Background: This study determined the effect of Biochanin A (BCA) on isoproterenol (ISO) induced Myocardial Infarction (MI) in male Wistar rats.
Methods: Animals (weighing 150-180 g) were divided into four groups, with six animals in each group and pretreated with BCA (10mg/kg Body Weight [BW]) and ɑ-tocopherol (60mg/kg BW) for 30 days; and ISO (20mg/kg BW) was administrated subcutaneously on the 31st and 32nd day.
Results: ISO-induced MI rats demonstrated the significant elevation of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, lactate dehydrogenase, creatine kinase-MB and cardiac troponin; however, concomitant pretreatment with BCA protected the rats from cardiotoxicity caused by ISO. Activities of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase significantly reduced in the heart with ISO-induced MI. Pretreatment with BCA produced a marked reversal of these antioxidant enzymes related to MI-induced by ISO.
Conclusion: In conclusion, this study suggested that BCA exerts cardioprotective effects through modulating lipid peroxidation, enhancing antioxidants, and detoxifying enzyme systems.
[http://dx.doi.org/10.1089/jmf.2013.2795] [PMID: 24325453]
[http://dx.doi.org/10.1016/j.ijcard.2011.09.005] [PMID: 21962802]
[http://dx.doi.org/10.1155/2011/635058] [PMID: 21437195]
[http://dx.doi.org/10.1016/j.nutres.2008.03.009] [PMID: 19083447]
[http://dx.doi.org/10.1111/j.1749-6632.1972.tb27851.x] [PMID: 4572176]
[http://dx.doi.org/10.1016/S0735-1097(00)00804-4] [PMID: 10987628]
[http://dx.doi.org/10.1373/clinchem.2009.133546] [PMID: 20378771]