Drug Metabolism Letters

Zhiyang Zhao
Cambridge, MA


Tracing Fungi Secondary Metabolites in Brazil Nuts Using LC-MS/MS

Author(s): Otniel Freitas-Silva, Maria de Lourdes Mendes de Souza, Armando Venancio.


This screening aimed to evaluate quantitatively the occurrence of fungal metabolites in Brazil nuts. Nuts were collected from Agroforest production areas in Amazon basin region. A total of 235 mycotoxins (including the most prominent ones) was screened by a multi-mycotoxin method based on HPLC-MS/MS. The recovery of metabolites by the method was between 56 and 136%. Fifteen mycotoxins were detected and quantified, in at least one sample; namely, aflatoxins (AFB1, AFB2, AFG1, and AFM1), sterigmatocystin, methyl-sterigmatocystin, kojic acid, citrinin, cyclosporin A, cyclosporin C, cyclosporin D, cyclosporin H, rugulosin, alternariol-methylether and emodin. This is the first study dealing with the detection of the latter nine metabolites in Brazil nuts. Alternariol-methylether (from 0.75 to 3.2 μg/kg) was the only metabolite detected in all analyzed samples.

Keywords: aflatoxins, alternariol-methylether, Aspergillus spp., Bertholethia excelsa, fungal metabolites, kojic acid, Multimycotoxin, Brazil nut, Lecythidaceae, acetonitrile

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Article Details

Year: 2011
Page: [150 - 155]
Pages: 6
DOI: 10.2174/187231211796905053
Price: $58