Abstract
Proper cellular functioning is dependent on the timely association of thousands of proteins in discrete complexes. A major challenge in the post genomic era is the elucidation of these diverse protein complexes and networks that govern mammalian cellular function and cell fate. Identification of the individual components of these arrangements is vital to the understanding of their function in biological processes. Currently, a widely used technique for the identification of protein complexes is a combinational approach of Tandem Affinity Purification coupled with Mass Spectrometry (TAP-MS). This technique was originally developed for the study of the yeast proteome but has since been adapted for studies of the mammalian system. This review discusses the applications of TAP-MS to the mammalian proteome. Our focus includes the technical improvements made in each step of the TAP-MS pipeline including vector design, delivery of the TAP construct into cells, expression of bait proteins, purification strategies and finally the identification of the protein complexes by mass spectrometry.
Keywords: Tandem affinity purification, mass spectrometry, protein complexes, protein purification
Current Proteomics
Title: Advances in the Study of Mammalian Proteome by Tandem Affinity Purification – Mass Spectrometry
Volume: 7 Issue: 1
Author(s): Vahab D. Soleimani and Michael A. Rudnicki
Affiliation:
Keywords: Tandem affinity purification, mass spectrometry, protein complexes, protein purification
Abstract: Proper cellular functioning is dependent on the timely association of thousands of proteins in discrete complexes. A major challenge in the post genomic era is the elucidation of these diverse protein complexes and networks that govern mammalian cellular function and cell fate. Identification of the individual components of these arrangements is vital to the understanding of their function in biological processes. Currently, a widely used technique for the identification of protein complexes is a combinational approach of Tandem Affinity Purification coupled with Mass Spectrometry (TAP-MS). This technique was originally developed for the study of the yeast proteome but has since been adapted for studies of the mammalian system. This review discusses the applications of TAP-MS to the mammalian proteome. Our focus includes the technical improvements made in each step of the TAP-MS pipeline including vector design, delivery of the TAP construct into cells, expression of bait proteins, purification strategies and finally the identification of the protein complexes by mass spectrometry.
Export Options
About this article
Cite this article as:
Soleimani D. Vahab and Rudnicki A. Michael, Advances in the Study of Mammalian Proteome by Tandem Affinity Purification – Mass Spectrometry, Current Proteomics 2010; 7 (1) . https://dx.doi.org/10.2174/157016410790979626
DOI https://dx.doi.org/10.2174/157016410790979626 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
Call for Papers in Thematic Issues
Mass spectrometry data acquisition and analysis for proteomics
The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
Peptides: State-of-Art and Commercialisation Hurdles
The Editors of the Current Proteomics (CP) journal are highly privileged to welcome scientists to submit their scientific research and review articles to be considered for publication in the upcoming thematic issue. The topics should cover various aspects of peptides in regard to their synthetic methodologies, formulation approaches, pharmacological challenges, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Advances of Inorganic Materials in the Detection and Therapeutic Uses against Coronaviruses
Current Medicinal Chemistry Anatomical Approach to Clinical Problems of Popliteal Fossa
Current Rheumatology Reviews Disease Progression in Children with Vertically-Acquired HIV Infection in Sub-Saharan Africa: Reviewing the Need for HIV Treatment
Current HIV Research Editorial [Hot Topic:New Research Tolls for Antique Pediatric Tumors:The Dilemma of Transposing Drug-Resistance (Guest Editor: E.T. Valera)]
Current Pharmaceutical Biotechnology Can Targeting the Incretin Pathway Dampen RAGE-Mediated Events in Diabetic Nephropathy?
Current Drug Targets Advancement Towards Tin-based Anticancer Chemotherapeutics: Structural Modification and Computer Modeling Approach to Drug-Enzyme Interactions
Current Topics in Medicinal Chemistry The Structure and Functions of P-Glycoprotein
Current Medicinal Chemistry In Vitro and In Vivo Approaches for Screening the Potential of Anticancer Agents: A Review
Current Drug Discovery Technologies Diaryl Urea: A Privileged Structure in Anticancer Agents
Current Medicinal Chemistry Potential Therapeutic Benefits of Maintaining Mitochondrial Health in Peripheral Neuropathies
Current Neuropharmacology Carcinogenesis and Diabetic Wound Healing: Evidences of Parallelism
Current Diabetes Reviews Bioactive Compounds Containing Benzoxadiazole, Benzothiadiazole, Benzotriazole
Current Bioactive Compounds Chronic Myelogenous Leukemia
Current Cancer Therapy Reviews Is Resistance Futile?
Current Drug Targets - Infectious Disorders Insulin-Like Growth Factor 2 - The Oncogene and its Accomplices
Current Pharmaceutical Design Drug-Related Cardiotoxicity for the Treatment of Haematological Malignancies in Elderly
Current Pharmaceutical Design Emulsomes Meet S-layer Proteins: An Emerging Targeted Drug Delivery System
Current Pharmaceutical Biotechnology Transition Metal-Based Prodrugs for Anticancer Drug Delivery
Current Medicinal Chemistry “Bedside-to-Bench” Behavioral Outcomes in Animal Models of Pain: Beyond the Evaluation of Reflexes
Current Neuropharmacology Novel Patents Targeting Interleukin-17A; Implications in Cancer and Inflammation
Recent Patents on Anti-Cancer Drug Discovery