Current Proteomics

Bernd Rehm  
Institute of Molecular BioSciences Massey University
Private Bag 11222
Palmerston North
New Zealand

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Monosaccharide Compositional Analysis of Glycoproteins Using Liquid Chromatography-Mass Spectrometry

Author(s): Loubna A. Hammad, Yehia Mechref.

Abstract:

Effective and accurate analysis of free monosaccharides and monosaccharides bound to glycoconjugates, including glycoproteins and glycolipids have been achieved through the development of different analytical methods. Although all of these analytical methods appears to be applicable to the quantification of monosaccharides, quantification through coupling of liquid chromatography to mass spectrometry (LC-MS) is currently considered to be one the most effective analytical methods. This review focuses on the development of LC-MS methods for the analysis of monosaccharides, and evaluates the different analytical approaches including various ionization and detection techniques that have been used in order to analyze and quantify monosaccharides using mass spectrometry.

Keywords: monosaccharides, Glycans, High-performance liquid chromatography, LC-MS, glycoconjugates, glycoproteins, mass spectrometry, liquid chromatography, glycosylation, mammalian diseases

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Article Details

VOLUME: 8
ISSUE: 4
Year: 2011
Page: [257 - 268]
Pages: 12
DOI: 10.2174/157016411798220835
Price: $58