Abstract
The thrombin binding aptamer (TBA) is a well characterized chair-like, antiparallel quadruplex structure that binds specifically to thrombin at nanomolar concentrations and therefore it has interesting anticoagulant properties. In this article we review the research involved in the development of new TBA derivatives with improved anticoagulant properties as well as the use of the TBA as a model compound for the study of quadruplex structures. Specifically, we describe the impact of modified nucleosides and non-natural backbones in the guanine tetrads or in the loops and the introduction of pendant groups at the 3’ or 5’-ends. The modified oligonucleotides are shown to be excellent tools for the understanding of the molecular structure of the TBA and its folding properties. Finally, we review the use of the TBA-Thrombin recognition system for the development of analytical tools based on the TBA folding.
Keywords: Thrombin binding aptamer, TBA, G-quadruplex, thrombin, anticoagulant, oligonucleotide synthesis, DNA, aptamer, nanomolar, spectroscopy
Current Pharmaceutical Design
Title:Thrombin Binding Aptamer, More than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications
Volume: 18 Issue: 14
Author(s): Anna Avino, Carme Fabrega, Maria Tintore and Ramon Eritja
Affiliation:
Keywords: Thrombin binding aptamer, TBA, G-quadruplex, thrombin, anticoagulant, oligonucleotide synthesis, DNA, aptamer, nanomolar, spectroscopy
Abstract: The thrombin binding aptamer (TBA) is a well characterized chair-like, antiparallel quadruplex structure that binds specifically to thrombin at nanomolar concentrations and therefore it has interesting anticoagulant properties. In this article we review the research involved in the development of new TBA derivatives with improved anticoagulant properties as well as the use of the TBA as a model compound for the study of quadruplex structures. Specifically, we describe the impact of modified nucleosides and non-natural backbones in the guanine tetrads or in the loops and the introduction of pendant groups at the 3’ or 5’-ends. The modified oligonucleotides are shown to be excellent tools for the understanding of the molecular structure of the TBA and its folding properties. Finally, we review the use of the TBA-Thrombin recognition system for the development of analytical tools based on the TBA folding.
Export Options
About this article
Cite this article as:
Avino Anna, Fabrega Carme, Tintore Maria and Eritja Ramon, Thrombin Binding Aptamer, More than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications, Current Pharmaceutical Design 2012; 18 (14) . https://dx.doi.org/10.2174/138161212799958387
DOI https://dx.doi.org/10.2174/138161212799958387 |
Print ISSN 1381-6128 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4286 |
Call for Papers in Thematic Issues
"Tuberculosis Prevention, Diagnosis and Drug Discovery"
The Nobel Prize-winning discoveries of Mycobacterium tuberculosis and streptomycin have enabled an appropriate diagnosis and an effective treatment of tuberculosis (TB). Since then, many newer diagnosis methods and drugs have been saving millions of lives. Despite advances in the past, TB is still a leading cause of infectious disease mortality ...read more
Current Pharmaceutical challenges in the treatment and diagnosis of neurological dysfunctions
Neurological dysfunctions (MND, ALS, MS, PD, AD, HD, ALS, Autism, OCD etc..) present significant challenges in both diagnosis and treatment, often necessitating innovative approaches and therapeutic interventions. This thematic issue aims to explore the current pharmaceutical landscape surrounding neurological disorders, shedding light on the challenges faced by researchers, clinicians, and ...read more
Emerging and re-emerging diseases
Faced with a possible endemic situation of COVID-19, the world has experienced two important phenomena, the emergence of new infectious diseases and/or the resurgence of previously eradicated infectious diseases. Furthermore, the geographic distribution of such diseases has also undergone changes. This context, in turn, may have a strong relationship with ...read more
Melanoma and Non-Melanoma Skin Cancer Treatment: Standard of Care and Recent Advances
In this thematic issue, we aim to provide a standard of care of the diagnosis and treatment of melanoma and non-melanoma skin cancer. The editor will invite authors from different countries who will write review articles of melanoma and non-melanoma skin cancers. The Diagnosis, Staging, Surgical Treatment, Non-Surgical Treatment all ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Specificities of the Diabetic Population in French Guiana: The Health Barometer Survey
Current Diabetes Reviews Quantization of Angiogenesis and Image Analysis
Current Angiogenesis (Discontinued) Synthesis and Evaluation of Heterocyclic Scaffold-Based Styrene Conjugates as New Antibacterial Agents
Anti-Infective Agents Review of the Third Domain Receptor Binding Fragment of Alphafetoprotein (AFP): Plausible Binding of AFP to Lysophospholipid Receptor Targets
Current Drug Targets Copper Status Abnormalities and How to Measure Them in Neurodegenerative Disorders
Recent Patents on CNS Drug Discovery (Discontinued) Exhaled Breath Biomonitoring Using Laser Spectroscopy
Current Analytical Chemistry Hepatoprotective Effects of Algerian Crataegus oxyacantha Leaves
Recent Patents on Food, Nutrition & Agriculture Endogenous Glutathione Adducts
Current Drug Metabolism Cheminformatics Based Machine Learning Approaches for Assessing Glycolytic Pathway Antagonists of Mycobacterium tuberculosis
Combinatorial Chemistry & High Throughput Screening Advancing Metabolomics Research and Biomarker Application with Nanotechnology
Current Metabolomics β-Aminocarbonyl Compounds: Chemistry and Biological Activities
Mini-Reviews in Organic Chemistry Quantifying Reinforcement Value and Demand for Psychoactive Substances in Humans
Current Drug Abuse Reviews Potential Advantages of Using Synchrotron X-ray Based Techniques in Pediatric Research
Current Medicinal Chemistry Lectin Microarrays: A Powerful Tool for Glycan-Based Biomarker Discovery
Combinatorial Chemistry & High Throughput Screening Novel 1,3,4-Thiadiazole Linked Amide Derivatives of Pteridone: Synthesis and Study of Anticancer Activities
Letters in Organic Chemistry 2D QSAR Studies on a Series of Quinazoline Derivatives as Tyrosine Kinase (EGFR) Inhibitor: An Approach to Design Anticancer Agents
Letters in Drug Design & Discovery Synthesis and Characterization of Some New Thiazole based Thiazolidinone Derivatives as Potent Antimicrobial and Antimycobacterial Agents
Anti-Infective Agents Application of Microfluidics to the Ultra-Rapid Preparation of Fluorine-18 Labelled Compounds
Current Radiopharmaceuticals Using Measured pKa, LogP and Solubility to Investigate Supersaturation and Predict BCS Class
Current Drug Metabolism 31P NMR Spectroscopy of Phospholipids: From Micelles to Membranes
Current Analytical Chemistry