Laboratory Dialysis-Past, Present and Future

Parikshit      Bansal; Dara      Ajay

Abstract

Laboratory dialysis, one of the most widely used techniques in biological research is truly a ‘gateway technology’. The analogy is to that of a ‘gate’ of a building through which everybody has to pass, even though they may wish to go to different departments. Similarly, researchers may be working in altogether different areas but all may need to use laboratory dialysis at one stage or the other during the course of their research. Biochemists may use it to purify enzymes, an immunologist may use it to purify monoclonal antibodies from culture supernatants, a chemist may use it as a step in the crystallography process or for purification of ionic liquids, a biotechnologist may use it to study the effectiveness of enzyme immobilization and a drug discovery scientist may use it for determining drug-protein interaction. The present article reviews patents in the field of laboratory dialysis from inception till date, focusing on the various developmental and innovation related milestones during evolution of the technique. It captures the full panorama of a very interesting technique which continues to be as relevant today as it was in 1866 when the term ‘dialysis’ was first coined.

Keywords: Laboratory dialysis, hemodialysis, microdialysis, macrodialysis, equilibrium dialysis, patents, dialysis apparatus, drug binding and release studies.