Application of Outer Membrane Vesicle of Neisseria meningitidis Serogroup B as a New Adjuvant to Induce Strongly Th1-Oriented Responses Against HIV-1

Author(s): Mohammad Reza Aghasadeghi, Ali Sharifat Salmani, Seyed Mehdi Sadat, Foozieh Javadi, Arash Memarnejadian, Rouhoullah Vahabpour, Rezvan Zabihollahi, Arfa Moshiri, Seyed Davar Siadat.

Journal Name: Current HIV Research

Volume 9 , Issue 8 , 2011

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Abstract:

Despite the worldwide efforts made in the field of HIV vaccine development, an efficient AIDS vaccine strategy is still vague. Virus-like particles (VLPs) are one of the introduced aspects for HIV vaccine development since the non-replicative nature of HIV VLPs, resulting from the lack of viral genomic RNA, makes them suitable for broad applications. We have previously designed and introduced non-infectious VLPs (mzNL4-3) by introduction of a deletion mutation in the reverse transcriptase and integrase coding regions of HV-1. There are evidences suggesting that an effective cellular immune response against HIV-1 is able to control and suppress viremia during primary and chronic HIV infections. In the present study we have evaluated the potency of mzNL4-3 VLPs mixed with Neisseria meningitidis serogroup B outer-membrane vesicle (OMV), which is among the microbial components with proved adjuvant properties, to induce humoral and cellular responses against HIV-1. Analysis of anti-HIV-1 responses elicited in immunized BALB/c mice following different immunization regimens indicated OMV+VLP as an immunopotent combination which significantly induced anti-HIV-1 IgG with IgG2a dominancy. Results of cytokine and ELISpot assays also showed the capability of VLP+OMV immunogen for effective induction of IFN-gamma; and IL4 secreting cells and further suggested the promotion of Th1-oriented response that was evidenced with the increased IFN-γ/IL4 secretion ratio. According to our study, HIV-1 VLPs combined with N. meningitidis B OMVs seem to be a promising approach in vaccine development against HIV-1.

Keywords: HIV-1, VLP, Neisseria meningitidis, OMV, Cell mediated immunity, Th 1, antigens, HBsAg, Virus Like Particles, Cytokine

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Article Details

VOLUME: 9
ISSUE: 8
Year: 2011
Page: [630 - 635]
Pages: 6
DOI: 10.2174/157016211798998772
Price: $65

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