Generic placeholder image

Current Pharmaceutical Design

Editor-in-Chief

ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

Research Article

A Single Medical Marker for Diagnosis of Methamphetamine Addiction - DNA Methylation of SHATI/NAT8L Promoter Sites from Patient Blood

Author(s): Kusui Yuka, Daisuke Nishizawa, Junko Hasegawa, Kyosuke Uno, Hajime Miyanishi, Hiroshi Ujike, Norio Ozaki, Toshiya Inada, Nakao Iwata, Ichiro Sora, Masaomi Iyo, Mitsuhiko Yamada, Naoki Kondo, Moo-Jun Won, Nobuya Naruse, Kumi Uehara-Aoyama, Kazutaka Ikeda and Atsumi Nitta*

Volume 26, Issue 2, 2020

Page: [260 - 264] Pages: 5

DOI: 10.2174/1381612826666200110111703

Price: $65

Abstract

Background: Methamphetamine (METH) is one of the most widely distributed psychostimulants worldwide. Despite active counter measures taken by different countries, neither overall usage of METH nor the frequency of repeat users has reduced over the past decade. METH induces abuse and dependence as it acts on the central nervous system and temporarily stimulates the brain. The recidivism rate for abuse of stimulants in Japan is very high and therefore prevention of repeated usage is paramount. However, we lack information about the relationship between METH users and genomic changes in humans in Japan, which would provide important information to aid such efforts.

Objective: Shati/Nat8l is a METH-inducible molecule and its overexpression has protective effects on the brain upon METH usage. Here we investigated the effect of METH usage on DNA methylation rates at the promoter site of SHATI/NAT8L. We used DNA samples from human METH users, who are usually difficult to recruit in Japan.

Methods: We measured DNA methylation at SHATI/NAT8L promoter sites by pyrosequencing method using 193 samples of METH users and 60 samples of healthy subjects. In this method, DNA methylation is measured by utilizing the property that only non-methylated cytosine changes to urasil after bisulfite conversion.

Results: We found that the rate of DNA methylation at six CpG islands of SHATI/NAT8L promoter sites is significantly higher in METH users when compared to healthy subjects.

Conclusion: These results suggest that the DNA methylation rate of SHATI/NAT8L promotor regions offers a new diagnostic method for METH usage.

Keywords: Methamphetamine (METH), addiction, epigenetics, DNA methylation, SHATI/NAT8L, diagnosis.

[1]
World Drug Report UNODC. United Nations Office on Drugs and Crime (ed.) Vienna 2018. Available at: https://www.unodc.org/doc/wdr2018/WDR_2018_Press_ReleaseENG.PDF
[2]
Chomchai C, Chomchai S. Global patterns of methamphetamine use. Curr Opin Psychiatry 2015; 28(4): 269-74.
[http://dx.doi.org/10.1097/YCO.0000000000000168] [PMID: 26001916]
[3]
Koob GF. Drugs of abuse: anatomy, pharmacology and function of reward pathways. Trends Pharmacol Sci 1992; 13(5): 177-84.
[http://dx.doi.org/10.1016/0165-6147(92)90060-J] [PMID: 1604710]
[4]
Wise R. Neurobiology of addiction. Curr Opin Neurobiol 1996; 6(2): 243-51.
[http://dx.doi.org/10.1016/S0959-4388(96)80079-1]
[5]
Nestler EJ, Lüscher C. The molecular basis of drug addiction: linking epigenetic to synaptic and circuit mechanisms. Neuron 2019; 102(1): 48-59.
[http://dx.doi.org/10.1016/j.neuron.2019.01.016] [PMID: 30946825]
[6]
Ministry of Health. Labor and Welfare in Japan. Available at: https://www.mhlw.go.jp/bunya/iyakuhin/yakubuturanyou/dl/pamphlet_04.pdf
[7]
González B, Jayanthi S, Gomez N, et al. Repeated methamphetamine and modafinil induce differential cognitive effects and specific histone acetylation and DNA methylation profiles in the mouse medial prefrontal cortex. Prog Neuropsychopharmacol Biol Psychiatry 2018; 82: 1-11.
[http://dx.doi.org/10.1016/j.pnpbp.2017.12.009] [PMID: 29247759]
[8]
Uno K, Kikuchi Y, Iwata M, et al. Decreased DNA methylation in the Shati/Nat8l promoter in both patients with schizophrenia and a methamphetamine-induced murine model of schizophrenia-like phenotype. PLoS One 2016; 11(6), e0157959
[http://dx.doi.org/10.1371/journal.pone.0157959] [PMID: 27348532]
[9]
Niwa M, Nitta A, Mizoguchi H, et al. A novel molecule “shati” is involved in methamphetamine-induced hyperlocomotion, sensitization, and conditioned place preference. J Neurosci 2007; 27(28): 7604-15.
[http://dx.doi.org/10.1523/JNEUROSCI.1575-07.2007] [PMID: 17626222]
[10]
Uno K, Miyazaki T, Sodeyama K, Miyamoto Y, Nitta A. Methamphetamine induces Shati/Nat8L expression in the mouse nucleus accumbens via CREB- and dopamine D1 receptor-dependent mechanism. PLoS One 2017; 12(3), e0174196
[http://dx.doi.org/10.1371/journal.pone.0174196] [PMID: 28319198]
[11]
Haddar M, Uno K, Azuma K, Muramatsu SI, Nitta A. Inhibitory effects of Shati/Nat8l overexpression in the medial prefrontal cortex on methamphetamine-induced conditioned place preference in mice. Addict Biol 2019. In press
[http://dx.doi.org/10.1111/adb.12749] [PMID: 30950164]
[12]
Nishizawa D, Fukuda K, Kasai S, et al. Genome-wide association study identifies a potent locus associated with human opioid sensitivity. Mol Psychiatry 2014; 19(1): 55-62.
[http://dx.doi.org/10.1038/mp.2012.164] [PMID: 23183491]
[13]
Ujike H, Harano M, Inada T, et al. Nine- or fewer repeat alleles in VNTR polymorphism of the dopamine transporter gene is a strong risk factor for prolonged methamphetamine psychosis. Pharmacogenomics J 2003; 3(4): 242-7.
[http://dx.doi.org/10.1038/sj.tpj.6500189] [PMID: 12931138]
[14]
Fusco G, Amoroso MG, Gesualdi Montesano N, Viscardi M. Development of a pyrosequencing assay for the typing of alphaherpesviruses. MethodsX 2015; 2: 47-52.
[http://dx.doi.org/10.1016/j.mex.2015.01.001] [PMID: 26150971]
[15]
Alderborn A, Kristofferson A, Hammerling U. Determination of single-nucleotide polymorphisms by real-time pyrophosphate DNA sequencing. Genome Res 2000; 10(8): 1249-58.
[http://dx.doi.org/10.1101/gr.10.8.1249] [PMID: 10958643]
[16]
Gillio-Tos A, Fiano V, Grasso C, et al. New technologies for cervical cancer screening (NTCC) working group. Assessment of viral methylation levels for high risk HPV types by newly designed consensus primers PCR and pyrosequencing. PLoS One 2018; 13(3), e0194619
[http://dx.doi.org/10.1371/journal.pone.0194619] [PMID: 29579066]
[17]
Sabrini S, Wang GY, Lin JC, Ian JK, Curley LE. Methamphetamine use and cognitive function: a systematic review of neuroimaging research. Drug Alcohol Depend 2019; 194: 75-87.
[http://dx.doi.org/10.1016/j.drugalcdep.2018.08.041] [PMID: 30414539]
[18]
Moszczynska A, Callan SP. Molecular, behavioral, and physiological consequences of methamphetamine neurotoxicity: implications for treatment. J Pharmacol Exp Ther 2017; 362(3): 474-88.
[http://dx.doi.org/10.1124/jpet.116.238501] [PMID: 28630283]
[19]
Dong N, Zhu J, Han W, et al. Maternal methamphetamine exposure causes cognitive impairment and alteration of neurodevelopment-related genes in adult offspring mice. Neuropharmacology 2018; 140: 25-34.
[http://dx.doi.org/10.1016/j.neuropharm.2018.07.024] [PMID: 30048643]
[20]
Cui D, Xu X. DNA Methyltransferases, DNA methylation, and age-associated cognitive function. Int J Mol Sci 2018; 19(5), E1315
[http://dx.doi.org/10.3390/ijms19051315] [PMID: 29710796]
[21]
Sanger F, Donelson JE, Coulson AR, Kössel H, Fischer D. Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA. Proc Natl Acad Sci USA 1973; 70(4): 1209-13.
[http://dx.doi.org/10.1073/pnas.70.4.1209] [PMID: 4577794]
[22]
Sanger F, Coulson AR. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol 1975; 94(3): 441-8.
[http://dx.doi.org/10.1016/0022-2836(75)90213-2] [PMID: 1100841]
[23]
Nishida M, Yashiki M, Namera A, Kimura K. Single hair analysis of methamphetamine and amphetamine by solid phase microextraction coupled with in matrix derivatization. J Chromatogr B Analyt Technol Biomed Life Sci 2006; 842(2): 106-10.
[http://dx.doi.org/10.1016/j.jchromb.2006.07.039] [PMID: 16971192]

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy