
Ferreira, G.; Hernandez-Martinez, A.R.; Pool, H.; Molina, G.; Cruz-Soto, M.; Luna-Barcenas, G.; Estevez, M. Synthesis and functionalization of silica-based nanoparticles with fluorescent biocompounds extracted from Eysenhardtia polystachya for biological applications. Mater. Sci. Eng. C, 2015, 57, 49-57.
[http://dx.doi.org/10.1016/j.msec.2015.07.012] [PMID: 26354239]
[29]
Talamond, P.; Verdeil, J.L.; Conéjéro, G. Secondary metabolite localization by autofluorescence in living plant cells. Molecules, 2015, 2, 5024-5037.
[30]
Dinoiu, V.; Voicescu, M.; Lungu, L.; Savoiu, M.; Vasilescu, M. Spectroscopic Study on the Riboflavin-Plant Extracts Interaction. Rev. de Chim., 2011, 11, 1-4.

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