Group A Streptococcal Vaccine Candidates based on the Conserved Conformational Epitope from M Protein
Mariusz Skwarczynski, Abdullah A. H. Ahmad Fuaad, Lina Rustanti, Zyta M. Ziora, Mohammed Aqil, Michael R Batzloff, Michael F. Good and Istvan Toth
Affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland, Australia.
Copper catalysed azide alkyne Huisgen cycloaddition (click) reaction assisted conjugation of the multiple copy of conserve epitope (J14) derived from Group A Streptococcal M-protein to LCP core induced desired α-helical conformation of the epitope. The constructs were able to self assemble into large nanoparticles under aqueous conditions. Immunological assessment of these particles demonstrated their capacity to elicit high-titer systemic IgG antibodies against the epitope without help of adjuvant. The LCP systems incorporating the epitope via its N- or C-termini did not elicit significantly different immune responses.
Keywords: Epitope orientation, group A streptococcus, infectious disease, lipid core peptide, nanoparticles, self-adjuvanting, lipopeptide vaccine, N-TERMINUS J14 AZIDE, HUISGEN CYCLOADDITION, LCP CORE
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