Antibodies Applications and New Developments

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Antibodies Applications and New Developments is an overview of the current developments of techniques and methods relating to immunodiagnostics and immunoanalysis. This eBook also deals with ...
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Principles and Applications of Immunoaffinity Chromatography

Pp. 156-174 (19)

Abby J. Jackson, Matthew R. Sobansky and David S. Hage

Abstract

Immunoaffinity Chromatography (IAC) is a type of liquid chromatography that uses the highly specific binding of antibodies for the retention of a target. This approach can be used in a myriad of techniques for the selective purification, concentration and/or analysis of target compounds. This chapter discusses the basic components of an IAC method, including the support, stationary phase, immobilization technique, and proper sample application and elution conditions. Several applications of IAC are also considered. These applications include immunoextraction, immunodepletion, chromatographic immunoassays, and post-column immunodetection. A wide range of targets that have been isolated or measured by these techniques are listed. Related methods based on antibody mimics are also discussed.

Keywords:

Antibodies, Immunoaffinity Chromatography

Affiliation:

Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA