Enhanced Mechanical Properties of Rabbit Flexor Tendons in Response to Intratendinous Injection of Adipose Derived Stromal Vascular Fraction
Introduction: Tendon injuries are notorious for slow and functionally inferior healing. It is claimed that cellbased
therapy would result in faster and more efficient healing of injured tissues with less postoperative complications.
Given the limitations associated with ex vivo cellular expansion, we tried to evaluate the possible effects of intratendinous
injection of adipose derived stromal vascular fraction on mechanical properties of tendon repair.
Methods: The model of injury was complete sharp transection of rabbit deep digital flexor tendon followed by primary suture
repair and an intratendinous injection of either allogeneic stromal vascular fraction or placebo. Tendons were harvested
at three and eight weeks after surgery.
Results: The results of mechanical testing showed the treatment caused significant increase in ultimate and yield loads,
stress, and energy absorption of repairs compared to controls at both time points. Also, improvement in terms of strain and
stiffness were detected at the eighth week in treatments.
Discussion: In comparison with the result of previous studies using cultured mesenchymal stem cells from bone marrow
or adipose tissue; the improved mechanical properties observed in the present study suggest that choosing stromal vascular
fraction as a readily accessible and instant source of multipotent cells instead of expensive and long-lasting culture expansion
may seem more favorable in cell based therapy for tendon injuries. The mechanical functionality of the repairs
observed in the present study encourages further investigations into the use of stromal vascular fraction for the repair of
Keywords: Adipose, intratendinous, mechanical properties, rabbit, stromal cells, tendon
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