Evaluation of In Vivo and In Vitro Antimicrobial Activities of a Selective Serotonin Reuptake Inhibitor Sertraline Hydrochloride
Ananda Dulal Jana,
Jorn B. Christensen,
Jette Elisabeth Kristiansen.
We investigated the in vitro and in vivo antimicrobial potential of a selective serotonin reuptake inhibitor sertraline
hydrochloride, against clinical isolates of bacteria and Candida species. Minimum Inhibitory Concentration of sertraline
against 161 human isolates of 12 Gram negative and 5 Gram positive genera, Candida albicans ATCC10231 and
Candida tropicalis ATCC13803 was determined by spot inoculation, broth and agar dilution methods along with its postantibiotic
effect following a short drug exposure. The toxicity and protective efficacy was tested in vivo with a mousevirulent
strain of Salmonella typhimurium NCTC74.The MIC was 20-200μg/ml for bacteria, and 200μg/ml for Candida.
The growth inhibitory study with Bacillus subtilis UC564 and Shigella dysenteriae NCTC599/52 revealed that the drug is
bacteriostatic at its MIC and cidal at higher concentrations. Study on its post-antibiotic effect following a short drug exposure
revealed that Sertraline has a time- and dose-dependent effect. Treatment for 4h at concentrations below and equipotent
to the minimal static concentration resulted in a lag of regrowth at 8-24h for Candida isolates. The in vivo study with
Salmonella typhimurium in Swiss mice showed that the median lethal dose was 1.9 x 107 cfu and 50 LD50 was 0.95x109.
Interestingly, the drug at a non-toxic single dose provides significant protection (P<0.001) to the mice challenged with
S. typhimurium. Sertraline remarkably reduced the number of viable bacteria in organ homogenates and blood of the
treated animals and demonstrate both in vitro and in vivo antibacterial potential at lower dose than its predesignated pharmacological
Keywords: Sertraline hydrochloride, SSRI, antibacterial activity, antifungal activity, non-antibiotic
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