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Current Drug Discovery Technologies
ISSN (Print): 1570-1638
ISSN (Online): 1875-6220
VOLUME: 8
ISSUE: 2
DOI: 10.2174/157016311795563893      Price:  $58









The Activity of Matrix Metalloproteinase-9 is Part of the Mechanism of Cell-to-Cell HIV-1 Endocytosis in Dendritic Cells

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Author(s): Eliana Ruggiero, Elena Toschi and Maurizio Federico
Pages 112-118 (7)
Abstract:
Human immunodeficiency virus (HIV) starts to replicate upon virus-cell fusion mediated by the CD4-Envcoreceptor interaction. HIV enters target cells also through endocytosis, a mechanism which rarely leads to HIV replication. Both modes of entry are greatly improved by cell-cell contact. We recently reported that the contact of human primary dendritic cells with HIV-1 infected cells leads to high levels of virus endocytosis and HIV-1 antigen presentation activity in dendritic cells. Here, we provide evidence that the activity of matrix metalloproteinase (MMP)-9 is involved in the mechanism of cell-to-cell HIV-1 endocytosis in DCs. Accordingly, the specific inhibition of MMP-9 led to reduced extents of HIV-1 antigen presentation activity. The identification of cell molecules involved in the cell-to-cell HIV-1 endocytosis would be of significance for better understanding the mechanisms underlying the induction of the anti-HIV adaptive immune response.
Keywords:
Cell-to-cell HIV-1 endocytosis, dendritic cells, GM6001, matrix metalloproteinase-9, siRNAs, antigen presentation activity
Affiliation:
National AIDS Center, Istituto Superiore di Sanita, Viale Regina Elena, 299, 00161 Rome, Italy.