Group A Streptococcal Vaccine Candidates based on the Conserved Conformational Epitope from M Protein
Abdullah A. H. Ahmad Fuaad,
Zyta M. Ziora,
Michael R Batzloff,
Michael F. Good,
Copper catalysed azide alkyne Huisgen cycloaddition (click) reaction assisted conjugation of the multiple copy of conserve epitope (J14) derived from Group A Streptococcal M-protein to LCP core induced desired α-helical conformation of the epitope. The constructs were able to self assemble into large nanoparticles under aqueous conditions. Immunological assessment of these particles demonstrated their capacity to elicit high-titer systemic IgG antibodies against the epitope without help of adjuvant. The LCP systems incorporating the epitope via its N- or C-termini did not elicit significantly different immune responses.
Keywords: Epitope orientation, group A streptococcus, infectious disease, lipid core peptide, nanoparticles, self-adjuvanting, lipopeptide vaccine, N-TERMINUS J14 AZIDE, HUISGEN CYCLOADDITION, LCP CORE
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