Topical Gene Delivery in Mice Using Gemini Surfactant - Lipid Nanoparticles With and Without Tape Electrode Electroporation

Author(s): I. Badea, N. Shaterian, M. Foldvari.

Journal Name: Drug Delivery Letters

Volume 1 , Issue 1 , 2011

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Abstract:

Gemini surfactant-lipid cationic nanoparticles (NP) and electroporation (EP) with a novel tape-electrode were evaluated in mice as topical delivery methods for plasmid DNA encoding luciferase reporter gene or interferon gamma (IFNg). NP treatment of the skin without EP (dose: 25 microgram plasmid DNA/50 microliter formulation) enhanced skin expression of IFNg, three-fold compared to buffer control or topical plasmid solution, whereas application of the noninvasive tape-probe to deliver the electroporative effect after topical NP treatment decreased gene expression compared with topical NP treatment alone. Gemini NPs are a potential topical gene delivery system, however subsequent use of EP showed an inhibitory effect.

Keywords: Electroporation, gene therapy, nanoparticles, non-viral, plasmid, skin, topical, Gemini surfactant-lipid cationic nanoparticles, NP treatment

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Article Details

VOLUME: 1
ISSUE: 1
Year: 2011
Page: [62 - 66]
Pages: 5
DOI: 10.2174/2210304x11101010062

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