Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

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A Luminescence Study of the Interaction of Sulfobutylether-β-Cyclodextrin with Rutin

Author(s): Min Wu, Zhenghua Song, Jingjing Zhang.

Abstract:

The luminol/sulfobutylether-β-cyclodextrin (SBE7M-β-CD) chemiluminescence (CL) system and the interaction of SBE7M-β-CD/rutin were first described by flow injection (FI) CL method. It was found that SBE7M-β-CD with luminol could form 1:1 complex online, which could accelerate the electrons transferring rate of excited 3-aminophthalate, giving the enhanced CL intensity of luminol. The enhancement of CL intensity was proportional to the concentrations of SBE7M- β-CD with a linear range from 25 to 1750 μmol L-1. It was also found that rutin could inhibit the CL intensity from luminol/SBE7M-β-CD system, and the decrement of CL intensity was logarithm over the concentrations of rutin ranging from 0.1 to 100.0 nmol L-1, giving the regression equation ΔI = 32.90lgCrutin + 16.26 (R2 = 0.9952) with a detection limit of 0.03 nmol L-1 (3σ). According to the proposed CL model, the binding constant (KCD-R) and the stoichiometric ratio of SBE7M-β-CD/rutin complex were obtained as 1.6 × 106 L2 mol-2 and 2:1. The possible mechanism of luminol/SBE7M-β- CD/rutin interaction was also discussed. The method was successfully applied to monitor rutin in human urine samples after ingesting SBE7M-β-CD/rutin complex, with a total excretion of 68.8% within 8.0 h.

Keywords: Chemiluminescence, flow injection, luminal, rutin, pharmacokinetics, sulfobutylether-β-cyclodextrin, Cyclodextrins, oligosaccharides, bioavailability, photochemical reaction

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Article Details

VOLUME: 5
ISSUE: 4
Year: 2011
Page: [259 - 266]
Pages: 8
DOI: 10.2174/187231211798472520
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