Antibodies Applications and New Developments

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Antibodies Applications and New Developments is an overview of the current developments of techniques and methods relating to immunodiagnostics and immunoanalysis. This eBook also deals with ...
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Production of Antibodies for Immunoanalytical Methods

Pp. 29-47 (19)

Ludek Eyer and Milan Franek

Abstract

Fundamental immunological principles and factors influencing production strategies for antibodies used as reagents in analytical and diagnostic methods are highlighted. A role of the peptideprotein design for an antibody formation, covering particularly epitope selection, peptide and protein chemistry, carrier-protein conjugation including multiple antigen protein approach are described. Special attention is paid to hapten design and production of antibodies against molecules having lowmolecular character. Critical stages in polyclonal and monoclonal antibody development as well as practical hints for their production are discussed.

Keywords:

Antibodies, Production, Hapten, Immunogens

Affiliation:

Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic