Hydrogen Production by Thermophilic Fermentation
Pp. 137-159 (23)
Ed W. J. van Niel, Karin Willquist, Ahmad A. Zeidan, Truus de Vrije, Astrid E. Mars and Pieternel A. M. Claassen
Of the many ways hydrogen can be produced, this chapter focuses on biological hydrogen
production by thermophilic bacteria and archaea in dark fermentations. The thermophiles are held as
promising candidates for a cost-effective fermentation process, because of their relatively high yields
and broad substrate palette. Yet many challenges remain to be faced, including improving productivity,
tolerance to high osmolality and growth inhibitors, and reactor configuration. This review consolidates
current insights in the quest for high yields and productivities within thermophilic hydrogen production.
Important is to understand how environmental parameters affect the redox- and energy metabolism of
the microorganism(s) involved. This knowledge is required for designing an optimal bioreactor
configuration and operation.
Central carbon metabolism, hydrogenase, hydrogen productivity, hydrogen yield, hydrogen
partial pressure, hydrolysate, medium composition, osmolality, physiology, reactor design,
Lund University, Lund, Sweden and Food and Biobased Research, Wageningen, Netherlands