Current Proteomics

Bernd Rehm  
Institute of Molecular BioSciences Massey University
Private Bag 11222
Palmerston North
New Zealand

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Abundance- and Activity-Based Proteomics in Platelet Biology

Author(s): Stephen P. Holly, Xian Chen, Leslie V. Parise.

Abstract:

Human platelets are thought to express approximately 2000-3000 proteins, but post-translational modifications, alternatively spliced variants and a rich diversity of vertebrate domain architectures likely make this a conservative estimate. Even though rapidly advancing proteomic techniques have catalyzed the identification of roughly one third of the platelet proteome, a combination of abundance-based and activity-based proteomics is needed for elucidation of platelet functional characteristics including the definition of a “core proteome” and recognition of diverse enzyme activity profiles associated with various physiological states. In this review, we describe the latest mass spectrometry-based techniques capable of providing some of these physiological details required for more comprehensive evaluation of the human platelet repertoire.

Keywords: ABPP, biomarker, cancer, library, mass spectrometry, platelet, proteome, rat, hemostasis, interaction, interactome, protein, thrombosis, megakaryocyte, low-abundant proteins, cytoskeleton, vasodilator-stimulated, phosphorylations, kinase substrates, Glanzmann thrombasthenia

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Article Details

VOLUME: 8
ISSUE: 3
Year: 2011
Page: [216 - 228]
Pages: 13
DOI: 10.2174/157016411797247512
Price: $58