Recent Patents on Biotechnology

Rongling Wu
Departments of Public Health Sciences and Statistics
Pennsylvania State College of Medicine
Hershey, PA


Secretory Production of Recombinant Proteins in Escherichia coli

Author(s): Sung Ho Yoon, Seong Keun Kim and Jihyun F. Kim

Affiliation: KRIBB, 111 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea.


Extracellular production of heterologous proteins using the Escherichia coli cell factory offers several advantages over intracellular production and mammalian culture. Properly folded proteins can be rapidly accumulated in the culture media, and downstream processes for isolation and purification can be much simplified. Efforts to enhance the secretory production of target proteins can be largely classified as selection and modification of the signal peptide, coexpression of proteins to assist translocation and folding, improvement of periplasmic release, and protection of target proteins from degradation and contamination. Here, we review recent patents on the secretory production of recombinant proteins in E. coli.

Keywords: Secretion, excretion, periplasm, extracellular production, signal peptide, recombinant protein, Escherichia coli

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Article Details

Page: [23 - 29]
Pages: 7
DOI: 10.2174/187220810790069550