Current Proteomics

Bernd Rehm  
Institute of Molecular BioSciences Massey University
Private Bag 11222
Palmerston North
New Zealand

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Novel Sample Preparation for Mass Spectral Analysis of Complex Biological Samples

Author(s): Eric A. Porsch, Cecelia A. Shertz, Michael D. Boyle.

Abstract:

The ability to combine a selective capture strategy with on chip MALDI-TOF analysis allows for rapid, sensitive analysis of a variety of different analytes. In this overview a series of applications of capture enhanced laser desorption ionization time of flight (CELDI-TOF) mass spectrometry are described. The key feature of the assay is an off-chip capture step that utilizes high affinity bacterial binding proteins to capture a selected ligand. This allows large volumes of sample to be used and provides for a concentration step prior to transfer to a gold chip for traditional mass spectral analysis. The approach can also be adapted to utilize specific antibody as the basis of the capture step. The direct and indirect CELDI-TOF assays are rapid, reproducible and can be a valuable proteomic tool for analysis of low abundance molecules present in complex mixtures like blood plasma.

Keywords: CELDI-TOF capture-enhanced laser desorption ionization time of flight mass spectroscopy, IdeS, Immunoglobulin G: albumin, cysteine proteases

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Article Details

VOLUME: 7
ISSUE: 2
Year: 2010
Page: [90 - 101]
Pages: 12
DOI: 10.2174/157016410791330589
Price: $58