Recent Patents on Chemical Engineering

Yousheng Tao  
State Key Laboratory of Structural Chemistry
Fujian Institute of Research on the Structure of Matter
Chinese Academy of Sciences
Fuzhou
China

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Engineering of Efficient Escherichia coli Excretion Systems for the Production of Heterologous Proteins for Commercial Applications

Author(s): W.K.R. Wong, Zhibiao Fu, Yule Y. Wang, K.L. Ng and Anthony K.N. Chan

Affiliation: Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

Keywords: Diabetic foot ulcers, Escherichia coli, excretion, hEGF, heterologous proteins, periplasm, secretion, SecYEG pathway, skincare applications.

Abstract:

Extracellular production of recombinant proteins using bacterial systems such as Escherichia coli has many advantages, including preferred protein properties, e.g. N-terminal authenticity, correct disulfide linkages, high product bioactivity and stability, and simplified downstream processing. This approach offers an impressive choice to the expression of naturally secretory proteins of which posttranslational modifications are non-essential for biological functions. In this communication, we review recent advances and patents obtained in excretory production of recombinant proteins in E. coli, making use of human epidermal growth factor (hEGF) as a model protein to illustrate how this approach has facilitated efficient production of hEGF. This enables affordable supply for an elaborate assessment of the biological functions of hEGF and subsequently its application to the development of a wide range of skincare and medically-oriented products for commercial applications.

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Article Details

VOLUME: 5
ISSUE: 1
Page: [45 - 55]
Pages: 11
DOI: 10.2174/2211334711205010045