The relevance of anti-human leucocyte antigen (anti-HLA) antibodies in the outcome of organ transplantation has been recognized in the past 40 years. Detection of these antibodies in sensitized recipients plays a pivotal role in this issue. Flow cytometry analysis is one of the choice cell-based techniques together with the pioneer complementdependent cytotoxicity crossmatch. Here, we discuss the advantages and limits of these cell-based assays and their use in the clinical laboratory of immunology of transplantation. Moreover, we compared this approach to other beads-based detection assays introduced more recently in the laboratory practice.
Keywords: Anti-HLA antibodies, beads-based assay, cell-based assay, complement-dependent cytotoxicity crossmatch, flow cytometry crossmatch, luminex crossmatch, Luminex Single Antigen technology, monoclonal antibodies, organ transplantation, solid-phase assay, virtual crossmatch, XM-ONE
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