Persistence of Trypanosoma cruzi in Experimental Chagasic Cardiomyopathy
Triquel M. Fernanda,
Lo Presti M. Silvina,
Rivarola H. Walter.
The mechanisms by which patients infected with T. cruzi develop or not the disease are not completely understood.
We investigated the importance of parasite persistence as a factor in generating cardiomyopathy. Fifty trypomastigote forms
from the Tulahuen strain were used as inoculum for 160 Albino Swiss mice. Parasitemia, survival, histopathological and electrocardiographic
studies were done on days 90, 180, 270 and 360 post infection (pi). Parasite presence in the tissues was
determined by immunofluorescence. Maximum parasitemia was observed on day 21pi and became negative on day 42pi. By
day 90pi, 50% of the infected mice presented electrocardiographic alterations and 67% by day 360pi; the non infected group
of mice didn’t present elecrocardiographic alterations. Inflammatory infiltrates were observed throughout the infection, with a
higher percentage in those mice with electrocardiographic alterations. The presence of the parasite was observed throughout
the infection in skeletal muscle samples and on day 90pi in the myocardium. The highest mortality occurred on day 21pi, surviving
10% of the mice until the end of the experiments. These results demonstrate the presence of the parasite in the host tissues
along the infection which could produce the injuries found in the myocardium and the electrocardiographic alterations.
Keywords: Trypanosoma cruzi, chagasic cardiomyopathy, persistence
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