Abstract
To study the relationship between aging and the vulnerability of substantia nigra pars compacta (SNc) tyrosine hydroxylase immunoreactive positive (TH+) dopaminergic (DA) neurons. We determined the number of TH+ DA neurons in aged rats (24 mon) compared to adult rats (5 mon) using immunohistochemistry and cell counting. Furthermore, the expression of TH mRNA and protein levels in SN was studied by semi-quantitative RT-PCR and Western blotting. A 13.6% loss of neurons was detected in rostral segment of SNc, and the expression of TH mRNA levels was also reduced (P < 0.05), however, no difference was detected in TH protein levels (P > 0.05). These data suggest that expression of TH protein may increase in the existing SNc DA neurons, which may compensate for the partial loss of TH+ DA neurons.
Keywords: Aging, tyrosine hydroxylase, substantia nigra pars compacta, Parkinson's disease, dopaminergic (DA) neurons, immunohistochemistry, Hydroxylase, dopamine, alpha-synuclein, monoamine transporter, nigrostriatal system, alkaline phosphatase (AP)-conjugated, horseradish peroxidase (HRP)-conjugated, stereomicroscope, nitrocellulose membrane, serum albumin, IgG, Elite avidin-biotin complex, 3'-diaminobenzidine (DAB), Stereological Analysis, Statistical Analysis, anti-TH antibody and cytometry, Immunoblotting, vascular endothelial growth factor (VEGF), mRNA, synapotosomes, calbindin
Current Aging Science
Title: Influence of Aging on the Dopaminergic Neurons in the Substantia Nigra Pars Compacta of Rats
Volume: 4 Issue: 1
Author(s): Jin Gao, Hui Miao, Cheng-hua Xiao, Yu Sun, Xia Du, Hong-hua Yuan, Hong-li Yu and Dian-shuai Gao
Affiliation:
Keywords: Aging, tyrosine hydroxylase, substantia nigra pars compacta, Parkinson's disease, dopaminergic (DA) neurons, immunohistochemistry, Hydroxylase, dopamine, alpha-synuclein, monoamine transporter, nigrostriatal system, alkaline phosphatase (AP)-conjugated, horseradish peroxidase (HRP)-conjugated, stereomicroscope, nitrocellulose membrane, serum albumin, IgG, Elite avidin-biotin complex, 3'-diaminobenzidine (DAB), Stereological Analysis, Statistical Analysis, anti-TH antibody and cytometry, Immunoblotting, vascular endothelial growth factor (VEGF), mRNA, synapotosomes, calbindin
Abstract: To study the relationship between aging and the vulnerability of substantia nigra pars compacta (SNc) tyrosine hydroxylase immunoreactive positive (TH+) dopaminergic (DA) neurons. We determined the number of TH+ DA neurons in aged rats (24 mon) compared to adult rats (5 mon) using immunohistochemistry and cell counting. Furthermore, the expression of TH mRNA and protein levels in SN was studied by semi-quantitative RT-PCR and Western blotting. A 13.6% loss of neurons was detected in rostral segment of SNc, and the expression of TH mRNA levels was also reduced (P < 0.05), however, no difference was detected in TH protein levels (P > 0.05). These data suggest that expression of TH protein may increase in the existing SNc DA neurons, which may compensate for the partial loss of TH+ DA neurons.
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Cite this article as:
Gao Jin, Miao Hui, Xiao Cheng-hua, Sun Yu, Du Xia, Yuan Hong-hua, Yu Hong-li and Gao Dian-shuai, Influence of Aging on the Dopaminergic Neurons in the Substantia Nigra Pars Compacta of Rats, Current Aging Science 2011; 4 (1) . https://dx.doi.org/10.2174/1874609811104010019
DOI https://dx.doi.org/10.2174/1874609811104010019 |
Print ISSN 1874-6098 |
Publisher Name Bentham Science Publisher |
Online ISSN 1874-6128 |
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