Current Stem Cell Research & Therapy

Anthony Atala  
Wake Forest University School of Medicine,
Medical Center Boulevard
Winston Salem, NC 27157
USA

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Expression of the Two Pore Domain Potassium Channel TREK-1 in Human Intervertebral Disc Cells

Author(s): Pankaj Sharma, Stephen Hughes, Alicia El Haj, Nicola Maffulli.

Abstract:

Potassium channels play a major role in intracellular homeostasis and regulation of cell volume. Intervertebral disc cells respond to mechanical loading in a complex manner. Mechanical loading may play a role in disc degeneration. Lumbar intervertebral disc samples from 5 patients (average age: 47 years, range: 25-64 years) were used for this study, investigating cells from the nucleus pulposus and the annulus fibrosus duplicate samples to determine RNA expression and protein expression. Analysis of mRNA expression by RT-PCR demonstrated that TREK 1 was expressed by nucleus pulposus (n=5) and annulus fibrosus (n=5) cells. Currently, TREK-1 is the only potassium channel known to be activated by intracellular acidosis, and responds to mechanical and chemical stimuli. Whilst the precise role of potassium channels in cellular homeostasis remains to be determined, TREK-1 may be important to protect disc cells against ischaemic damage, and subsequent disc degeneration, and may also play a role in effecting mechanotransduction. Further research is required to fully elucidate the role of the TREK-1 ion channel in intervertebral disc cells.

Keywords: Annulus fibrosus, intervertebral disc, mechanotransduction, nucleus pulposus, potassium channel, TREK-1, disc degeneration, RNA, mechanical loading, intracellular homeostasis

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Article Details

VOLUME: 7
ISSUE: 4
Year: 2012
Page: [266 - 271]
Pages: 6
DOI: 10.2174/157488812800793072
Price: $58