Abstract
Bauninia forficata is trivially known as cow paw, and popularly used in Brazil for treatment of diabetes mellitus. Denominated baupain a cysteine proteinase was purified from B. forficata leaves. In this study, we investigated the baupain effect on aggregation of isolated human platelets in vitro and the results show that baupain hinders thrombin - but not ADP- and collagen- induced platelet aggregation. With synthetic quenched-fluorescent peptides, the kinetics of the cleavage site of human proteinase-activated receptor 1 / 2 / 3 and 4 [PAR-1 / 2 / 3 and 4] by baupain was determined. In conclusion, similar to bromelain and papain, baupain hinders human platelets aggregation, probably through an unspecific cleavage in the Phe-Leu bond of PAR1.
Keywords: Bauhinia, cysteine proteinase, plant enzyme, platelet aggregation, thrombin, collagen, human proteinase-activated receptor, baupain, Phe-Leu
Protein & Peptide Letters
Title:Baupain, A Plant Cysteine Proteinase That Hinders Thrombin-Induced Human Platelet Aggregation
Volume: 19 Issue: 4
Author(s): Sheila S. Andrade, M. C.C. Silva, I. E. Gouvea, M. Y. Kondo, M. A. Juliano, M. U. Sampaio and Maria Luzia Oliva
Affiliation:
Keywords: Bauhinia, cysteine proteinase, plant enzyme, platelet aggregation, thrombin, collagen, human proteinase-activated receptor, baupain, Phe-Leu
Abstract: Bauninia forficata is trivially known as cow paw, and popularly used in Brazil for treatment of diabetes mellitus. Denominated baupain a cysteine proteinase was purified from B. forficata leaves. In this study, we investigated the baupain effect on aggregation of isolated human platelets in vitro and the results show that baupain hinders thrombin - but not ADP- and collagen- induced platelet aggregation. With synthetic quenched-fluorescent peptides, the kinetics of the cleavage site of human proteinase-activated receptor 1 / 2 / 3 and 4 [PAR-1 / 2 / 3 and 4] by baupain was determined. In conclusion, similar to bromelain and papain, baupain hinders human platelets aggregation, probably through an unspecific cleavage in the Phe-Leu bond of PAR1.
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S. Andrade Sheila, C.C. Silva M., E. Gouvea I., Y. Kondo M., A. Juliano M., U. Sampaio M. and Luzia Oliva Maria, Baupain, A Plant Cysteine Proteinase That Hinders Thrombin-Induced Human Platelet Aggregation, Protein & Peptide Letters 2012; 19 (4) . https://dx.doi.org/10.2174/092986612799789305
DOI https://dx.doi.org/10.2174/092986612799789305 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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