Detection of Damage of Biomolecules by Free Radicals and Related Reactive Species
Pp. 295-306 (12)
Y. Robert Li
Lipids, proteins, and nucleic acids are major cellular targets of reactive oxygen and nitrogen
species (ROS/RNS). Oxidative damage to these cellular constituents has been increasingly recognized as a
significant pathophysiological event leading to many disease processes. Thus, determination of ROS/RNSelicited
damage to lipids, proteins, and nuclear acids is crucial for investigating the oxidative mechanisms of
human diseases and assessing the efficacy of antioxidant-based therapies. This chapter first introduces the
general experimental approaches to assessing the effects of ROS/RNS on cellular biomolecules and then
describes the commonly used methods to determine ROS/RNS-mediated biological damage, with an
emphasis on the assays that detect lipid peroxidation, protein oxidation, and oxidative DNA base
modifications. The chapter ends with a discussion of biomarkers of oxidative stress and their value in
assessing disease pathophysiology as well as the efficacy of antioxidant intervention.
Biomarkers, DNA base modifications, DNA damage, F2-isoprostanes, Lipid peroxidation,
Methodologies, Oxidative stress, Protein oxidation, Reactive nitrogen species, Reactive oxygen species.
Virginia Tech Corporate Research Center Blacksburg, Virginia USA